A sensitive sandwich ELISA for the detection of trace amounts of cashew (Anacardium occidentale L.) nut in foods

Yanhong Wei, Shridhar K. Sathe, Suzanne S Teuber, Kenneth H. Roux

Research output: Contribution to journalArticle

33 Citations (Scopus)

Abstract

Trace amounts of cashew nut protein can provoke severe allergic reactions in sensitive patients. Consequently, commercial food processors and regulatory agencies must be vigilant to prevent cashew nut cross-contamination among foods and ensure proper labeling. Toward this end, we have developed a sandwich enzyme-linked immunosorbent (ELISA) to detect the predominant cashew protein fraction (anacardein or cashew major protein, CMP) that can be extracted in aqueous buffer from food matrixes. Protein G-purified goat antiwhole cashew extract IgG and rabbit anti-CMP IgG were used as capture and secondary antibodies, respectively. Immunoadsorption against several nut and seed proteins significantly minimized the inherent cross-reactivity of these reagents. Food samples spiked with cashew flour and CMP were extracted and tested in a sandwich ELISA where standard curves were based on reactivity with CMP. The assay was optimized to detect as little as 20 ng/mL (0.02 ppm) of CMP and was successfully used to quantify CMP, and thus cashew, in various food matrixes.

Original languageEnglish (US)
Pages (from-to)3215-3221
Number of pages7
JournalJournal of Agricultural and Food Chemistry
Volume51
Issue number11
DOIs
StatePublished - May 21 2003

Fingerprint

Anacardium
Anacardium occidentale
cashew nuts
Nuts
sandwiches
nuts
Enzyme-Linked Immunosorbent Assay
enzyme-linked immunosorbent assay
Food
cashew fruit
Proteins
proteins
food matrix
Immunoglobulin G
Immunosorbents
Food Contamination
cross contamination
Labeling
Flour
Seed

Keywords

  • Allergen
  • Anacardein
  • Anacardium occidentale
  • Cashew
  • CMP
  • ELISA
  • Food allergy
  • Globulin
  • Immunoassay

ASJC Scopus subject areas

  • Agricultural and Biological Sciences (miscellaneous)
  • Food Science
  • Chemistry (miscellaneous)

Cite this

A sensitive sandwich ELISA for the detection of trace amounts of cashew (Anacardium occidentale L.) nut in foods. / Wei, Yanhong; Sathe, Shridhar K.; Teuber, Suzanne S; Roux, Kenneth H.

In: Journal of Agricultural and Food Chemistry, Vol. 51, No. 11, 21.05.2003, p. 3215-3221.

Research output: Contribution to journalArticle

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