Abstract
Saccharomyces cerevisiae glutamine tRNA(CAG) is encoded by an intronless, single-copy gene, SUP60. We have imposed a requirement for splicing in the biosynthesis of this tRNA by inserting a synthetic intron in the SUP60 gene. Genetic analysis demonstrated that the interrupted gene produces a functional, mature tRNA product in vivo.
Original language | English (US) |
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Pages (from-to) | 329-331 |
Number of pages | 3 |
Journal | Molecular and Cellular Biology |
Volume | 9 |
Issue number | 1 |
DOIs | |
State | Published - Jan 1 1989 |
Externally published | Yes |
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ASJC Scopus subject areas
- Molecular Biology
- Cell Biology
Cite this
A synthetic intron in a naturally intronless yeast pre-tRNA is spliced efficiently in vivo. / Winey, Mark; Edelman, I.; Culbertson, M. R.
In: Molecular and Cellular Biology, Vol. 9, No. 1, 01.01.1989, p. 329-331.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - A synthetic intron in a naturally intronless yeast pre-tRNA is spliced efficiently in vivo.
AU - Winey, Mark
AU - Edelman, I.
AU - Culbertson, M. R.
PY - 1989/1/1
Y1 - 1989/1/1
N2 - Saccharomyces cerevisiae glutamine tRNA(CAG) is encoded by an intronless, single-copy gene, SUP60. We have imposed a requirement for splicing in the biosynthesis of this tRNA by inserting a synthetic intron in the SUP60 gene. Genetic analysis demonstrated that the interrupted gene produces a functional, mature tRNA product in vivo.
AB - Saccharomyces cerevisiae glutamine tRNA(CAG) is encoded by an intronless, single-copy gene, SUP60. We have imposed a requirement for splicing in the biosynthesis of this tRNA by inserting a synthetic intron in the SUP60 gene. Genetic analysis demonstrated that the interrupted gene produces a functional, mature tRNA product in vivo.
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UR - http://www.scopus.com/inward/citedby.url?scp=0024486017&partnerID=8YFLogxK
U2 - 10.1128/MCB.9.1.329
DO - 10.1128/MCB.9.1.329
M3 - Article
C2 - 2648132
AN - SCOPUS:0024486017
VL - 9
SP - 329
EP - 331
JO - Molecular and Cellular Biology
JF - Molecular and Cellular Biology
SN - 0270-7306
IS - 1
ER -