Abundance of questing ticks and molecular evidence for pathogens in ticks in three parks of Emilia-Romagna region of Northern Italy

Sara Aureli, Roberta Galuppi, Fabio Ostanello, Janet E Foley, Cristina Bonoli, Daniel Rejmanek, Giorgia Rocchi, Elisa Orlandi, Maria Paola Tampieri

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Introduction and objective. Infectious and parasitic diseases transmitted by ticks, such as Lyme diseases, granulocytic anaplasmosis and piroplasmosis, have been frequently reported in Europe, with increasing attention to them as an emerging zoonotic problem. The presented study was performed to assess the distribution and the density of questing ticks in three regional parks of Emilia-Romagna region of Northern Italy, and to seek molecular evidence of potential human pathogens in tick populations. Materials and Methods. In the period April-October 2010, 8,139 questing ticks were collected: 6,734 larvae, 1,344 nymphs and only a few adults – 28 females and 33 males. The abundance of Ixodes ricinus questing ticks was compared among different sampling sites and related to microclimate parameters. 1,544 out of 8,139 ticks were examined for the presence of pathogens: PCR was used to detect piroplasms DNA and Real time Taqman PCR for Anaplasma phagocytophilum and Borrelia burgdorferi s.l. Results. The predominant species was I. ricinus (overall abundance 1,075.9/100 m<sup>2</sup>); more rarely, Dermacentor marginatus (n = 37 – 0.45%), Scaphixodes frontalis (n = 13 – 0.16%), Hyalomma spp. (n = 6 – 0.07%) and Ixodes acuminatus (n = 3 – 0.04%) were also found. 28 out of 324 (8.6%) samples of ticks were PCR-positive for piroplasm DNA. 11 amplicons of 18S rRNA gene were identical to each other and had 100% identity with Babesia EU1 (Babesia venatorum) using BLAST analysis. Real time Taqman PCR gave positive results for A. phagocytophilum in 23 out of 292 samples (7.9%), and for B. burgdorferi s.l. in 78 out of 292 samples (26.7%). I. ricinus was the only species found positive for pathogens by molecular analysis; 16 tick samples were co-infected with at least 2 pathogens. Discussion. The peak of nymph presence was in May, and the higher prevalence of pathogens occurred in April-June, most often in nymphs; therefore, spring season could represent the higher risk period for the transmission of pathogens. These data could provide guidelines for the preventions of tick-trasmitted diseases in this region.

Original languageEnglish (US)
Pages (from-to)459-466
Number of pages8
JournalAnnals of Agricultural and Environmental Medicine
Volume22
Issue number3
DOIs
StatePublished - Sep 16 2015

Fingerprint

tick
Ticks
Italy
ticks
pathogen
pathogens
Ixodes
Nymph
Ixodes ricinus
Anaplasma phagocytophilum
nymphs
Piroplasmida
Babesia
Borrelia burgdorferi
Real-Time Polymerase Chain Reaction
quantitative polymerase chain reaction
sampling
Dermacentor marginatus
Anaplasmosis
Lyme disease

Keywords

  • Anaplasma phagocytophilum
  • Babesia EU1
  • Borrelia burgdorferi s.l
  • Emilia Romagna region (Italy)
  • Ixodes ricinus
  • Questing ticks

ASJC Scopus subject areas

  • Ecology, Evolution, Behavior and Systematics
  • Waste Management and Disposal
  • Public Health, Environmental and Occupational Health

Cite this

Abundance of questing ticks and molecular evidence for pathogens in ticks in three parks of Emilia-Romagna region of Northern Italy. / Aureli, Sara; Galuppi, Roberta; Ostanello, Fabio; Foley, Janet E; Bonoli, Cristina; Rejmanek, Daniel; Rocchi, Giorgia; Orlandi, Elisa; Tampieri, Maria Paola.

In: Annals of Agricultural and Environmental Medicine, Vol. 22, No. 3, 16.09.2015, p. 459-466.

