A sensitive method for determination of 5-fluorouracil and 5-fluoro-2'-deoxyuridine in human plasma by high-pressure liquid chromatography

Alan R Buckpitt, M. R. Boyd

Research output: Contribution to journalArticle

51 Citations (Scopus)

Abstract

A high-pressure liquid chromatographic method which is capable of measuring 5-fluorouracil and 5-fluorodeoxyuridine at concentrations of 50 nM in human plasma is presented. The method employs tritium-labeled 5-fluorouracil and 5-fluorodeoxyuridine as internal standards. The fluoropyrimidines are removed from plasma by anion-exchange chromatography and are extracted into ethyl acetate. The ethyl acetate is evaporated to dryness and 5-fluorouracil and 5-fluorodeoxyuridine are chromatographed on a semipreparative μBondapak C18 column. The method is specific and sufficiently sensitive to measure the levels of 5-fluorouracil in plasma below 1 μM as found after intrahepatic or intraperitoneal drug administration.

Original languageEnglish (US)
Pages (from-to)432-437
Number of pages6
JournalAnalytical Biochemistry
Volume106
Issue number2
StatePublished - 1980
Externally publishedYes

Fingerprint

High pressure liquid chromatography
Plasma (human)
Floxuridine
Fluorouracil
High Pressure Liquid Chromatography
Plasmas
Plasma Exchange
Tritium
Chromatography
Anions
Pressure
5-fluoro-2'-deoxyuridine
Liquids
Pharmaceutical Preparations
ethyl acetate

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

@article{6c4623c873f940fcb9ffbff5fa983c84,
title = "A sensitive method for determination of 5-fluorouracil and 5-fluoro-2'-deoxyuridine in human plasma by high-pressure liquid chromatography",
abstract = "A high-pressure liquid chromatographic method which is capable of measuring 5-fluorouracil and 5-fluorodeoxyuridine at concentrations of 50 nM in human plasma is presented. The method employs tritium-labeled 5-fluorouracil and 5-fluorodeoxyuridine as internal standards. The fluoropyrimidines are removed from plasma by anion-exchange chromatography and are extracted into ethyl acetate. The ethyl acetate is evaporated to dryness and 5-fluorouracil and 5-fluorodeoxyuridine are chromatographed on a semipreparative μBondapak C18 column. The method is specific and sufficiently sensitive to measure the levels of 5-fluorouracil in plasma below 1 μM as found after intrahepatic or intraperitoneal drug administration.",
author = "Buckpitt, {Alan R} and Boyd, {M. R.}",
year = "1980",
language = "English (US)",
volume = "106",
pages = "432--437",
journal = "Analytical Biochemistry",
issn = "0003-2697",
publisher = "Academic Press Inc.",
number = "2",

}

TY - JOUR

T1 - A sensitive method for determination of 5-fluorouracil and 5-fluoro-2'-deoxyuridine in human plasma by high-pressure liquid chromatography

AU - Buckpitt, Alan R

AU - Boyd, M. R.

PY - 1980

Y1 - 1980

N2 - A high-pressure liquid chromatographic method which is capable of measuring 5-fluorouracil and 5-fluorodeoxyuridine at concentrations of 50 nM in human plasma is presented. The method employs tritium-labeled 5-fluorouracil and 5-fluorodeoxyuridine as internal standards. The fluoropyrimidines are removed from plasma by anion-exchange chromatography and are extracted into ethyl acetate. The ethyl acetate is evaporated to dryness and 5-fluorouracil and 5-fluorodeoxyuridine are chromatographed on a semipreparative μBondapak C18 column. The method is specific and sufficiently sensitive to measure the levels of 5-fluorouracil in plasma below 1 μM as found after intrahepatic or intraperitoneal drug administration.

AB - A high-pressure liquid chromatographic method which is capable of measuring 5-fluorouracil and 5-fluorodeoxyuridine at concentrations of 50 nM in human plasma is presented. The method employs tritium-labeled 5-fluorouracil and 5-fluorodeoxyuridine as internal standards. The fluoropyrimidines are removed from plasma by anion-exchange chromatography and are extracted into ethyl acetate. The ethyl acetate is evaporated to dryness and 5-fluorouracil and 5-fluorodeoxyuridine are chromatographed on a semipreparative μBondapak C18 column. The method is specific and sufficiently sensitive to measure the levels of 5-fluorouracil in plasma below 1 μM as found after intrahepatic or intraperitoneal drug administration.

UR - http://www.scopus.com/inward/record.url?scp=0019166854&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0019166854&partnerID=8YFLogxK

M3 - Article

VL - 106

SP - 432

EP - 437

JO - Analytical Biochemistry

JF - Analytical Biochemistry

SN - 0003-2697

IS - 2

ER -