A single-cell model to study changes in neuronal fractal dimension

Laura N Borodinsky; Mónica L. Fiszman

doi:10.1006/meth.2001.1204

A single-cell model to study changes in neuronal fractal dimension

Laura N Borodinsky, Mónica L. Fiszman

Research output: Contribution to journal › Article › peer-review

13 Scopus citations

Abstract

Many methods have been developed to quantify neuronal morphology: measurement of neurite length, neurite number, etc. However, none of these approaches provides a comprehensive view of the complexity of neuronal morphology. In this work we have analyzed the evaluation of fractal dimension (D) as a tool to represent and quantify changes in complexity of the dendritic arbor, in in vitro cultures grown under Iow-density conditions. Neurons grown in isolation developed a bipolar morphology corresponding to a fractal dimension close to the unit. The analysis showed that neuronal complexity increased when cells were incubated with a depolarizing potassium concentration and there was a correlation with an increase in fractal dimension (D₅ mM KCI = 1.08 ± 0.01, D₂₅ mM KCI = 1.25 ± 0.01). We conclude that fractal dimension is a suitable parameter to quantify changes in neuronal morphological complexity.

Original language	English (US)
Pages (from-to)	341-345
Number of pages	5
Journal	Methods
Volume	24
Issue number	4
DOIs	https://doi.org/10.1006/meth.2001.1204
State	Published - 2001
Externally published	Yes

Keywords

Cerebellar granule cells
Fractal dimension
Neural activity
Neuronal complexity

ASJC Scopus subject areas

Molecular Biology

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10.1006/meth.2001.1204

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abstract = "Many methods have been developed to quantify neuronal morphology: measurement of neurite length, neurite number, etc. However, none of these approaches provides a comprehensive view of the complexity of neuronal morphology. In this work we have analyzed the evaluation of fractal dimension (D) as a tool to represent and quantify changes in complexity of the dendritic arbor, in in vitro cultures grown under Iow-density conditions. Neurons grown in isolation developed a bipolar morphology corresponding to a fractal dimension close to the unit. The analysis showed that neuronal complexity increased when cells were incubated with a depolarizing potassium concentration and there was a correlation with an increase in fractal dimension (D5 mM KCI = 1.08 ± 0.01, D25 mM KCI = 1.25 ± 0.01). We conclude that fractal dimension is a suitable parameter to quantify changes in neuronal morphological complexity.",

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