A synthetic intron in a naturally intronless yeast pre-tRNA is spliced efficiently in vivo.

Mark Winey, I. Edelman, M. R. Culbertson

Research output: Contribution to journalArticle

3 Scopus citations

Abstract

Saccharomyces cerevisiae glutamine tRNA(CAG) is encoded by an intronless, single-copy gene, SUP60. We have imposed a requirement for splicing in the biosynthesis of this tRNA by inserting a synthetic intron in the SUP60 gene. Genetic analysis demonstrated that the interrupted gene produces a functional, mature tRNA product in vivo.

Original languageEnglish (US)
Pages (from-to)329-331
Number of pages3
JournalMolecular and Cellular Biology
Volume9
Issue number1
DOIs
StatePublished - Jan 1 1989
Externally publishedYes

ASJC Scopus subject areas

  • Molecular Biology
  • Cell Biology

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