A transcript map encompassing the multiple endocrine neoplasia type-1 (MEN1) locus on chromosome 11q13

Mark Sawicki, Elizabeth Arnold, Sam Ebrahimi, Thomas Duell, Jin Sang, Thomas Wood, Rita Chakrabarti, Julius Peters, Yu-Jui Yvonne Wan, Ghasan Samara, Heinz Ulrich G Weier, Niton Udar, Edward Passaro, Eri S. Srivatsan

Research output: Contribution to journalArticlepeer-review

11 Scopus citations

Abstract

A transcription map of a 1200-kb region encompassing the MEN1 locus was constructed by direct cDNA selection and mapping ESTs. A total of 29 genes were mapped. Ten transcripts were identified by cDNA selection of a focused 300-kb genomic region telomeric to the MEN1 consensus region. Since many of the sequences cloned by cDNA selection also identified ESTs from the region, 19 additional RH-mapped ESTs were mapped to the entire contig region by PCR amplification of genomic clones. Nine known genes, 2 putative human homologues to mouse genes, and 18 novel transcripts map to the region. Transcripts that map to the MEN1 interval PYGM-D11S449 include SGC35223, IB1256, AA147620, ZFM1, FAU, and CAPN1. The latter a known genes have already been excluded as candidate MEN1 genes. The 2 putative human homologues of mouse genes Ltbp2 and Spa-1 may be candidate tumor suppressor genes, but they map telomeric to D11S449. Although both of these genes map outside the MEN1 consensus region they may play a role in sporadic endocrine tumors independent of the MEN1 gene or in other tumors, such as breast cancer, that have loss of heterozygosity within this region.

Original languageEnglish (US)
Pages (from-to)405-412
Number of pages8
JournalGenomics
Volume42
Issue number3
DOIs
StatePublished - Jun 15 1997

ASJC Scopus subject areas

  • Genetics

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