A single positively selected West Nile viral mutation confers increased virogenesis in American crows

Aaron Brault, Claire Y H Huang, Stanley A. Langevin, Richard M. Kinney, Richard A. Bowen, Wanichaya N. Ramey, Nicholas A. Panella, Edward C. Holmes, Ann M. Powers, Barry R. Miller

Research output: Contribution to journalArticle

224 Citations (Scopus)

Abstract

West Nile virus (WNV), first recognized in North America in 1999, has been responsible for the largest arboviral epiornitic and epidemic of human encephalitis in recorded history. Despite the well-described epidemiological patterns of WNV in North America, the basis for the emergence of WNV-associated avian pathology, particularly in the American crow (AMCR) sentinel species, and the large scale of the North American epidemic and epiornitic is uncertain. We report here that the introduction of a T249P amino acid substitution in the NS3 helicase (found in North American WNV) in a low-virulence strain was sufficient to generate a phenotype highly virulent to AMCRs. Furthermore, comparative sequence analyses of full-length WNV genomes demonstrated that the same site (NS3-249) was subject to adaptive evolution. These phenotypic and evolutionary results provide compelling evidence for the positive selection of a mutation encoding increased viremia potential and virulence in the AMCR sentinel bird species.

Original languageEnglish (US)
Pages (from-to)1162-1166
Number of pages5
JournalNature Genetics
Volume39
Issue number9
DOIs
StatePublished - Sep 1 2007

Fingerprint

Crows
West Nile virus
Mutation
North America
Virulence
Arbovirus Encephalitis
Viremia
Amino Acid Substitution
Birds
Sequence Analysis
History
Genome
Pathology
Phenotype

ASJC Scopus subject areas

  • Genetics(clinical)
  • Genetics

Cite this

Brault, A., Huang, C. Y. H., Langevin, S. A., Kinney, R. M., Bowen, R. A., Ramey, W. N., ... Miller, B. R. (2007). A single positively selected West Nile viral mutation confers increased virogenesis in American crows. Nature Genetics, 39(9), 1162-1166. https://doi.org/10.1038/ng2097

A single positively selected West Nile viral mutation confers increased virogenesis in American crows. / Brault, Aaron; Huang, Claire Y H; Langevin, Stanley A.; Kinney, Richard M.; Bowen, Richard A.; Ramey, Wanichaya N.; Panella, Nicholas A.; Holmes, Edward C.; Powers, Ann M.; Miller, Barry R.

In: Nature Genetics, Vol. 39, No. 9, 01.09.2007, p. 1162-1166.

Research output: Contribution to journalArticle

Brault, A, Huang, CYH, Langevin, SA, Kinney, RM, Bowen, RA, Ramey, WN, Panella, NA, Holmes, EC, Powers, AM & Miller, BR 2007, 'A single positively selected West Nile viral mutation confers increased virogenesis in American crows', Nature Genetics, vol. 39, no. 9, pp. 1162-1166. https://doi.org/10.1038/ng2097
Brault, Aaron ; Huang, Claire Y H ; Langevin, Stanley A. ; Kinney, Richard M. ; Bowen, Richard A. ; Ramey, Wanichaya N. ; Panella, Nicholas A. ; Holmes, Edward C. ; Powers, Ann M. ; Miller, Barry R. / A single positively selected West Nile viral mutation confers increased virogenesis in American crows. In: Nature Genetics. 2007 ; Vol. 39, No. 9. pp. 1162-1166.
@article{803197c55c27491bb0dc770354c99d1a,
title = "A single positively selected West Nile viral mutation confers increased virogenesis in American crows",
abstract = "West Nile virus (WNV), first recognized in North America in 1999, has been responsible for the largest arboviral epiornitic and epidemic of human encephalitis in recorded history. Despite the well-described epidemiological patterns of WNV in North America, the basis for the emergence of WNV-associated avian pathology, particularly in the American crow (AMCR) sentinel species, and the large scale of the North American epidemic and epiornitic is uncertain. We report here that the introduction of a T249P amino acid substitution in the NS3 helicase (found in North American WNV) in a low-virulence strain was sufficient to generate a phenotype highly virulent to AMCRs. Furthermore, comparative sequence analyses of full-length WNV genomes demonstrated that the same site (NS3-249) was subject to adaptive evolution. These phenotypic and evolutionary results provide compelling evidence for the positive selection of a mutation encoding increased viremia potential and virulence in the AMCR sentinel bird species.",
author = "Aaron Brault and Huang, {Claire Y H} and Langevin, {Stanley A.} and Kinney, {Richard M.} and Bowen, {Richard A.} and Ramey, {Wanichaya N.} and Panella, {Nicholas A.} and Holmes, {Edward C.} and Powers, {Ann M.} and Miller, {Barry R.}",
year = "2007",
month = "9",
day = "1",
doi = "10.1038/ng2097",
language = "English (US)",
volume = "39",
pages = "1162--1166",
journal = "Nature Genetics",
issn = "1061-4036",
publisher = "Nature Publishing Group",
number = "9",

}

TY - JOUR

T1 - A single positively selected West Nile viral mutation confers increased virogenesis in American crows

AU - Brault, Aaron

AU - Huang, Claire Y H

AU - Langevin, Stanley A.

AU - Kinney, Richard M.

AU - Bowen, Richard A.

AU - Ramey, Wanichaya N.

AU - Panella, Nicholas A.

AU - Holmes, Edward C.

AU - Powers, Ann M.

AU - Miller, Barry R.

PY - 2007/9/1

Y1 - 2007/9/1

N2 - West Nile virus (WNV), first recognized in North America in 1999, has been responsible for the largest arboviral epiornitic and epidemic of human encephalitis in recorded history. Despite the well-described epidemiological patterns of WNV in North America, the basis for the emergence of WNV-associated avian pathology, particularly in the American crow (AMCR) sentinel species, and the large scale of the North American epidemic and epiornitic is uncertain. We report here that the introduction of a T249P amino acid substitution in the NS3 helicase (found in North American WNV) in a low-virulence strain was sufficient to generate a phenotype highly virulent to AMCRs. Furthermore, comparative sequence analyses of full-length WNV genomes demonstrated that the same site (NS3-249) was subject to adaptive evolution. These phenotypic and evolutionary results provide compelling evidence for the positive selection of a mutation encoding increased viremia potential and virulence in the AMCR sentinel bird species.

AB - West Nile virus (WNV), first recognized in North America in 1999, has been responsible for the largest arboviral epiornitic and epidemic of human encephalitis in recorded history. Despite the well-described epidemiological patterns of WNV in North America, the basis for the emergence of WNV-associated avian pathology, particularly in the American crow (AMCR) sentinel species, and the large scale of the North American epidemic and epiornitic is uncertain. We report here that the introduction of a T249P amino acid substitution in the NS3 helicase (found in North American WNV) in a low-virulence strain was sufficient to generate a phenotype highly virulent to AMCRs. Furthermore, comparative sequence analyses of full-length WNV genomes demonstrated that the same site (NS3-249) was subject to adaptive evolution. These phenotypic and evolutionary results provide compelling evidence for the positive selection of a mutation encoding increased viremia potential and virulence in the AMCR sentinel bird species.

UR - http://www.scopus.com/inward/record.url?scp=34548333546&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=34548333546&partnerID=8YFLogxK

U2 - 10.1038/ng2097

DO - 10.1038/ng2097

M3 - Article

C2 - 17694056

AN - SCOPUS:34548333546

VL - 39

SP - 1162

EP - 1166

JO - Nature Genetics

JF - Nature Genetics

SN - 1061-4036

IS - 9

ER -