A single charged surface residue modifies the activity of ikitoxin, a beta-type Na+ channel toxin from Parabuthus transvaalicus

A. Bora Inceoglu, Yuki Hayashida, Jozsef Lango, Andrew Ishida, Bruce D. Hammock

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

We previously purified and characterized a peptide toxin, birtoxin, from the South African scorpion Parabuthus transvaalicus. Birtoxin is a 58-residue, long chain neurotoxin that has a unique three disulfide-bridged structure. Here we report the isolation and characterization of ikitoxin, a peptide toxin with a single residue difference, and a markedly reduced biological activity, from birtoxin. Bioassays on mice showed that high doses of ikitoxin induce unprovoked jumps, whereas birtoxin induces jumps at a 1000-fold lower concentration. Both toxins are active against mice when administered intracerebroventricularly. Mass determination indicated an apparent mass of 6615 Da for ikitoxin vs. 6543 Da for birtoxin. Amino acid sequence determination revealed that the amino-acid sequence of ikitoxin differs from birtoxin by a single residue change from glycine to glutamic acid at position 23, consistent with the apparent mass difference of 72 Da. This single-residue difference renders ikitoxin much less effective in producing the same behavioral effect as low concentrations of birtoxin. Electrophysiological measurements showed that birtoxin and ikitoxin can be classified as beta group toxins for voltage-gated Na+ channels of central neurons. It is our conclusion that the N-terminal loop preceding the α-helix in scorpion toxins is one of the determinative domains in the interaction of toxins with the target ion channel.

Original languageEnglish (US)
Pages (from-to)5369-5376
Number of pages8
JournalEuropean Journal of Biochemistry
Volume269
Issue number22
DOIs
StatePublished - 2002

Fingerprint

Scorpions
Amino Acids
Peptides
birtoxin
Bioassay
Protein Sequence Analysis
Neurotoxins
Bioactivity
Ion Channels
Disulfides
Biological Assay
Glycine
Neurons
Glutamic Acid
Amino Acid Sequence
Electric potential

Keywords

  • Birtoxin
  • Ikitoxin
  • Parabuthus
  • Scorpion
  • Voltage-gated Na current

ASJC Scopus subject areas

  • Biochemistry

Cite this

A single charged surface residue modifies the activity of ikitoxin, a beta-type Na+ channel toxin from Parabuthus transvaalicus. / Inceoglu, A. Bora; Hayashida, Yuki; Lango, Jozsef; Ishida, Andrew; Hammock, Bruce D.

In: European Journal of Biochemistry, Vol. 269, No. 22, 2002, p. 5369-5376.

Research output: Contribution to journalArticle

Inceoglu, A. Bora ; Hayashida, Yuki ; Lango, Jozsef ; Ishida, Andrew ; Hammock, Bruce D. / A single charged surface residue modifies the activity of ikitoxin, a beta-type Na+ channel toxin from Parabuthus transvaalicus. In: European Journal of Biochemistry. 2002 ; Vol. 269, No. 22. pp. 5369-5376.
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AB - We previously purified and characterized a peptide toxin, birtoxin, from the South African scorpion Parabuthus transvaalicus. Birtoxin is a 58-residue, long chain neurotoxin that has a unique three disulfide-bridged structure. Here we report the isolation and characterization of ikitoxin, a peptide toxin with a single residue difference, and a markedly reduced biological activity, from birtoxin. Bioassays on mice showed that high doses of ikitoxin induce unprovoked jumps, whereas birtoxin induces jumps at a 1000-fold lower concentration. Both toxins are active against mice when administered intracerebroventricularly. Mass determination indicated an apparent mass of 6615 Da for ikitoxin vs. 6543 Da for birtoxin. Amino acid sequence determination revealed that the amino-acid sequence of ikitoxin differs from birtoxin by a single residue change from glycine to glutamic acid at position 23, consistent with the apparent mass difference of 72 Da. This single-residue difference renders ikitoxin much less effective in producing the same behavioral effect as low concentrations of birtoxin. Electrophysiological measurements showed that birtoxin and ikitoxin can be classified as beta group toxins for voltage-gated Na+ channels of central neurons. It is our conclusion that the N-terminal loop preceding the α-helix in scorpion toxins is one of the determinative domains in the interaction of toxins with the target ion channel.

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