A 1H NMR technique for observing metabolite signals in the spectrum of perfused liver

Thomas Jue, F. Arias-Mendonza, N. C. Gonnella

Research output: Contribution to journalArticle

46 Scopus citations

Abstract

We have developed a 1H NMR technique to selectively edit the spectrum of perfused liver for specific resonances of metabolites that occur in low concentration. The method employs selective DANTE pulses, which avoid exciting the water signal and at the sme time control the J modulation effect in the homonuclear spin-echo experiment. By difference spectroscopy, we have suppressed the background signals from lipids and water and have resolved the CH3 resonance of lactate at 1.33 ppm. .Moreover, the technique is highly selective and allows us to select the CH3 resonance of alanine at 1.47 ppm in the presence of the CH3 resonance of lactate at 1.33 ppm, even though the latter was much larger before editing. We have applied this technique to study the metabolic effect of ethanol in perfused mouse liver and have observed that the rate of formation of lactate from pyruvate is increased by a factor of 2.8 when ethanol is added.

Original languageEnglish (US)
Pages (from-to)5246-5249
Number of pages4
JournalProceedings of the National Academy of Sciences of the United States of America
Volume82
Issue number16
StatePublished - 1985
Externally publishedYes

ASJC Scopus subject areas

  • General
  • Genetics

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