Abstract
Many methods have been developed to quantify neuronal morphology: measurement of neurite length, neurite number, etc. However, none of these approaches provides a comprehensive view of the complexity of neuronal morphology. In this work we have analyzed the evaluation of fractal dimension (D) as a tool to represent and quantify changes in complexity of the dendritic arbor, in in vitro cultures grown under Iow-density conditions. Neurons grown in isolation developed a bipolar morphology corresponding to a fractal dimension close to the unit. The analysis showed that neuronal complexity increased when cells were incubated with a depolarizing potassium concentration and there was a correlation with an increase in fractal dimension (D5 mM KCI = 1.08 ± 0.01, D25 mM KCI = 1.25 ± 0.01). We conclude that fractal dimension is a suitable parameter to quantify changes in neuronal morphological complexity.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 341-345 |
| Number of pages | 5 |
| Journal | Methods |
| Volume | 24 |
| Issue number | 4 |
| DOIs | |
| State | Published - 2001 |
| Externally published | Yes |
Fingerprint
Keywords
- Cerebellar granule cells
- Fractal dimension
- Neural activity
- Neuronal complexity
ASJC Scopus subject areas
- Molecular Biology
Cite this
A single-cell model to study changes in neuronal fractal dimension. / Borodinsky, Laura N; Fiszman, Mónica L.
In: Methods, Vol. 24, No. 4, 2001, p. 341-345.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - A single-cell model to study changes in neuronal fractal dimension
AU - Borodinsky, Laura N
AU - Fiszman, Mónica L.
PY - 2001
Y1 - 2001
N2 - Many methods have been developed to quantify neuronal morphology: measurement of neurite length, neurite number, etc. However, none of these approaches provides a comprehensive view of the complexity of neuronal morphology. In this work we have analyzed the evaluation of fractal dimension (D) as a tool to represent and quantify changes in complexity of the dendritic arbor, in in vitro cultures grown under Iow-density conditions. Neurons grown in isolation developed a bipolar morphology corresponding to a fractal dimension close to the unit. The analysis showed that neuronal complexity increased when cells were incubated with a depolarizing potassium concentration and there was a correlation with an increase in fractal dimension (D5 mM KCI = 1.08 ± 0.01, D25 mM KCI = 1.25 ± 0.01). We conclude that fractal dimension is a suitable parameter to quantify changes in neuronal morphological complexity.
AB - Many methods have been developed to quantify neuronal morphology: measurement of neurite length, neurite number, etc. However, none of these approaches provides a comprehensive view of the complexity of neuronal morphology. In this work we have analyzed the evaluation of fractal dimension (D) as a tool to represent and quantify changes in complexity of the dendritic arbor, in in vitro cultures grown under Iow-density conditions. Neurons grown in isolation developed a bipolar morphology corresponding to a fractal dimension close to the unit. The analysis showed that neuronal complexity increased when cells were incubated with a depolarizing potassium concentration and there was a correlation with an increase in fractal dimension (D5 mM KCI = 1.08 ± 0.01, D25 mM KCI = 1.25 ± 0.01). We conclude that fractal dimension is a suitable parameter to quantify changes in neuronal morphological complexity.
KW - Cerebellar granule cells
KW - Fractal dimension
KW - Neural activity
KW - Neuronal complexity
UR - http://www.scopus.com/inward/record.url?scp=0034840619&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0034840619&partnerID=8YFLogxK
U2 - 10.1006/meth.2001.1204
DO - 10.1006/meth.2001.1204
M3 - Article
C2 - 11465999
AN - SCOPUS:0034840619
VL - 24
SP - 341
EP - 345
JO - Methods
JF - Methods
SN - 0076-6879
IS - 4
ER -