A single-center, randomized double-blind placebo-controlled study evaluating the effects of poly-gamma-glutamate on human NK cell activity after an 8-week oral administration in healthy volunteers

Kyung Soo Kim, Tae Young Lee, Jang Hee Hong, Ahrom Kim, Sung Jin Kim, Jai Chul Choi, Moon Hee Sung, Haryoung Poo

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Abstract

A randomized double-blind placebo-controlled immunity study involving 99 healthy volunteers was performed to investigate the effect of poly-γ-glutamate (γ-PGA) on human natural killer (NK) cell activity in peripheral blood. The volunteers were randomly assigned to one of three groups and orally treated with solutions (25 mL) containing 0 mg (placebo), 250 mg (low dosage), or 500 mg (high dosage) of γ-PGA. Each volunteer took one dose every 12 hours for 8 weeks. Blood samples were drawn before the initial treatment and at the 4th and the 8th weeks of treatment. NK cell activity was assessed by measuring its degranulation, cytokine production, and cytotoxicity against the K562 cell line. Our results revealed that the cytotoxic activities of NK cells from the high-dosage γ-PGA group were significantly higher (P<0.05 for all comparisons) compared to the low dosage and placebo groups at weeks 4 and 8 after the initial treatment. This increase in the NK cell activity among peripheral blood mononuclear cells (PBMCs) of healthy individuals was also confirmed in vitro (as assessed by the degranulation and cytokine production). These results suggest that the oral administration of γ-PGA induces a cell-mediated immunity by increasing the NK cell activity in humans.

Original languageEnglish (US)
Article number635960
JournalEvidence-based Complementary and Alternative Medicine
Volume2013
DOIs
StatePublished - 2013
Externally publishedYes

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Natural Killer Cells
Oral Administration
Healthy Volunteers
Prostaglandins A
Placebos
Volunteers
Cytokines
K562 Cells
Human Activities
Cellular Immunity
Glutamic Acid
Immunity
Blood Cells
Therapeutics
poly(gamma-glutamic acid)
Cell Line

ASJC Scopus subject areas

  • Complementary and alternative medicine

Cite this

A single-center, randomized double-blind placebo-controlled study evaluating the effects of poly-gamma-glutamate on human NK cell activity after an 8-week oral administration in healthy volunteers. / Kim, Kyung Soo; Lee, Tae Young; Hong, Jang Hee; Kim, Ahrom; Kim, Sung Jin; Choi, Jai Chul; Sung, Moon Hee; Poo, Haryoung.

In: Evidence-based Complementary and Alternative Medicine, Vol. 2013, 635960, 2013.

Research output: Contribution to journalArticle

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abstract = "A randomized double-blind placebo-controlled immunity study involving 99 healthy volunteers was performed to investigate the effect of poly-γ-glutamate (γ-PGA) on human natural killer (NK) cell activity in peripheral blood. The volunteers were randomly assigned to one of three groups and orally treated with solutions (25 mL) containing 0 mg (placebo), 250 mg (low dosage), or 500 mg (high dosage) of γ-PGA. Each volunteer took one dose every 12 hours for 8 weeks. Blood samples were drawn before the initial treatment and at the 4th and the 8th weeks of treatment. NK cell activity was assessed by measuring its degranulation, cytokine production, and cytotoxicity against the K562 cell line. Our results revealed that the cytotoxic activities of NK cells from the high-dosage γ-PGA group were significantly higher (P<0.05 for all comparisons) compared to the low dosage and placebo groups at weeks 4 and 8 after the initial treatment. This increase in the NK cell activity among peripheral blood mononuclear cells (PBMCs) of healthy individuals was also confirmed in vitro (as assessed by the degranulation and cytokine production). These results suggest that the oral administration of γ-PGA induces a cell-mediated immunity by increasing the NK cell activity in humans.",
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