Research output: Contribution to journalArticle

Aureli, Sara ; Galuppi, Roberta ; Ostanello, Fabio ; Foley, Janet E ; Bonoli, Cristina ; Rejmanek, Daniel ; Rocchi, Giorgia ; Orlandi, Elisa ; Tampieri, Maria Paola. / Abundance of questing ticks and molecular evidence for pathogens in ticks in three parks of Emilia-Romagna region of Northern Italy. In: Annals of Agricultural and Environmental Medicine. 2015 ; Vol. 22, No. 3. pp. 459-466.
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abstract = "Introduction and objective. Infectious and parasitic diseases transmitted by ticks, such as Lyme diseases, granulocytic anaplasmosis and piroplasmosis, have been frequently reported in Europe, with increasing attention to them as an emerging zoonotic problem. The presented study was performed to assess the distribution and the density of questing ticks in three regional parks of Emilia-Romagna region of Northern Italy, and to seek molecular evidence of potential human pathogens in tick populations. Materials and Methods. In the period April-October 2010, 8,139 questing ticks were collected: 6,734 larvae, 1,344 nymphs and only a few adults – 28 females and 33 males. The abundance of Ixodes ricinus questing ticks was compared among different sampling sites and related to microclimate parameters. 1,544 out of 8,139 ticks were examined for the presence of pathogens: PCR was used to detect piroplasms DNA and Real time Taqman PCR for Anaplasma phagocytophilum and Borrelia burgdorferi s.l. Results. The predominant species was I. ricinus (overall abundance 1,075.9/100 m2); more rarely, Dermacentor marginatus (n = 37 – 0.45{\%}), Scaphixodes frontalis (n = 13 – 0.16{\%}), Hyalomma spp. (n = 6 – 0.07{\%}) and Ixodes acuminatus (n = 3 – 0.04{\%}) were also found. 28 out of 324 (8.6{\%}) samples of ticks were PCR-positive for piroplasm DNA. 11 amplicons of 18S rRNA gene were identical to each other and had 100{\%} identity with Babesia EU1 (Babesia venatorum) using BLAST analysis. Real time Taqman PCR gave positive results for A. phagocytophilum in 23 out of 292 samples (7.9{\%}), and for B. burgdorferi s.l. in 78 out of 292 samples (26.7{\%}). I. ricinus was the only species found positive for pathogens by molecular analysis; 16 tick samples were co-infected with at least 2 pathogens. Discussion. The peak of nymph presence was in May, and the higher prevalence of pathogens occurred in April-June, most often in nymphs; therefore, spring season could represent the higher risk period for the transmission of pathogens. These data could provide guidelines for the preventions of tick-trasmitted diseases in this region.",
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AU - Aureli, Sara

AU - Galuppi, Roberta

AU - Ostanello, Fabio

AU - Foley, Janet E

AU - Bonoli, Cristina

AU - Rejmanek, Daniel

AU - Rocchi, Giorgia

AU - Orlandi, Elisa

AU - Tampieri, Maria Paola

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N2 - Introduction and objective. Infectious and parasitic diseases transmitted by ticks, such as Lyme diseases, granulocytic anaplasmosis and piroplasmosis, have been frequently reported in Europe, with increasing attention to them as an emerging zoonotic problem. The presented study was performed to assess the distribution and the density of questing ticks in three regional parks of Emilia-Romagna region of Northern Italy, and to seek molecular evidence of potential human pathogens in tick populations. Materials and Methods. In the period April-October 2010, 8,139 questing ticks were collected: 6,734 larvae, 1,344 nymphs and only a few adults – 28 females and 33 males. The abundance of Ixodes ricinus questing ticks was compared among different sampling sites and related to microclimate parameters. 1,544 out of 8,139 ticks were examined for the presence of pathogens: PCR was used to detect piroplasms DNA and Real time Taqman PCR for Anaplasma phagocytophilum and Borrelia burgdorferi s.l. Results. The predominant species was I. ricinus (overall abundance 1,075.9/100 m2); more rarely, Dermacentor marginatus (n = 37 – 0.45%), Scaphixodes frontalis (n = 13 – 0.16%), Hyalomma spp. (n = 6 – 0.07%) and Ixodes acuminatus (n = 3 – 0.04%) were also found. 28 out of 324 (8.6%) samples of ticks were PCR-positive for piroplasm DNA. 11 amplicons of 18S rRNA gene were identical to each other and had 100% identity with Babesia EU1 (Babesia venatorum) using BLAST analysis. Real time Taqman PCR gave positive results for A. phagocytophilum in 23 out of 292 samples (7.9%), and for B. burgdorferi s.l. in 78 out of 292 samples (26.7%). I. ricinus was the only species found positive for pathogens by molecular analysis; 16 tick samples were co-infected with at least 2 pathogens. Discussion. The peak of nymph presence was in May, and the higher prevalence of pathogens occurred in April-June, most often in nymphs; therefore, spring season could represent the higher risk period for the transmission of pathogens. These data could provide guidelines for the preventions of tick-trasmitted diseases in this region.

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