A statistical model to optimize enzyme-linked immunosorbent assay parameters for detection of Mycoplasma gallisepticum and M. synoviae antibodies in egg yolk.

H. O. Mohammed, R. Yamamoto, Tim Carpenter, H. B. Ortmayer

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

An enzyme-linked immunosorbent assay (ELISA) was developed to quantify Mycoplasma gallisepticum (MG) and M. synoviae (MS) antibodies in egg-yolk extract. Various parameters of ELISA were evaluated and optimized. A statistical model was developed to study the relationship between ELISA absorbance (A) and antigen concentration, antibody concentration, and time of reading of the test. These factors explained 62% of the variability in A for the MG antigen and 74% of the variability in A for the MS antigen. The optimum concentration of MG antigen was 4 micrograms protein/ml, and that for MS antigen was 3 micrograms protein/ml. A similar model was developed to study the relation between A and conjugate concentration, antibody concentration, and time of reading of the test. The optimum concentration of the conjugate was found to be 1:4000. The within-run and between-run coefficients of variation for MG were 8% and 9%, respectively, and those for MS were 9% and 7%, respectively, indicating a high degree of reproducibility of these tests. There was a high correlation between ELISA and hemagglutination-inhibition test results.

Original languageEnglish (US)
Pages (from-to)389-397
Number of pages9
JournalAvian Diseases
Volume30
Issue number2
DOIs
StatePublished - Jan 1 1986
Externally publishedYes

Fingerprint

Mycoplasma gallisepticum
Mycoplasma synoviae
Egg Yolk
Statistical Models
statistical models
egg yolk
Enzyme-Linked Immunosorbent Assay
enzyme-linked immunosorbent assay
antigens
Antigens
antibodies
Antibodies
Reading
Hemagglutination Inhibition Tests
hemagglutination inhibition test
testing
reproducibility
absorbance
Proteins
proteins

ASJC Scopus subject areas

  • Food Animals
  • Animal Science and Zoology
  • Immunology and Microbiology(all)

Cite this

A statistical model to optimize enzyme-linked immunosorbent assay parameters for detection of Mycoplasma gallisepticum and M. synoviae antibodies in egg yolk. / Mohammed, H. O.; Yamamoto, R.; Carpenter, Tim; Ortmayer, H. B.

In: Avian Diseases, Vol. 30, No. 2, 01.01.1986, p. 389-397.

Research output: Contribution to journalArticle

@article{f0c0275b70d4400d95eb944aca54e807,
title = "A statistical model to optimize enzyme-linked immunosorbent assay parameters for detection of Mycoplasma gallisepticum and M. synoviae antibodies in egg yolk.",
abstract = "An enzyme-linked immunosorbent assay (ELISA) was developed to quantify Mycoplasma gallisepticum (MG) and M. synoviae (MS) antibodies in egg-yolk extract. Various parameters of ELISA were evaluated and optimized. A statistical model was developed to study the relationship between ELISA absorbance (A) and antigen concentration, antibody concentration, and time of reading of the test. These factors explained 62{\%} of the variability in A for the MG antigen and 74{\%} of the variability in A for the MS antigen. The optimum concentration of MG antigen was 4 micrograms protein/ml, and that for MS antigen was 3 micrograms protein/ml. A similar model was developed to study the relation between A and conjugate concentration, antibody concentration, and time of reading of the test. The optimum concentration of the conjugate was found to be 1:4000. The within-run and between-run coefficients of variation for MG were 8{\%} and 9{\%}, respectively, and those for MS were 9{\%} and 7{\%}, respectively, indicating a high degree of reproducibility of these tests. There was a high correlation between ELISA and hemagglutination-inhibition test results.",
author = "Mohammed, {H. O.} and R. Yamamoto and Tim Carpenter and Ortmayer, {H. B.}",
year = "1986",
month = "1",
day = "1",
doi = "10.2307/1590546",
language = "English (US)",
volume = "30",
pages = "389--397",
journal = "Avian Diseases",
issn = "0005-2086",
publisher = "American Association of Avian Pathologists",
number = "2",

}

TY - JOUR

T1 - A statistical model to optimize enzyme-linked immunosorbent assay parameters for detection of Mycoplasma gallisepticum and M. synoviae antibodies in egg yolk.

AU - Mohammed, H. O.

AU - Yamamoto, R.

AU - Carpenter, Tim

AU - Ortmayer, H. B.

PY - 1986/1/1

Y1 - 1986/1/1

N2 - An enzyme-linked immunosorbent assay (ELISA) was developed to quantify Mycoplasma gallisepticum (MG) and M. synoviae (MS) antibodies in egg-yolk extract. Various parameters of ELISA were evaluated and optimized. A statistical model was developed to study the relationship between ELISA absorbance (A) and antigen concentration, antibody concentration, and time of reading of the test. These factors explained 62% of the variability in A for the MG antigen and 74% of the variability in A for the MS antigen. The optimum concentration of MG antigen was 4 micrograms protein/ml, and that for MS antigen was 3 micrograms protein/ml. A similar model was developed to study the relation between A and conjugate concentration, antibody concentration, and time of reading of the test. The optimum concentration of the conjugate was found to be 1:4000. The within-run and between-run coefficients of variation for MG were 8% and 9%, respectively, and those for MS were 9% and 7%, respectively, indicating a high degree of reproducibility of these tests. There was a high correlation between ELISA and hemagglutination-inhibition test results.

AB - An enzyme-linked immunosorbent assay (ELISA) was developed to quantify Mycoplasma gallisepticum (MG) and M. synoviae (MS) antibodies in egg-yolk extract. Various parameters of ELISA were evaluated and optimized. A statistical model was developed to study the relationship between ELISA absorbance (A) and antigen concentration, antibody concentration, and time of reading of the test. These factors explained 62% of the variability in A for the MG antigen and 74% of the variability in A for the MS antigen. The optimum concentration of MG antigen was 4 micrograms protein/ml, and that for MS antigen was 3 micrograms protein/ml. A similar model was developed to study the relation between A and conjugate concentration, antibody concentration, and time of reading of the test. The optimum concentration of the conjugate was found to be 1:4000. The within-run and between-run coefficients of variation for MG were 8% and 9%, respectively, and those for MS were 9% and 7%, respectively, indicating a high degree of reproducibility of these tests. There was a high correlation between ELISA and hemagglutination-inhibition test results.

UR - http://www.scopus.com/inward/record.url?scp=0022694513&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0022694513&partnerID=8YFLogxK

U2 - 10.2307/1590546

DO - 10.2307/1590546

M3 - Article

C2 - 3729885

AN - SCOPUS:0022694513

VL - 30

SP - 389

EP - 397

JO - Avian Diseases

JF - Avian Diseases

SN - 0005-2086

IS - 2

ER -