A synthetic intron in a naturally intronless yeast pre-tRNA is spliced efficiently in vivo.

Mark Winey; I. Edelman; M. R. Culbertson

doi:10.1128/MCB.9.1.329

A synthetic intron in a naturally intronless yeast pre-tRNA is spliced efficiently in vivo.

Mark Winey, I. Edelman, M. R. Culbertson

Research output: Contribution to journal › Article › peer-review

3 Scopus citations

Abstract

Saccharomyces cerevisiae glutamine tRNA(CAG) is encoded by an intronless, single-copy gene, SUP60. We have imposed a requirement for splicing in the biosynthesis of this tRNA by inserting a synthetic intron in the SUP60 gene. Genetic analysis demonstrated that the interrupted gene produces a functional, mature tRNA product in vivo.

Original language	English (US)
Pages (from-to)	329-331
Number of pages	3
Journal	Molecular and Cellular Biology
Volume	9
Issue number	1
DOIs	https://doi.org/10.1128/MCB.9.1.329
State	Published - Jan 1 1989
Externally published	Yes

ASJC Scopus subject areas

Molecular Biology
Cell Biology

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10.1128/MCB.9.1.329

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title = "A synthetic intron in a naturally intronless yeast pre-tRNA is spliced efficiently in vivo.",

abstract = "Saccharomyces cerevisiae glutamine tRNA(CAG) is encoded by an intronless, single-copy gene, SUP60. We have imposed a requirement for splicing in the biosynthesis of this tRNA by inserting a synthetic intron in the SUP60 gene. Genetic analysis demonstrated that the interrupted gene produces a functional, mature tRNA product in vivo.",

author = "Mark Winey and I. Edelman and Culbertson, {M. R.}",

year = "1989",

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AU - Edelman, I.

AU - Culbertson, M. R.

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N2 - Saccharomyces cerevisiae glutamine tRNA(CAG) is encoded by an intronless, single-copy gene, SUP60. We have imposed a requirement for splicing in the biosynthesis of this tRNA by inserting a synthetic intron in the SUP60 gene. Genetic analysis demonstrated that the interrupted gene produces a functional, mature tRNA product in vivo.

AB - Saccharomyces cerevisiae glutamine tRNA(CAG) is encoded by an intronless, single-copy gene, SUP60. We have imposed a requirement for splicing in the biosynthesis of this tRNA by inserting a synthetic intron in the SUP60 gene. Genetic analysis demonstrated that the interrupted gene produces a functional, mature tRNA product in vivo.

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A synthetic intron in a naturally intronless yeast pre-tRNA is spliced efficiently in vivo.

Abstract

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