Absence of kdr resistance alleles in the Union of the Comoros, East Africa

Yoosook Lee, Natalie Olson, Youki Yamasaki, Allison Chang, Clare Marsden, Ahmed Ouledi, Gregory C Lanzaro, Anthony Cornel

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Knockdown resistance (kdr) and CYP9K1 genotypes were detected by a MOLDI-TOF based SNP genotyping assay (Sequenom iPLEX) in samples of Anopheles gambiae collected at 13 sites throughout the Union of the Comoros and Dar es Salaam, Tanzania during February and March 2011. All A. gambiae specimens collected in the Comoros were homozygous for the susceptible kdr alleles (+/+) while 96% of A. gambiae from Dar es Salaam were homozygous for the East African kdr resistant genotype (E/E). In contrast, all specimens from Dar es Salaam and the Comoros were homozygous for the cyp3 allele (c3/c3) at the CYP9K1 locus; the locus has been implicated in metabolic resistance against pyrethroid insecticides in West Africa. All specimens had typical A. gambiae genotypes for SNPs within the divergence Islands on all three chromosomes. Although further spatial and temporal studies are needed, the distribution of kdr genotypes between the Comoros and Tanzania further supports isolation of the Comoros populations from A. gambiae populations on mainland Africa.

Original languageEnglish (US)
Article number146
JournalF1000Research
Volume4
DOIs
StatePublished - Jun 9 2015

Fingerprint

Comoros
Anopheles gambiae
Eastern Africa
Pyrethrins
Chromosomes
Insecticides
Assays
Alleles
Genotype
Tanzania
Single Nucleotide Polymorphism
Western Africa
Time and Motion Studies
Islands
Population

ASJC Scopus subject areas

  • Medicine(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Immunology and Microbiology(all)
  • Pharmacology, Toxicology and Pharmaceutics(all)

Cite this

Lee, Y., Olson, N., Yamasaki, Y., Chang, A., Marsden, C., Ouledi, A., ... Cornel, A. (2015). Absence of kdr resistance alleles in the Union of the Comoros, East Africa. F1000Research, 4, [146]. https://doi.org/10.12688/f1000research.6567.1

Absence of kdr resistance alleles in the Union of the Comoros, East Africa. / Lee, Yoosook; Olson, Natalie; Yamasaki, Youki; Chang, Allison; Marsden, Clare; Ouledi, Ahmed; Lanzaro, Gregory C; Cornel, Anthony.

In: F1000Research, Vol. 4, 146, 09.06.2015.

Research output: Contribution to journalArticle

Lee, Yoosook ; Olson, Natalie ; Yamasaki, Youki ; Chang, Allison ; Marsden, Clare ; Ouledi, Ahmed ; Lanzaro, Gregory C ; Cornel, Anthony. / Absence of kdr resistance alleles in the Union of the Comoros, East Africa. In: F1000Research. 2015 ; Vol. 4.
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