Abstract
Microsatellite markers were isolated and developed from Culex pipiens quinquefasciatus Say (Diptera: Culicidae) sampled in Johannesburg, South Africa, to identify those that are broadly useful for analyzing Cx. pipiens complex populations between continents. Suitable loci should be 1) inherited in a codominant Mendelian manner, 2) polymorphic, 3) selectively neutral, 4) randomly associated, 5) without null alleles, and 6) applicable across broad regions and between diverse biotypes. Loci in Cx. p. quinquefasciatus from Johannesburg ranged from two to 17 alleles per locus and expected heterozygosities (H e) were 0.02-0.87. Loci in Cx. p. pipiens L. from Johannesburg had five to 19 alleles per locus and He values ranging from 0.57 to 0.93, whereas those from George, South Africa, had five to 17 alleles per locus and He values ranging from 0.54 to 0.88. Loci in North American mosquitoes were more variable. Cx. p. quinquefasciatus from South Carolina had five to 19 alleles per locus and He values ranging from 0.64 to 0.90, whereas Cx. p. pipiens from Massachusetts had six to 28 alleles per locus and with He values ranging from 0.65 to 0.94. All loci were associated randomly. Overall, four of nine of these new loci satisfied all six criteria for broad utility for analyzing the genetic structure of Cx. pipiens populations.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 145-149 |
| Number of pages | 5 |
| Journal | Journal of Medical Entomology |
| Volume | 44 |
| Issue number | 1 |
| DOIs | |
| State | Published - Jan 2007 |
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Keywords
- Culex p. quinquefasciatus
- Culex pipiens complex
- Microsatellite loci
ASJC Scopus subject areas
- Insect Science
- veterinary(all)
Cite this
A set of broadly applicable microsatellite markers for analyzing the structure of Culex pipiens (Diptera : Culicidae) populations. / Edillo, F. E.; Tripet, F.; McAbee, R. D.; Foppa, I. M.; Lanzaro, Gregory C; Cornel, A. J.; Spielman, A.
In: Journal of Medical Entomology, Vol. 44, No. 1, 01.2007, p. 145-149.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - A set of broadly applicable microsatellite markers for analyzing the structure of Culex pipiens (Diptera
T2 - Culicidae) populations
AU - Edillo, F. E.
AU - Tripet, F.
AU - McAbee, R. D.
AU - Foppa, I. M.
AU - Lanzaro, Gregory C
AU - Cornel, A. J.
AU - Spielman, A.
PY - 2007/1
Y1 - 2007/1
N2 - Microsatellite markers were isolated and developed from Culex pipiens quinquefasciatus Say (Diptera: Culicidae) sampled in Johannesburg, South Africa, to identify those that are broadly useful for analyzing Cx. pipiens complex populations between continents. Suitable loci should be 1) inherited in a codominant Mendelian manner, 2) polymorphic, 3) selectively neutral, 4) randomly associated, 5) without null alleles, and 6) applicable across broad regions and between diverse biotypes. Loci in Cx. p. quinquefasciatus from Johannesburg ranged from two to 17 alleles per locus and expected heterozygosities (H e) were 0.02-0.87. Loci in Cx. p. pipiens L. from Johannesburg had five to 19 alleles per locus and He values ranging from 0.57 to 0.93, whereas those from George, South Africa, had five to 17 alleles per locus and He values ranging from 0.54 to 0.88. Loci in North American mosquitoes were more variable. Cx. p. quinquefasciatus from South Carolina had five to 19 alleles per locus and He values ranging from 0.64 to 0.90, whereas Cx. p. pipiens from Massachusetts had six to 28 alleles per locus and with He values ranging from 0.65 to 0.94. All loci were associated randomly. Overall, four of nine of these new loci satisfied all six criteria for broad utility for analyzing the genetic structure of Cx. pipiens populations.
AB - Microsatellite markers were isolated and developed from Culex pipiens quinquefasciatus Say (Diptera: Culicidae) sampled in Johannesburg, South Africa, to identify those that are broadly useful for analyzing Cx. pipiens complex populations between continents. Suitable loci should be 1) inherited in a codominant Mendelian manner, 2) polymorphic, 3) selectively neutral, 4) randomly associated, 5) without null alleles, and 6) applicable across broad regions and between diverse biotypes. Loci in Cx. p. quinquefasciatus from Johannesburg ranged from two to 17 alleles per locus and expected heterozygosities (H e) were 0.02-0.87. Loci in Cx. p. pipiens L. from Johannesburg had five to 19 alleles per locus and He values ranging from 0.57 to 0.93, whereas those from George, South Africa, had five to 17 alleles per locus and He values ranging from 0.54 to 0.88. Loci in North American mosquitoes were more variable. Cx. p. quinquefasciatus from South Carolina had five to 19 alleles per locus and He values ranging from 0.64 to 0.90, whereas Cx. p. pipiens from Massachusetts had six to 28 alleles per locus and with He values ranging from 0.65 to 0.94. All loci were associated randomly. Overall, four of nine of these new loci satisfied all six criteria for broad utility for analyzing the genetic structure of Cx. pipiens populations.
KW - Culex p. quinquefasciatus
KW - Culex pipiens complex
KW - Microsatellite loci
UR - http://www.scopus.com/inward/record.url?scp=33847732696&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=33847732696&partnerID=8YFLogxK
U2 - 10.1603/0022-2585(2007)44[145:ASOBAM]2.0.CO;2
DO - 10.1603/0022-2585(2007)44[145:ASOBAM]2.0.CO;2
M3 - Article
C2 - 17294932
AN - SCOPUS:33847732696
VL - 44
SP - 145
EP - 149
JO - Journal of Medical Entomology
JF - Journal of Medical Entomology
SN - 0022-2585
IS - 1
ER -