A simple method for site-directed mutagenesis using the polymerase chain reaction

A. Hemsley; N. Arnheim; M. D. Toney; Gino A Cortopassi; D. J. Galas

doi:10.1093/nar/17.21.8915

A simple method for site-directed mutagenesis using the polymerase chain reaction

A. Hemsley, N. Arnheim, M. D. Toney, Gino A Cortopassi, D. J. Galas

Research output: Contribution to journal › Article › peer-review

98 Scopus citations

Abstract

Incorrect details regarding PCR conditions were supplied for the Materials and Methods section of this paper. The correct details are published below.PCR conditions. Template DNA (10 fmol) and primer sets (1 μM each) were incubated in a Perkin Elmer Cetus Thermal Cycler in 100 μl reaction volumes containing 10 mM Tris-HCl pH 8.3, 50 mM KCl, 2.5 mM MgCl₂, 10 μg gelatin, 0.2 mM of each of NTP and 2 units Taq polymerase (5).

Original language	English (US)
Pages (from-to)	8915
Number of pages	1
Journal	Nucleic Acids Research
Volume	17
Issue number	21
DOIs	https://doi.org/10.1093/nar/17.21.8915
State	Published - Nov 11 1989
Externally published	Yes

ASJC Scopus subject areas

Statistics, Probability and Uncertainty
Applied Mathematics
Health, Toxicology and Mutagenesis
Toxicology
Genetics(clinical)
Genetics

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AU - Toney, M. D.

AU - Cortopassi, Gino A

AU - Galas, D. J.

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AB - Incorrect details regarding PCR conditions were supplied for the Materials and Methods section of this paper. The correct details are published below.PCR conditions. Template DNA (10 fmol) and primer sets (1 μM each) were incubated in a Perkin Elmer Cetus Thermal Cycler in 100 μl reaction volumes containing 10 mM Tris-HCl pH 8.3, 50 mM KCl, 2.5 mM MgCl2, 10 μg gelatin, 0.2 mM of each of NTP and 2 units Taq polymerase (5).

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A simple method for site-directed mutagenesis using the polymerase chain reaction

Abstract

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