A single-cell model to study changes in neuronal fractal dimension

Many methods have been developed to quantify neuronal morphology: measurement of neurite length, neurite number, etc. However, none of these approaches provides a comprehensive view of the complexity of neuronal morphology. In this work we have analyzed the evaluation of fractal dimension (D) as a tool to represent and quantify changes in complexity of the dendritic arbor, in in vitro cultures grown under Iow-density conditions. Neurons grown in isolation developed a bipolar morphology corresponding to a fractal dimension close to the unit. The analysis showed that neuronal complexity increased when cells were incubated with a depolarizing potassium concentration and there was a correlation with an increase in fractal dimension (D₅ mM KCI = 1.08 ± 0.01, D₂₅ mM KCI = 1.25 ± 0.01). We conclude that fractal dimension is a suitable parameter to quantify changes in neuronal morphological complexity.