A synteny map of the horse genome comprised of 240 microsatellite and RAPD markers

Y. L. Shiue, L. A. Bickel, A. R. Caetano, L. V. Millon, R. S. Clark, M. L. Eggleston, A. T. Bowling, Richard W Michelmore, E. Bailey, G. Guérin, S. Godard, J. R. Mickelson, S. J. Valberg, J. D. Murray

Research output: Contribution to journalArticle

66 Citations (Scopus)

Abstract

To generate a domestic horse genome map we integrated synteny information for markers screened on a somatic cell hybrid (SCH) panel with published information for markers physically assigned to chromosomes. The mouse-horse SCH panel was established by fusing pSV2neo transformed primary horse fibroblasts to either RAG or LMTk mouse cells, followed by G418 antibiotic selection. For each of the 108 cell lines of the panel, we defined the presence or absence of 240 genetic markers by PCR, including 58 random amplified polymorphic DNA (RAPD) markers and 182 microsatellites. Thirty-three syntenic groups were defined, comprised of two to 26 markers with correlation coefficient (r) values ranging from 0.70 to 1.0. Based on significant correlation values with physically mapped microsatellite (type II) or gene (type I) markers, 22 syntenic groups were assigned to horse chromosomes (1, 2, 3, 4, 6, 9, 10, 11, 12, 13, 15, 18, 19, 20, 21, 22, 23, 24, 26, 30, X and Y). The other 11 syntenic groups were provisionally assigned to the remaining chromosomes based on information provided by heterologous species painting probes and work in progress with type I markers.

Original languageEnglish (US)
Pages (from-to)1-9
Number of pages9
JournalAnimal Genetics
Volume30
Issue number1
DOIs
StatePublished - Feb 1999

Fingerprint

Synteny
random amplified polymorphic DNA technique
Genetic Markers
Microsatellite Repeats
Horses
Genome
microsatellite repeats
horses
genetic markers
genome
Hybrid Cells
chromosomes
somatic cells
Chromosomes
geneticin
Paintings
Chromosomes, Human, Pair 2
Chromosomes, Human, Pair 1
mice
fibroblasts

Keywords

  • Comparative gene mapping
  • Horse genetics
  • Horse genome map
  • Synteny map

ASJC Scopus subject areas

  • Animal Science and Zoology
  • Genetics
  • Genetics(clinical)

Cite this

Shiue, Y. L., Bickel, L. A., Caetano, A. R., Millon, L. V., Clark, R. S., Eggleston, M. L., ... Murray, J. D. (1999). A synteny map of the horse genome comprised of 240 microsatellite and RAPD markers. Animal Genetics, 30(1), 1-9. https://doi.org/10.1046/j.1365-2052.1999.00377.x

A synteny map of the horse genome comprised of 240 microsatellite and RAPD markers. / Shiue, Y. L.; Bickel, L. A.; Caetano, A. R.; Millon, L. V.; Clark, R. S.; Eggleston, M. L.; Bowling, A. T.; Michelmore, Richard W; Bailey, E.; Guérin, G.; Godard, S.; Mickelson, J. R.; Valberg, S. J.; Murray, J. D.

In: Animal Genetics, Vol. 30, No. 1, 02.1999, p. 1-9.

Research output: Contribution to journalArticle

Shiue, YL, Bickel, LA, Caetano, AR, Millon, LV, Clark, RS, Eggleston, ML, Bowling, AT, Michelmore, RW, Bailey, E, Guérin, G, Godard, S, Mickelson, JR, Valberg, SJ & Murray, JD 1999, 'A synteny map of the horse genome comprised of 240 microsatellite and RAPD markers', Animal Genetics, vol. 30, no. 1, pp. 1-9. https://doi.org/10.1046/j.1365-2052.1999.00377.x
Shiue YL, Bickel LA, Caetano AR, Millon LV, Clark RS, Eggleston ML et al. A synteny map of the horse genome comprised of 240 microsatellite and RAPD markers. Animal Genetics. 1999 Feb;30(1):1-9. https://doi.org/10.1046/j.1365-2052.1999.00377.x
Shiue, Y. L. ; Bickel, L. A. ; Caetano, A. R. ; Millon, L. V. ; Clark, R. S. ; Eggleston, M. L. ; Bowling, A. T. ; Michelmore, Richard W ; Bailey, E. ; Guérin, G. ; Godard, S. ; Mickelson, J. R. ; Valberg, S. J. ; Murray, J. D. / A synteny map of the horse genome comprised of 240 microsatellite and RAPD markers. In: Animal Genetics. 1999 ; Vol. 30, No. 1. pp. 1-9.
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