Abundance and tissue distribution of selenocysteine-containing proteins in the rat

Wayne C. Hawkes, Eric C. Wilhelmsen, Al L. Tappel

Research output: Contribution to journalArticle

81 Citations (Scopus)

Abstract

The form and distribution of selenium (Se) in proteins from selected tissues of the rat were studied by measuring 75Se radioactivity in animals provided for 5 months with [75Se]selenite as the main dietary source of Se. Equilibration of the animals to a constant specific activity of 75Se allowed the measurement of 75Se to be used as a specific elemental assay for Se. Skeletal muscle, liver and blood accounted for 73% of the whole-body Se and 95% of the total Se-dependent glutathione peroxidase activity. Over 80% of the whole-body Se was in protein in the form of the selenoamino acid, selenocysteine. All other forms of Se that were measured accounted for less than 3% of the whole-body Se. The Se in protein was distributed in seven subunit sizes and nine chromatographic forms. The Se in glutathione peroxidase accounted for one-third of the whole-body Se. These results show that the main use of dietary Se, as selenite, in rats is for the synthesis of selenocysteine-containing proteins. Furthermore, the presence of two-thirds of the whole-body Se in nonglutathione peroxidase, selenocysteine-containing proteins suggests that there may be other important mammalian selenoenzymes besides glutathione peroxidase.

Original languageEnglish (US)
Pages (from-to)77-92
Number of pages16
JournalJournal of Inorganic Biochemistry
Volume23
Issue number2
DOIs
StatePublished - 1985

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Selenocysteine
Tissue Distribution
Selenium
Rats
Tissue
Proteins
Glutathione Peroxidase
Selenious Acid
Animals
Radioactivity
Liver
Peroxidase

ASJC Scopus subject areas

  • Biochemistry
  • Inorganic Chemistry

Cite this

Abundance and tissue distribution of selenocysteine-containing proteins in the rat. / Hawkes, Wayne C.; Wilhelmsen, Eric C.; Tappel, Al L.

In: Journal of Inorganic Biochemistry, Vol. 23, No. 2, 1985, p. 77-92.

Research output: Contribution to journalArticle

Hawkes, Wayne C. ; Wilhelmsen, Eric C. ; Tappel, Al L. / Abundance and tissue distribution of selenocysteine-containing proteins in the rat. In: Journal of Inorganic Biochemistry. 1985 ; Vol. 23, No. 2. pp. 77-92.
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