Abstract
Saccharomyces cerevisiae glutamine tRNA(CAG) is encoded by an intronless, single-copy gene, SUP60. We have imposed a requirement for splicing in the biosynthesis of this tRNA by inserting a synthetic intron in the SUP60 gene. Genetic analysis demonstrated that the interrupted gene produces a functional, mature tRNA product in vivo.
Original language | English (US) |
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Pages (from-to) | 329-331 |
Number of pages | 3 |
Journal | Molecular and Cellular Biology |
Volume | 9 |
Issue number | 1 |
DOIs | |
State | Published - Jan 1 1989 |
Externally published | Yes |
ASJC Scopus subject areas
- Molecular Biology
- Cell Biology