A study of crystalline biomaterials for articular cartilage bioengineering

Talia Gross-Aviv, Bryan B. DiCarlo, Margaret M. French, Kyriacos A. Athanasiou, Razi Vago

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

This study examines the suitability of marine origin coral species, Porites lutea (POR) and the hydrozoan Millepora dichotoma (MIL), for use as novel three dimensional growth matrices in the field of articular cartilage tissue engineering. Therefore, mesenchymal stem cells (MSCs) and chondrocytes were grown on the skeletal material obtained from each of these two organisms to investigate their potential use as three dimensional scaffolding for cartilage tissue growth. Chondrogenic induction of MSCs was achieved by addition of transforming growth factor-β1 (TGF-β1) and insulin growth factor-I (IGF-I). Cell adherence, proliferation, differentiation and tissue development were investigated through six weeks of culture. Cartilage tissue growth and chondrocytic phenotype maintenance of each cell type were examined by cell morphology, histochemical analyses, expression of collagen type II and quantitative measures of glycosaminoglycan (GAG) content. The MSCs and the chondrocytes were shown good adherence to the scaffolds and maintenance of the chondrocytic phenotype in the initial stages of culture. However after two weeks of culture on MIL and three weeks on POR these cultures began to exhibit signs of further differentiation and phenotypic loss. The shown results indicated that POR was a better substrate for chondrocytes phenotype maintenance than MIL. We believe that surface modification of POR combined with mechanical stimuli will provide a suitable environment for chondrogenic phenotype maintenance. Further investigation of POR and other novel coralline biomatrices is indicated and warranted in the field of cartilage tissue engineering applications.

Original languageEnglish (US)
Pages (from-to)1388-1400
Number of pages13
JournalMaterials Science and Engineering C
Volume28
Issue number8
DOIs
StatePublished - Dec 1 2008
Externally publishedYes

Fingerprint

bioengineering
cartilage
Cartilage
Biocompatible Materials
phenotype
Biomaterials
Stem cells
maintenance
stem cells
Crystalline materials
Tissue
Tissue engineering
tissue engineering
cells
Collagen Type II
Insulin
Transforming Growth Factors
Glycosaminoglycans
insulin
Collagen

Keywords

  • Biomaterials
  • Chondrocytes
  • Coral
  • Hydrozoa
  • Mesenchymal stem cells (MSCs)
  • Tissue engineering

ASJC Scopus subject areas

  • Materials Science(all)
  • Condensed Matter Physics
  • Mechanical Engineering
  • Mechanics of Materials

Cite this

Gross-Aviv, T., DiCarlo, B. B., French, M. M., Athanasiou, K. A., & Vago, R. (2008). A study of crystalline biomaterials for articular cartilage bioengineering. Materials Science and Engineering C, 28(8), 1388-1400. https://doi.org/10.1016/j.msec.2008.03.006

A study of crystalline biomaterials for articular cartilage bioengineering. / Gross-Aviv, Talia; DiCarlo, Bryan B.; French, Margaret M.; Athanasiou, Kyriacos A.; Vago, Razi.

In: Materials Science and Engineering C, Vol. 28, No. 8, 01.12.2008, p. 1388-1400.

Research output: Contribution to journalArticle

Gross-Aviv, T, DiCarlo, BB, French, MM, Athanasiou, KA & Vago, R 2008, 'A study of crystalline biomaterials for articular cartilage bioengineering', Materials Science and Engineering C, vol. 28, no. 8, pp. 1388-1400. https://doi.org/10.1016/j.msec.2008.03.006
Gross-Aviv, Talia ; DiCarlo, Bryan B. ; French, Margaret M. ; Athanasiou, Kyriacos A. ; Vago, Razi. / A study of crystalline biomaterials for articular cartilage bioengineering. In: Materials Science and Engineering C. 2008 ; Vol. 28, No. 8. pp. 1388-1400.
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