A synthetic intron in a naturally intronless yeast pre-tRNA is spliced efficiently in vivo.

Mark Winey, I. Edelman, M. R. Culbertson

Research output: Contribution to journal › Article

3 Citations (Scopus)

Abstract

Saccharomyces cerevisiae glutamine tRNA(CAG) is encoded by an intronless, single-copy gene, SUP60. We have imposed a requirement for splicing in the biosynthesis of this tRNA by inserting a synthetic intron in the SUP60 gene. Genetic analysis demonstrated that the interrupted gene produces a functional, mature tRNA product in vivo.

Original languageEnglish (US)
Pages (from-to)329-331
Number of pages3
JournalMolecular and Cellular Biology
Volume9
Issue number1
DOIs
StatePublished - Jan 1 1989
Externally publishedYes

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RNA Precursors
Transfer RNA
Introns
Yeasts
Genes
Glutamine
Saccharomyces cerevisiae

ASJC Scopus subject areas

  • Molecular Biology
  • Cell Biology

Cite this

A synthetic intron in a naturally intronless yeast pre-tRNA is spliced efficiently in vivo. / Winey, Mark; Edelman, I.; Culbertson, M. R.

In: Molecular and Cellular Biology, Vol. 9, No. 1, 01.01.1989, p. 329-331.

Research output: Contribution to journal › Article

Winey, M, Edelman, I & Culbertson, MR 1989, 'A synthetic intron in a naturally intronless yeast pre-tRNA is spliced efficiently in vivo.', Molecular and Cellular Biology, vol. 9, no. 1, pp. 329-331. https://doi.org/10.1128/MCB.9.1.329
Winey M, Edelman I, Culbertson MR. A synthetic intron in a naturally intronless yeast pre-tRNA is spliced efficiently in vivo. Molecular and Cellular Biology. 1989 Jan 1;9(1):329-331. https://doi.org/10.1128/MCB.9.1.329
Winey, Mark ; Edelman, I. ; Culbertson, M. R. / A synthetic intron in a naturally intronless yeast pre-tRNA is spliced efficiently in vivo. In: Molecular and Cellular Biology. 1989 ; Vol. 9, No. 1. pp. 329-331.
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