A strain of Fujinami sarcoma virus which is temperature-sensitive in protein phosphorylation and cellular transformation

Tony Pawson, Jerry Guyden, Tsu Hsun Kung, Kathryn Radke, Thomas Gilmore, G. Steven Martin

Research output: Contribution to journalArticle

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Abstract

Cells infected by one strain of Fujinami sarcoma virus (FSV) are transformed at 38°C but are phenotypically normal at 41.5°C. FSV encodes a 140,000 molecular weight protein (P140) with gag gene-related and FSV-specific peptide sequences. At 41.5°C, P140 is weakly phosphorylated at serine residues, and is inactive in the immune complex protein kinase assay. At 38°C, P140 is highly phosphorylated, contains phosphotyrosine in addition to phosphoserine, and in the immune complex kinase assay becomes phosphorylated at three tyrosine residues. Phosphorylation of cellular polypeptides at tyrosine residues in FSV-infected cells is also temperature-sensitive. These observations indicate that P140 is the transforming protein of FSV and that protein phosphorylation at tyrosine residues is involved in transformation by this virus.

Original languageEnglish (US)
Pages (from-to)767-775
Number of pages9
JournalCell
Volume22
Issue number3
DOIs
StatePublished - 1980

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Avian Sarcoma Viruses
Phosphorylation
Viruses
Temperature
Tyrosine
Antigen-Antibody Complex
Proteins
Assays
gag Genes
Phosphoserine
Peptides
Phosphotyrosine
Protein Kinases
Serine
Phosphotransferases
Molecular Weight
Genes
Molecular weight

ASJC Scopus subject areas

  • Cell Biology
  • Molecular Biology
  • Medicine(all)

Cite this

A strain of Fujinami sarcoma virus which is temperature-sensitive in protein phosphorylation and cellular transformation. / Pawson, Tony; Guyden, Jerry; Kung, Tsu Hsun; Radke, Kathryn; Gilmore, Thomas; Steven Martin, G.

In: Cell, Vol. 22, No. 3, 1980, p. 767-775.

Research output: Contribution to journalArticle

Pawson, T, Guyden, J, Kung, TH, Radke, K, Gilmore, T & Steven Martin, G 1980, 'A strain of Fujinami sarcoma virus which is temperature-sensitive in protein phosphorylation and cellular transformation', Cell, vol. 22, no. 3, pp. 767-775. https://doi.org/10.1016/0092-8674(80)90553-X
Pawson, Tony ; Guyden, Jerry ; Kung, Tsu Hsun ; Radke, Kathryn ; Gilmore, Thomas ; Steven Martin, G. / A strain of Fujinami sarcoma virus which is temperature-sensitive in protein phosphorylation and cellular transformation. In: Cell. 1980 ; Vol. 22, No. 3. pp. 767-775.
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abstract = "Cells infected by one strain of Fujinami sarcoma virus (FSV) are transformed at 38°C but are phenotypically normal at 41.5°C. FSV encodes a 140,000 molecular weight protein (P140) with gag gene-related and FSV-specific peptide sequences. At 41.5°C, P140 is weakly phosphorylated at serine residues, and is inactive in the immune complex protein kinase assay. At 38°C, P140 is highly phosphorylated, contains phosphotyrosine in addition to phosphoserine, and in the immune complex kinase assay becomes phosphorylated at three tyrosine residues. Phosphorylation of cellular polypeptides at tyrosine residues in FSV-infected cells is also temperature-sensitive. These observations indicate that P140 is the transforming protein of FSV and that protein phosphorylation at tyrosine residues is involved in transformation by this virus.",
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AU - Steven Martin, G.

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