A strategy for annotating the human milk glycome

Milady R. Ninonuevo, Youmie Park, Hongfeng Yin, Jinhua Zhang, Robert E. Ward, Brian H. Clowers, J. Bruce German, Samara L. Freeman, Kevin Killeen, Rudolf Grimm, Carlito B Lebrilla

Research output: Contribution to journalArticle

292 Citations (Scopus)

Abstract

Oligosaccharides in human milk represent a group of bioactive molecules that have evolved to be an abundant and diverse component of human milk, even though they have no direct nutritive value to the infant. A recent hypothesis proposes that they could be substrates for the development of the intestinal microflora and the mucosal immune system. The inability to determine the exact composition of these oligosaccharides limits research and the ability to understand their biological functions. Oligosaccharides isolated from the lipids and proteins of individual human milk samples were analyzed by a combination of techniques including microchip liquid chromatography mass spectrometry (HPLC-Chip/MS) and matrix-assisted laser desorption/ionization Fourier transform ion cyclotron resonance mass spectrometry (MALDI-FT ICR MS). Accurate mass measurements obtained using an orthogonal time-of-flight (o-TOF) mass spectrometry provided oligosaccharide composition for approximately 200 individual molecular species. Comparison of HPLC-Chip/MS profiles from five different women revealed variations in milk oligosaccharide compositions. HPLC-Chip/MS profiling provides a method for routinely identifying milk oligosaccharides. Tandem MS in combination with exoglycosidase digestion provides unambiguous differentiation of structural isomers.

Original languageEnglish (US)
Pages (from-to)7471-7480
Number of pages10
JournalJournal of Agricultural and Food Chemistry
Volume54
Issue number20
DOIs
StatePublished - Oct 4 2006

Fingerprint

Human Milk
breast milk
Oligosaccharides
oligosaccharides
Mass spectrometry
Mass Spectrometry
high performance liquid chromatography
High Pressure Liquid Chromatography
Milk
Chemical analysis
mass spectrometry
Cyclotrons
milk
Cyclotron resonance
matrix-assisted laser desorption-ionization mass spectrometry
glycosidases
Immune system
Glycoside Hydrolases
Matrix-Assisted Laser Desorption-Ionization Mass Spectrometry
Liquid chromatography

Keywords

  • Exoglycosidases
  • HPLC-Chip/MS technology
  • Human milk oligosaccharides
  • Infrared multiphoton dissociation
  • Matrix-assisted laser desorption/ionization Fourier transform mass spectrometry
  • Milk glycome

ASJC Scopus subject areas

  • Agricultural and Biological Sciences (miscellaneous)
  • Food Science
  • Chemistry (miscellaneous)

Cite this

Ninonuevo, M. R., Park, Y., Yin, H., Zhang, J., Ward, R. E., Clowers, B. H., ... Lebrilla, C. B. (2006). A strategy for annotating the human milk glycome. Journal of Agricultural and Food Chemistry, 54(20), 7471-7480. https://doi.org/10.1021/jf0615810

A strategy for annotating the human milk glycome. / Ninonuevo, Milady R.; Park, Youmie; Yin, Hongfeng; Zhang, Jinhua; Ward, Robert E.; Clowers, Brian H.; German, J. Bruce; Freeman, Samara L.; Killeen, Kevin; Grimm, Rudolf; Lebrilla, Carlito B.

In: Journal of Agricultural and Food Chemistry, Vol. 54, No. 20, 04.10.2006, p. 7471-7480.

Research output: Contribution to journalArticle

Ninonuevo, MR, Park, Y, Yin, H, Zhang, J, Ward, RE, Clowers, BH, German, JB, Freeman, SL, Killeen, K, Grimm, R & Lebrilla, CB 2006, 'A strategy for annotating the human milk glycome', Journal of Agricultural and Food Chemistry, vol. 54, no. 20, pp. 7471-7480. https://doi.org/10.1021/jf0615810
Ninonuevo MR, Park Y, Yin H, Zhang J, Ward RE, Clowers BH et al. A strategy for annotating the human milk glycome. Journal of Agricultural and Food Chemistry. 2006 Oct 4;54(20):7471-7480. https://doi.org/10.1021/jf0615810
Ninonuevo, Milady R. ; Park, Youmie ; Yin, Hongfeng ; Zhang, Jinhua ; Ward, Robert E. ; Clowers, Brian H. ; German, J. Bruce ; Freeman, Samara L. ; Killeen, Kevin ; Grimm, Rudolf ; Lebrilla, Carlito B. / A strategy for annotating the human milk glycome. In: Journal of Agricultural and Food Chemistry. 2006 ; Vol. 54, No. 20. pp. 7471-7480.
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