Absence of the RGS9·Gβ5 GTPase-activating Complex in Photoreceptors of the R9AP Knockout Mouse

Gabor Keresztes, Kirill A. Martemyanov, Claudia M. Krispel, Hideki Mutai, Peter J. Yoo, Stephane F. Maison, Marie E Burns, Vadim Y. Arshavsky, Stefan Heller

Research output: Contribution to journalArticle

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Abstract

Timely termination of the light response in retinal photoreceptors requires rapid inactivation of the G protein transducin. This is achieved through the stimulation of transducin GTPase activity by the complex of the ninth member of the regulator of G protein signaling protein family (RGS9) with type 5 G protein β subunit (Gβ5). RGS9·Gβ5 is anchored to photoreceptor disc membranes by the transmembrane protein, R9AP. In this study, we analyzed visual signaling in the rods of R9AP knockout mice. We found that light responses from R9AP knockout rods were very slow to recover and were indistinguishable from those of RGS9 or Gβ5 knockout rods. This effect was a consequence of the complete absence of any detectable RGS9 from the retinas of R9AP knockout mice. On the other hand, the level of RGS9 mRNA was not affected by the knockout. These data indicate that in photoreceptors R9AP determines the stability of the RGS9·Gβ5 complex, and therefore all three proteins, RGS9, Gβ5, and R9AP, are obligate members of the regulatory complex that speeds the rate at which transducin hydrolyzes GTP.

Original languageEnglish (US)
Pages (from-to)1581-1584
Number of pages4
JournalJournal of Biological Chemistry
Volume279
Issue number3
DOIs
StatePublished - Jan 16 2004

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Transducin
GTP Phosphohydrolases
GTP-Binding Proteins
Knockout Mice
RGS Proteins
Light
Vertebrate Photoreceptor Cells
Protein Subunits
Guanosine Triphosphate
Retina
Membrane Proteins
Proteins
Membranes
Messenger RNA
transducin GTP phosphohydrolase

ASJC Scopus subject areas

  • Biochemistry

Cite this

Keresztes, G., Martemyanov, K. A., Krispel, C. M., Mutai, H., Yoo, P. J., Maison, S. F., ... Heller, S. (2004). Absence of the RGS9·Gβ5 GTPase-activating Complex in Photoreceptors of the R9AP Knockout Mouse. Journal of Biological Chemistry, 279(3), 1581-1584. https://doi.org/10.1074/jbc.C300456200

Absence of the RGS9·Gβ5 GTPase-activating Complex in Photoreceptors of the R9AP Knockout Mouse. / Keresztes, Gabor; Martemyanov, Kirill A.; Krispel, Claudia M.; Mutai, Hideki; Yoo, Peter J.; Maison, Stephane F.; Burns, Marie E; Arshavsky, Vadim Y.; Heller, Stefan.

In: Journal of Biological Chemistry, Vol. 279, No. 3, 16.01.2004, p. 1581-1584.

Research output: Contribution to journalArticle

Keresztes, G, Martemyanov, KA, Krispel, CM, Mutai, H, Yoo, PJ, Maison, SF, Burns, ME, Arshavsky, VY & Heller, S 2004, 'Absence of the RGS9·Gβ5 GTPase-activating Complex in Photoreceptors of the R9AP Knockout Mouse', Journal of Biological Chemistry, vol. 279, no. 3, pp. 1581-1584. https://doi.org/10.1074/jbc.C300456200
Keresztes, Gabor ; Martemyanov, Kirill A. ; Krispel, Claudia M. ; Mutai, Hideki ; Yoo, Peter J. ; Maison, Stephane F. ; Burns, Marie E ; Arshavsky, Vadim Y. ; Heller, Stefan. / Absence of the RGS9·Gβ5 GTPase-activating Complex in Photoreceptors of the R9AP Knockout Mouse. In: Journal of Biological Chemistry. 2004 ; Vol. 279, No. 3. pp. 1581-1584.
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