Absence of synaptic regulation by phosducin in retinal slices

James H. Long, Vadim Y. Arshavsky, Marie E Burns

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Phosducin is an abundant photoreceptor protein that binds G-protein βγ subunits and plays a role in modulating synaptic transmission at photoreceptor synapses under both dark-adapted and light-adapted conditions in vivo. To examine the role of phosducin at the rod-to-rod bipolar cell (RBC) synapse, we used whole-cell voltage clamp recordings to measure the light-evoked currents from both wild-type (WT) and phosducin knockout (Pd-/-) RBCs, in dark- and light-adapted retinal slices. Pd-/- RBCs showed smaller dim flash responses and steeper intensity-response relationships than WT RBCs, consistent with the smaller rod responses being selectively filtered out by the non-linear threshold at the rod-to-rod bipolar synapse. In addition, Pd-/- RBCs showed a marked delay in the onset of the light-evoked currents, similar to that of a WT response to an effectively dimmer flash. Comparison of the changes in flash sensitivity in the presence of steady adapting light revealed that Pd-/- RBCs desensitized less than WT RBCs to the same intensity. These results are quantitatively consistent with the smaller single photon responses of Pd-/- rods, owing to the known reduction in rod G-protein expression levels in this line. The absence of an additional synaptic phenotype in these experiments suggests that the function of phosducin at the photoreceptor synapse is abolished by the conditions of retinal slice recordings.

Original languageEnglish (US)
Article numbere83970
JournalPLoS One
Volume8
Issue number12
DOIs
StatePublished - Dec 20 2013

Fingerprint

rods (retina)
Synapses
erythrocytes
Light
synapse
photoreceptors
GTP-Binding Proteins
G-proteins
Clamping devices
Protein Subunits
Photons
Synaptic Transmission
synaptic transmission
protein subunits
phosducin
Phenotype
protein synthesis
Electric potential
cells
phenotype

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

Absence of synaptic regulation by phosducin in retinal slices. / Long, James H.; Arshavsky, Vadim Y.; Burns, Marie E.

In: PLoS One, Vol. 8, No. 12, e83970, 20.12.2013.

Research output: Contribution to journalArticle

Long, James H. ; Arshavsky, Vadim Y. ; Burns, Marie E. / Absence of synaptic regulation by phosducin in retinal slices. In: PLoS One. 2013 ; Vol. 8, No. 12.
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