A SUMO-ubiquitin relay recruits proteasomes to chromosome axes to regulate meiotic recombination

H. B D Prasada Rao, Huanyu Qiao, Shubhang K. Bhatt, Logan R J Bailey, Hung D. Tran, Sarah L. Bourne, Wendy Qiu, Anusha Deshpande, Ajay N. Sharma, Connor J. Beebout, Roberto J. Pezza, Neil Hunter

Research output: Contribution to journalArticle

37 Citations (Scopus)

Abstract

Meiosis produces haploid gametes through a succession of chromosomal events including pairing, synapsis, and recombination. Mechanisms that orchestrate these events remain poorly understood. We found that the SUMO-modification and ubiquitin-proteasome systems regulate the major events of meiotic prophase in mouse. Interdependent localization of SUMO, ubiquitin and proteasomes along chromosome axes was mediated largely by RNF212 and HEI10, two E3 ligases that are also essential for crossover recombination. RNF212-dependent SUMO conjugation effected a checkpoint-like process that stalls recombination by rendering the turnover of a subset of recombination factors dependent on HEI10- mediated ubiquitylation. We propose that SUMO conjugation establishes a precondition for designating crossover sites via selective protein stabilization. Thus, meiotic chromosome axes are hubs for regulated proteolysis via SUMO-dependent control of the ubiquitin-proteasome system

Original languageEnglish (US)
Pages (from-to)1-10
Number of pages10
JournalScience
DOIs
StateAccepted/In press - Jan 5 2017

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Proteasome Endopeptidase Complex
Ubiquitin
Genetic Recombination
Chromosomes
Sumoylation
Chromosome Pairing
Prophase
Ubiquitin-Protein Ligases
Ubiquitination
Haploidy
Meiosis
Germ Cells
Proteolysis
Proteins

ASJC Scopus subject areas

  • Medicine(all)
  • General

Cite this

Rao, H. B. D. P., Qiao, H., Bhatt, S. K., Bailey, L. R. J., Tran, H. D., Bourne, S. L., ... Hunter, N. (Accepted/In press). A SUMO-ubiquitin relay recruits proteasomes to chromosome axes to regulate meiotic recombination. Science, 1-10. https://doi.org/10.1126/science.aaf6407

A SUMO-ubiquitin relay recruits proteasomes to chromosome axes to regulate meiotic recombination. / Rao, H. B D Prasada; Qiao, Huanyu; Bhatt, Shubhang K.; Bailey, Logan R J; Tran, Hung D.; Bourne, Sarah L.; Qiu, Wendy; Deshpande, Anusha; Sharma, Ajay N.; Beebout, Connor J.; Pezza, Roberto J.; Hunter, Neil.

In: Science, 05.01.2017, p. 1-10.

Research output: Contribution to journalArticle

Rao, HBDP, Qiao, H, Bhatt, SK, Bailey, LRJ, Tran, HD, Bourne, SL, Qiu, W, Deshpande, A, Sharma, AN, Beebout, CJ, Pezza, RJ & Hunter, N 2017, 'A SUMO-ubiquitin relay recruits proteasomes to chromosome axes to regulate meiotic recombination', Science, pp. 1-10. https://doi.org/10.1126/science.aaf6407
Rao, H. B D Prasada ; Qiao, Huanyu ; Bhatt, Shubhang K. ; Bailey, Logan R J ; Tran, Hung D. ; Bourne, Sarah L. ; Qiu, Wendy ; Deshpande, Anusha ; Sharma, Ajay N. ; Beebout, Connor J. ; Pezza, Roberto J. ; Hunter, Neil. / A SUMO-ubiquitin relay recruits proteasomes to chromosome axes to regulate meiotic recombination. In: Science. 2017 ; pp. 1-10.
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