Absence of bovine leukosis virus in semen of seropositive bulls

K. Yeon Choi, Don Monke, Jeffrey L Stott

Research output: Contribution to journalArticle

11 Scopus citations

Abstract

A polymerase chain reaction (PCR)-based detection system was established to identify the presence of bovine leukosis virus (BLV) DNA in bovine semen. Seventy-nine bulls were included in the study. Serum, peripheral blood leukocytes, and semen were collected from each of the 79 bulls. The BLV-specific antibody was detected in serum by agar gel immunodiffusion and viral DNA in blood and semen by PCR. Serologically, 29 of the 79 bulls were BLV positive. Twenty-seven of the 29 seropositive bulls and 1 of the seronegative bulls had BLV DNA in peripheral blood leukocytes. All 79 bulls tested PCR negative for the presence of BLV in semen. This data is strong evidence that properly collected semen from BLV seropositive bulls will not contribute to dissemination of this viral infection.

Original languageEnglish (US)
Pages (from-to)403-406
Number of pages4
JournalJournal of Veterinary Diagnostic Investigation
Volume14
Issue number5
StatePublished - Sep 2002

ASJC Scopus subject areas

  • Microbiology
  • veterinary(all)

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