A yeast protein, homologous to the proteolipid of the chromaffin granule proton-ATPase, is important for cell growth

Michelle L Apperson, Robert E. Jensen, Kitaru Suda, Christine Witte, Michael P. Yaffe

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

We have characterized a gene, PPA1, adjacent to the yeast MAS2 gene. DNA sequence analysis of PPA1 predicts a hydrophobic protein of 23 kDa. This protein is homologous to the proteolipid of the bovine chromaffin granule proton ATPase and to the proteolipid of the yeast vacuolar proton ATPase. Gene disruption experiments indicate that the PPA1 protein is essential for viability in three unrelated yeast strains and important for optimal growth in a fourth strain.

Original languageEnglish (US)
Pages (from-to)574-579
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume168
Issue number2
DOIs
StatePublished - Apr 30 1990

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Chromaffin Granules
Proteolipids
Fungal Proteins
Cell growth
Yeast
Adenosine Triphosphatases
Protons
Genes
Yeasts
Growth
Vacuolar Proton-Translocating ATPases
Proteins
DNA sequences
DNA Sequence Analysis
Experiments

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

A yeast protein, homologous to the proteolipid of the chromaffin granule proton-ATPase, is important for cell growth. / Apperson, Michelle L; Jensen, Robert E.; Suda, Kitaru; Witte, Christine; Yaffe, Michael P.

In: Biochemical and Biophysical Research Communications, Vol. 168, No. 2, 30.04.1990, p. 574-579.

Research output: Contribution to journalArticle

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