Abstract
Incorrect details regarding PCR conditions were supplied for the Materials and Methods section of this paper. The correct details are published below.PCR conditions. Template DNA (10 fmol) and primer sets (1 μM each) were incubated in a Perkin Elmer Cetus Thermal Cycler in 100 μl reaction volumes containing 10 mM Tris-HCl pH 8.3, 50 mM KCl, 2.5 mM MgCl2, 10 μg gelatin, 0.2 mM of each of NTP and 2 units Taq polymerase (5).
| Original language | English (US) |
|---|---|
| Pages (from-to) | 8915 |
| Number of pages | 1 |
| Journal | Nucleic Acids Research |
| Volume | 17 |
| Issue number | 21 |
| DOIs | |
| State | Published - Nov 11 1989 |
| Externally published | Yes |
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ASJC Scopus subject areas
- Statistics, Probability and Uncertainty
- Applied Mathematics
- Health, Toxicology and Mutagenesis
- Toxicology
- Genetics(clinical)
- Genetics
Cite this
A simple method for site-directed mutagenesis using the polymerase chain reaction. / Hemsley, A.; Arnheim, N.; Toney, M. D.; Cortopassi, Gino A; Galas, D. J.
In: Nucleic Acids Research, Vol. 17, No. 21, 11.11.1989, p. 8915.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - A simple method for site-directed mutagenesis using the polymerase chain reaction
AU - Hemsley, A.
AU - Arnheim, N.
AU - Toney, M. D.
AU - Cortopassi, Gino A
AU - Galas, D. J.
PY - 1989/11/11
Y1 - 1989/11/11
N2 - Incorrect details regarding PCR conditions were supplied for the Materials and Methods section of this paper. The correct details are published below.PCR conditions. Template DNA (10 fmol) and primer sets (1 μM each) were incubated in a Perkin Elmer Cetus Thermal Cycler in 100 μl reaction volumes containing 10 mM Tris-HCl pH 8.3, 50 mM KCl, 2.5 mM MgCl2, 10 μg gelatin, 0.2 mM of each of NTP and 2 units Taq polymerase (5).
AB - Incorrect details regarding PCR conditions were supplied for the Materials and Methods section of this paper. The correct details are published below.PCR conditions. Template DNA (10 fmol) and primer sets (1 μM each) were incubated in a Perkin Elmer Cetus Thermal Cycler in 100 μl reaction volumes containing 10 mM Tris-HCl pH 8.3, 50 mM KCl, 2.5 mM MgCl2, 10 μg gelatin, 0.2 mM of each of NTP and 2 units Taq polymerase (5).
UR - http://www.scopus.com/inward/record.url?scp=77957219390&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=77957219390&partnerID=8YFLogxK
U2 - 10.1093/nar/17.21.8915
DO - 10.1093/nar/17.21.8915
M3 - Article
AN - SCOPUS:77957219390
VL - 17
SP - 8915
JO - Nucleic Acids Research
JF - Nucleic Acids Research
SN - 0305-1048
IS - 21
ER -