A single-electron reducing quinone oxidoreductase is necessary to induce haustorium development in the root parasitic plant Triphysaria

Pradeepa C.G. Bandaranayake, Tatiana Filappova, Alexey Tomilov, Natalya B. Tomilova, Denneal Jamison-Mcclung, Quy Ngo, Kentaro Inoue, John I. Yoder

Research output: Contribution to journalArticle

42 Citations (Scopus)

Abstract

Parasitic plants in the Orobanchaceae develop haustoria in response to contact with host roots or chemical haustoriainducing factors. Experiments in this manuscript test the hypothesis that quinolic-inducing factors activate haustorium development via a signal mechanism initiated by redox cycling between quinone and hydroquinone states. Two cDNAs were previously isolated from roots of the parasitic plant Triphysaria versicolor that encode distinct quinone oxidoreductases. QR1 encodes a single-electron reducing NADPH quinone oxidoreductase similar to z-crystallin. The QR2 enzyme catalyzes two electron reductions typical of xenobiotic detoxification. QR1 and QR2 transcripts are upregulated in a primary response to chemical-inducing factors, but only QR1 was upregulated in response to host roots. RNA interference technology was used to reduce QR1 and QR2 transcripts in Triphysaria roots that were evaluated for their ability to form haustoria. There was a significant decrease in haustorium development in roots silenced for QR1 but not in roots silenced for QR2. The infrequent QR1 transgenic roots that did develop haustoria had levels of QR1 similar to those of nontransgenic roots. These experiments implicate QR1 as one of the earliest genes on the haustorium signal transduction pathway, encoding a quinone oxidoreductase necessary for the redox bioactivation of haustorial inducing factors.

Original languageEnglish (US)
Pages (from-to)1404-1419
Number of pages16
JournalPlant Cell
Volume22
Issue number4
DOIs
StatePublished - Apr 1 2010

Fingerprint

Plant Roots
parasitic plants
oxidoreductases
quinones
Oxidoreductases
electrons
Electrons
Oxidation-Reduction
Orobanchaceae
crystallins
hydroquinone
Crystallins
Xenobiotics
xenobiotics
RNA Interference
RNA interference
NADP
NADP (coenzyme)
signal transduction
Signal Transduction

ASJC Scopus subject areas

  • Plant Science
  • Cell Biology

Cite this

Bandaranayake, P. C. G., Filappova, T., Tomilov, A., Tomilova, N. B., Jamison-Mcclung, D., Ngo, Q., ... Yoder, J. I. (2010). A single-electron reducing quinone oxidoreductase is necessary to induce haustorium development in the root parasitic plant Triphysaria. Plant Cell, 22(4), 1404-1419. https://doi.org/10.1105/tpc.110.074831

A single-electron reducing quinone oxidoreductase is necessary to induce haustorium development in the root parasitic plant Triphysaria. / Bandaranayake, Pradeepa C.G.; Filappova, Tatiana; Tomilov, Alexey; Tomilova, Natalya B.; Jamison-Mcclung, Denneal; Ngo, Quy; Inoue, Kentaro; Yoder, John I.

In: Plant Cell, Vol. 22, No. 4, 01.04.2010, p. 1404-1419.

Research output: Contribution to journalArticle

Bandaranayake, PCG, Filappova, T, Tomilov, A, Tomilova, NB, Jamison-Mcclung, D, Ngo, Q, Inoue, K & Yoder, JI 2010, 'A single-electron reducing quinone oxidoreductase is necessary to induce haustorium development in the root parasitic plant Triphysaria', Plant Cell, vol. 22, no. 4, pp. 1404-1419. https://doi.org/10.1105/tpc.110.074831
Bandaranayake, Pradeepa C.G. ; Filappova, Tatiana ; Tomilov, Alexey ; Tomilova, Natalya B. ; Jamison-Mcclung, Denneal ; Ngo, Quy ; Inoue, Kentaro ; Yoder, John I. / A single-electron reducing quinone oxidoreductase is necessary to induce haustorium development in the root parasitic plant Triphysaria. In: Plant Cell. 2010 ; Vol. 22, No. 4. pp. 1404-1419.
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