A single-cell model to study changes in neuronal fractal dimension

Laura N Borodinsky, Mónica L. Fiszman

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Many methods have been developed to quantify neuronal morphology: measurement of neurite length, neurite number, etc. However, none of these approaches provides a comprehensive view of the complexity of neuronal morphology. In this work we have analyzed the evaluation of fractal dimension (D) as a tool to represent and quantify changes in complexity of the dendritic arbor, in in vitro cultures grown under Iow-density conditions. Neurons grown in isolation developed a bipolar morphology corresponding to a fractal dimension close to the unit. The analysis showed that neuronal complexity increased when cells were incubated with a depolarizing potassium concentration and there was a correlation with an increase in fractal dimension (D5 mM KCI = 1.08 ± 0.01, D25 mM KCI = 1.25 ± 0.01). We conclude that fractal dimension is a suitable parameter to quantify changes in neuronal morphological complexity.

Original languageEnglish (US)
Pages (from-to)341-345
Number of pages5
JournalMethods
Volume24
Issue number4
DOIs
StatePublished - 2001
Externally publishedYes

Fingerprint

Fractals
Fractal dimension
Neurites
Neurons
Potassium

Keywords

  • Cerebellar granule cells
  • Fractal dimension
  • Neural activity
  • Neuronal complexity

ASJC Scopus subject areas

  • Molecular Biology

Cite this

A single-cell model to study changes in neuronal fractal dimension. / Borodinsky, Laura N; Fiszman, Mónica L.

In: Methods, Vol. 24, No. 4, 2001, p. 341-345.

Research output: Contribution to journalArticle

Borodinsky, Laura N ; Fiszman, Mónica L. / A single-cell model to study changes in neuronal fractal dimension. In: Methods. 2001 ; Vol. 24, No. 4. pp. 341-345.
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