A simple method for site-directed mutagenesis using the polymerase chain reaction

A. Hemsley, N. Arnheim, M. D. Toney, Gino A Cortopassi, D. J. Galas

Research output: Contribution to journalArticle

98 Scopus citations

Abstract

Incorrect details regarding PCR conditions were supplied for the Materials and Methods section of this paper. The correct details are published below.PCR conditions. Template DNA (10 fmol) and primer sets (1 μM each) were incubated in a Perkin Elmer Cetus Thermal Cycler in 100 μl reaction volumes containing 10 mM Tris-HCl pH 8.3, 50 mM KCl, 2.5 mM MgCl2, 10 μg gelatin, 0.2 mM of each of NTP and 2 units Taq polymerase (5).

Original languageEnglish (US)
Pages (from-to)8915
Number of pages1
JournalNucleic Acids Research
Volume17
Issue number21
DOIs
StatePublished - Nov 11 1989
Externally publishedYes

ASJC Scopus subject areas

  • Statistics, Probability and Uncertainty
  • Applied Mathematics
  • Health, Toxicology and Mutagenesis
  • Toxicology
  • Genetics(clinical)
  • Genetics

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