Abnormalities of the FHIT transcripts in osteosarcoma and Ewing sarcoma

Shin Ichi Hinohara, Noriko Satake, Ki Ichi Sekine, Yasuhiko Kaneko

Research output: Contribution to journalArticle

11 Scopus citations

Abstract

In a study of FHIT gene abnormalities by reverse transcription-polymerase chain reaction (RT-PCR) and sequence analysis of the PCR products, we found normal and abnormal PCR products in 11 osteosarcomas, one osteosarcoma cell line and 3 Ewing sarcomas, and a normal PCR product only in 5 osteosarcomas and 8 Ewing sarcomas. Sequence analysis of the abnormal PCR products revealed 7 osteosarcomas lacking exons 4 to 6, 7, 8 or 9, two lacking exons 5 to 7 or 10, and two lacking exons 6 to 8 or 10. In the aberrant transcripts of the 11 osteosarcomas, fusion had occurred in the exon/intron junctions in 2 tumors, between a segment within an exon and a complete exon in 3, and between segments within exons in 6. The 3 Ewing sarcomas had lost exon 4 or 5 to exon 6 or 10, and fusion had occurred in the exon/intron junction in one, and between segments within exons in 2. These findings suggest that both abnormal or variant splicing and other mechanisms such as genomic instabilities in the FHIT locus may have resulted in the expression of aberrant transcripts. One osteosarcoma and one cell line established from this osteosarcoma showed different abnormal FHIT transcripts, indicating that the tumor cells with the initial aberrant transcripts may not have had a selective advantage for proliferation. The FHIT abnormalities did not seem to be correlated with lung metastasis or a poor clinical outcome in our patients with osteosarcoma or Ewing sarcoma.

Original languageEnglish (US)
Pages (from-to)887-894
Number of pages8
JournalJapanese Journal of Cancer Research
Volume89
Issue number9
StatePublished - Sep 1998
Externally publishedYes

Keywords

  • Ewing sarcoma
  • FHIT
  • Osteosarcoma

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

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