A sensitive method for determination of 5-fluorouracil and 5-fluoro-2'-deoxyuridine in human plasma by high-pressure liquid chromatography

Alan R Buckpitt, M. R. Boyd

Research output: Contribution to journalArticle

51 Scopus citations


A high-pressure liquid chromatographic method which is capable of measuring 5-fluorouracil and 5-fluorodeoxyuridine at concentrations of 50 nM in human plasma is presented. The method employs tritium-labeled 5-fluorouracil and 5-fluorodeoxyuridine as internal standards. The fluoropyrimidines are removed from plasma by anion-exchange chromatography and are extracted into ethyl acetate. The ethyl acetate is evaporated to dryness and 5-fluorouracil and 5-fluorodeoxyuridine are chromatographed on a semipreparative μBondapak C18 column. The method is specific and sufficiently sensitive to measure the levels of 5-fluorouracil in plasma below 1 μM as found after intrahepatic or intraperitoneal drug administration.

Original languageEnglish (US)
Pages (from-to)432-437
Number of pages6
JournalAnalytical Biochemistry
Issue number2
StatePublished - 1980
Externally publishedYes


ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

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