TY - JOUR
T1 - Absence of galectin-3 does not affect the development of experimental tongue carcinomas in mice
AU - de Faria, Paulo Rogério
AU - Chammas, Roger
AU - de Melo, Thaissa Lopes
AU - Hsu, Daniel K.
AU - Liu, Fu-Tong
AU - Nonogaki, Suely
AU - Cardoso, Sérgio Vitorino
AU - Loyola, Adriano Mota
PY - 2011/4
Y1 - 2011/4
N2 - Background: Galectin-3 is a lectin that presents pivotal roles in tumor biology and there are no studies evaluating their expression in dysplasias and carcinomas developed from tongue carcinogenesis models. Aims: To investigate the role of galectin-3 in the development of tongue carcinomas using a mouse model of oral carcinogenesis. Methods: Galectin-3-deficient (gal3-/-) and wild-type (gal3+/+) mice were challenged with 4-nitroquinoline-1-oxide in drinking water for 16weeks and killed at different times. Tongues were removed and the number of dysplasias and carcinomas was counted. An immunohistochemical study for galectin-3 was performed only in the tongue from gal3+/+ mice. Results: In both groups, a reduction of dysplasias and an increase of carcinomas from week 16 to week 32 (p > 0.05) were observed. A predominance of high cytoplasmic and nuclear galectin-3 expression was observed in carcinomas (64.7%) and dysplasias (55.5%), respectively (p > 0.05). The perilesional areas always presented a statistical cytoplasmic and nuclear galectin-3 overexpression. Conclusions: Absence of galectin-3 did not directly affect the process of carcinogenesis and a cytoplasm shift of galectin-3 seems to be associated with development of tongue carcinomas.
AB - Background: Galectin-3 is a lectin that presents pivotal roles in tumor biology and there are no studies evaluating their expression in dysplasias and carcinomas developed from tongue carcinogenesis models. Aims: To investigate the role of galectin-3 in the development of tongue carcinomas using a mouse model of oral carcinogenesis. Methods: Galectin-3-deficient (gal3-/-) and wild-type (gal3+/+) mice were challenged with 4-nitroquinoline-1-oxide in drinking water for 16weeks and killed at different times. Tongues were removed and the number of dysplasias and carcinomas was counted. An immunohistochemical study for galectin-3 was performed only in the tongue from gal3+/+ mice. Results: In both groups, a reduction of dysplasias and an increase of carcinomas from week 16 to week 32 (p > 0.05) were observed. A predominance of high cytoplasmic and nuclear galectin-3 expression was observed in carcinomas (64.7%) and dysplasias (55.5%), respectively (p > 0.05). The perilesional areas always presented a statistical cytoplasmic and nuclear galectin-3 overexpression. Conclusions: Absence of galectin-3 did not directly affect the process of carcinogenesis and a cytoplasm shift of galectin-3 seems to be associated with development of tongue carcinomas.
KW - Galectin-3
KW - Mice
KW - Oral carcinogenesis
KW - Oral squamous cell carcinoma
KW - Premalignant lesions
KW - Tongue
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U2 - 10.1016/j.yexmp.2010.12.007
DO - 10.1016/j.yexmp.2010.12.007
M3 - Article
C2 - 21194530
AN - SCOPUS:78651231163
VL - 90
SP - 189
EP - 193
JO - Experimental and Molecular Pathology
JF - Experimental and Molecular Pathology
SN - 0014-4800
IS - 2
ER -