Absorption of biliary cobalamin in baboons following total gastrectomy

Ralph Green, D. W. Jacobsen, S. V. van Tonder, M. C. Kew, J. Metz

Research output: Contribution to journalArticle

14 Scopus citations

Abstract

Absorption of radiolabeled cobalamin in baboons was assessed by whole body counting. Retention of biliary cobalamin and an aqueous solution of cyanocobalamin was measured in normal baboons and in baboons after total gastrectomy by using 57Co-labeled biliary cobalamin and 58Co-cyanocobalamin, with and without baboon gastric juice containing intrinsic factor. Radiolabeled biliary cobalamin was obtained by intravenous injection of 57Co-cyanocobalamin in baboons and collection of bile through a cannula placed in the common bile duct. Cobalamin absorption was not completely abolished by gastrectomy and biliary cobalamin was better retained than cyanocobalamin; intrinsic factor enhanced absorption of both forms. After gastrectomy there was steady depletion of liver and serum cobalamin levels, which ceased after a new equilibrium was reached between a progressively diminishing cobalamin loss and the impaired but significant residual level of absorption. These studies in the nonhuman primate provide further information concerning the enterohepatic circulation of cobalamin and suggest that the form of cobalamin in bile may be more readily absorbed than is cyanocobalamin or that bile itself may have an enhancing effect on cobalamin absorption. The data also suggest that physiologically significant amounts of cobalamin may be absorbed in the absence of a gastric source of intrinsic factor.

Original languageEnglish (US)
Pages (from-to)771-777
Number of pages7
JournalJournal of Laboratory and Clinical Medicine
Volume100
Issue number5
StatePublished - 1982
Externally publishedYes

ASJC Scopus subject areas

  • Medicine(all)
  • Pathology and Forensic Medicine

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    Green, R., Jacobsen, D. W., van Tonder, S. V., Kew, M. C., & Metz, J. (1982). Absorption of biliary cobalamin in baboons following total gastrectomy. Journal of Laboratory and Clinical Medicine, 100(5), 771-777.