Abstract
A high-pressure liquid chromatographic method which is capable of measuring 5-fluorouracil and 5-fluorodeoxyuridine at concentrations of 50 nM in human plasma is presented. The method employs tritium-labeled 5-fluorouracil and 5-fluorodeoxyuridine as internal standards. The fluoropyrimidines are removed from plasma by anion-exchange chromatography and are extracted into ethyl acetate. The ethyl acetate is evaporated to dryness and 5-fluorouracil and 5-fluorodeoxyuridine are chromatographed on a semipreparative μBondapak C18 column. The method is specific and sufficiently sensitive to measure the levels of 5-fluorouracil in plasma below 1 μM as found after intrahepatic or intraperitoneal drug administration.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 432-437 |
| Number of pages | 6 |
| Journal | Analytical Biochemistry |
| Volume | 106 |
| Issue number | 2 |
| State | Published - 1980 |
| Externally published | Yes |
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ASJC Scopus subject areas
- Biochemistry
- Biophysics
- Molecular Biology
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A sensitive method for determination of 5-fluorouracil and 5-fluoro-2'-deoxyuridine in human plasma by high-pressure liquid chromatography. / Buckpitt, Alan R; Boyd, M. R.
In: Analytical Biochemistry, Vol. 106, No. 2, 1980, p. 432-437.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - A sensitive method for determination of 5-fluorouracil and 5-fluoro-2'-deoxyuridine in human plasma by high-pressure liquid chromatography
AU - Buckpitt, Alan R
AU - Boyd, M. R.
PY - 1980
Y1 - 1980
N2 - A high-pressure liquid chromatographic method which is capable of measuring 5-fluorouracil and 5-fluorodeoxyuridine at concentrations of 50 nM in human plasma is presented. The method employs tritium-labeled 5-fluorouracil and 5-fluorodeoxyuridine as internal standards. The fluoropyrimidines are removed from plasma by anion-exchange chromatography and are extracted into ethyl acetate. The ethyl acetate is evaporated to dryness and 5-fluorouracil and 5-fluorodeoxyuridine are chromatographed on a semipreparative μBondapak C18 column. The method is specific and sufficiently sensitive to measure the levels of 5-fluorouracil in plasma below 1 μM as found after intrahepatic or intraperitoneal drug administration.
AB - A high-pressure liquid chromatographic method which is capable of measuring 5-fluorouracil and 5-fluorodeoxyuridine at concentrations of 50 nM in human plasma is presented. The method employs tritium-labeled 5-fluorouracil and 5-fluorodeoxyuridine as internal standards. The fluoropyrimidines are removed from plasma by anion-exchange chromatography and are extracted into ethyl acetate. The ethyl acetate is evaporated to dryness and 5-fluorouracil and 5-fluorodeoxyuridine are chromatographed on a semipreparative μBondapak C18 column. The method is specific and sufficiently sensitive to measure the levels of 5-fluorouracil in plasma below 1 μM as found after intrahepatic or intraperitoneal drug administration.
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UR - http://www.scopus.com/inward/citedby.url?scp=0019166854&partnerID=8YFLogxK
M3 - Article
VL - 106
SP - 432
EP - 437
JO - Analytical Biochemistry
JF - Analytical Biochemistry
SN - 0003-2697
IS - 2
ER -