Abstract
A high-pressure liquid chromatographic method which is capable of measuring 5-fluorouracil and 5-fluorodeoxyuridine at concentrations of 50 nM in human plasma is presented. The method employs tritium-labeled 5-fluorouracil and 5-fluorodeoxyuridine as internal standards. The fluoropyrimidines are removed from plasma by anion-exchange chromatography and are extracted into ethyl acetate. The ethyl acetate is evaporated to dryness and 5-fluorouracil and 5-fluorodeoxyuridine are chromatographed on a semipreparative μBondapak C18 column. The method is specific and sufficiently sensitive to measure the levels of 5-fluorouracil in plasma below 1 μM as found after intrahepatic or intraperitoneal drug administration.
Original language | English (US) |
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Pages (from-to) | 432-437 |
Number of pages | 6 |
Journal | Analytical Biochemistry |
Volume | 106 |
Issue number | 2 |
State | Published - 1980 |
Externally published | Yes |
ASJC Scopus subject areas
- Biochemistry
- Biophysics
- Molecular Biology