Abstract
Trace amounts of cashew nut protein can provoke severe allergic reactions in sensitive patients. Consequently, commercial food processors and regulatory agencies must be vigilant to prevent cashew nut cross-contamination among foods and ensure proper labeling. Toward this end, we have developed a sandwich enzyme-linked immunosorbent (ELISA) to detect the predominant cashew protein fraction (anacardein or cashew major protein, CMP) that can be extracted in aqueous buffer from food matrixes. Protein G-purified goat antiwhole cashew extract IgG and rabbit anti-CMP IgG were used as capture and secondary antibodies, respectively. Immunoadsorption against several nut and seed proteins significantly minimized the inherent cross-reactivity of these reagents. Food samples spiked with cashew flour and CMP were extracted and tested in a sandwich ELISA where standard curves were based on reactivity with CMP. The assay was optimized to detect as little as 20 ng/mL (0.02 ppm) of CMP and was successfully used to quantify CMP, and thus cashew, in various food matrixes.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 3215-3221 |
| Number of pages | 7 |
| Journal | Journal of Agricultural and Food Chemistry |
| Volume | 51 |
| Issue number | 11 |
| DOIs | |
| State | Published - May 21 2003 |
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Keywords
- Allergen
- Anacardein
- Anacardium occidentale
- Cashew
- CMP
- ELISA
- Food allergy
- Globulin
- Immunoassay
ASJC Scopus subject areas
- Agricultural and Biological Sciences (miscellaneous)
- Food Science
- Chemistry (miscellaneous)
Cite this
A sensitive sandwich ELISA for the detection of trace amounts of cashew (Anacardium occidentale L.) nut in foods. / Wei, Yanhong; Sathe, Shridhar K.; Teuber, Suzanne S; Roux, Kenneth H.
In: Journal of Agricultural and Food Chemistry, Vol. 51, No. 11, 21.05.2003, p. 3215-3221.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - A sensitive sandwich ELISA for the detection of trace amounts of cashew (Anacardium occidentale L.) nut in foods
AU - Wei, Yanhong
AU - Sathe, Shridhar K.
AU - Teuber, Suzanne S
AU - Roux, Kenneth H.
PY - 2003/5/21
Y1 - 2003/5/21
N2 - Trace amounts of cashew nut protein can provoke severe allergic reactions in sensitive patients. Consequently, commercial food processors and regulatory agencies must be vigilant to prevent cashew nut cross-contamination among foods and ensure proper labeling. Toward this end, we have developed a sandwich enzyme-linked immunosorbent (ELISA) to detect the predominant cashew protein fraction (anacardein or cashew major protein, CMP) that can be extracted in aqueous buffer from food matrixes. Protein G-purified goat antiwhole cashew extract IgG and rabbit anti-CMP IgG were used as capture and secondary antibodies, respectively. Immunoadsorption against several nut and seed proteins significantly minimized the inherent cross-reactivity of these reagents. Food samples spiked with cashew flour and CMP were extracted and tested in a sandwich ELISA where standard curves were based on reactivity with CMP. The assay was optimized to detect as little as 20 ng/mL (0.02 ppm) of CMP and was successfully used to quantify CMP, and thus cashew, in various food matrixes.
AB - Trace amounts of cashew nut protein can provoke severe allergic reactions in sensitive patients. Consequently, commercial food processors and regulatory agencies must be vigilant to prevent cashew nut cross-contamination among foods and ensure proper labeling. Toward this end, we have developed a sandwich enzyme-linked immunosorbent (ELISA) to detect the predominant cashew protein fraction (anacardein or cashew major protein, CMP) that can be extracted in aqueous buffer from food matrixes. Protein G-purified goat antiwhole cashew extract IgG and rabbit anti-CMP IgG were used as capture and secondary antibodies, respectively. Immunoadsorption against several nut and seed proteins significantly minimized the inherent cross-reactivity of these reagents. Food samples spiked with cashew flour and CMP were extracted and tested in a sandwich ELISA where standard curves were based on reactivity with CMP. The assay was optimized to detect as little as 20 ng/mL (0.02 ppm) of CMP and was successfully used to quantify CMP, and thus cashew, in various food matrixes.
KW - Allergen
KW - Anacardein
KW - Anacardium occidentale
KW - Cashew
KW - CMP
KW - ELISA
KW - Food allergy
KW - Globulin
KW - Immunoassay
UR - http://www.scopus.com/inward/record.url?scp=0038624021&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0038624021&partnerID=8YFLogxK
U2 - 10.1021/jf025977x
DO - 10.1021/jf025977x
M3 - Article
C2 - 12744645
AN - SCOPUS:0038624021
VL - 51
SP - 3215
EP - 3221
JO - Journal of Agricultural and Food Chemistry
JF - Journal of Agricultural and Food Chemistry
SN - 0021-8561
IS - 11
ER -