Abstract
Saccharomyces cerevisiae glutamine tRNA(CAG) is encoded by an intronless, single-copy gene, SUP60. We have imposed a requirement for splicing in the biosynthesis of this tRNA by inserting a synthetic intron in the SUP60 gene. Genetic analysis demonstrated that the interrupted gene produces a functional, mature tRNA product in vivo.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 329-331 |
| Number of pages | 3 |
| Journal | Molecular and Cellular Biology |
| Volume | 9 |
| Issue number | 1 |
| DOIs | |
| State | Published - Jan 1 1989 |
| Externally published | Yes |
ASJC Scopus subject areas
- Molecular Biology
- Cell Biology
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Cite this
Winey, M., Edelman, I., & Culbertson, M. R. (1989). A synthetic intron in a naturally intronless yeast pre-tRNA is spliced efficiently in vivo. Molecular and Cellular Biology, 9(1), 329-331. https://doi.org/10.1128/MCB.9.1.329