A Sensitive Class Specific Immunoassay for the Detection of Pyrethroid Metabolites in Human Urine

Guomin Shan, Huazhang Huang, Donald W. Stoutamire, Shirley J. Gee, Gabriele Leng, Bruce D. Hammock

Research output: Contribution to journalArticle

64 Citations (Scopus)

Abstract

The pyrethroids are one of the most heavily used insecticide classes in the world. It is important to develop sensitive and rapid analytical techniques for environmental monitoring and assessment of human exposure to these compounds. Because major pyrethroids contain a phenoxybenzyl group and phenoxybenzoic acid (PBA) is a common metabolite form or intermediate, PBA might be used as a biomarker of human exposure to pyrethroids. A sensitive and selective immunoassay for the common pyrethroid metabolite PBA was developed. Rabbits were immunized with 3-[4-(3-carboxyphenoxy)phenoxy] N-thyroglobulin ethylamine. All sera were screened against numerous coating antigens. The assay with the least interference and the best sensitivity was optimized and characterized. The average IC50 for free PBA was 1.65 ng/mL. No cross-reactivity was measured to parent pyrethroids and other metabolites. Urine matrix effects can be eliminated by simple dilution. Results from urine samples from exposed workers suggest that this PBA immunoassay might be suitable as a monitoring tool for human exposure to pyrethroids.

Original languageEnglish (US)
Pages (from-to)218-225
Number of pages8
JournalChemical Research in Toxicology
Volume17
Issue number2
DOIs
StatePublished - Feb 2004

Fingerprint

Pyrethrins
Metabolites
Immunoassay
Urine
Acids
Monitoring
Thyroglobulin
Environmental Monitoring
Biomarkers
Insecticides
Dilution
Inhibitory Concentration 50
Assays
Rabbits
Antigens
Coatings
Serum

ASJC Scopus subject areas

  • Drug Discovery
  • Organic Chemistry
  • Chemistry(all)
  • Toxicology
  • Health, Toxicology and Mutagenesis

Cite this

A Sensitive Class Specific Immunoassay for the Detection of Pyrethroid Metabolites in Human Urine. / Shan, Guomin; Huang, Huazhang; Stoutamire, Donald W.; Gee, Shirley J.; Leng, Gabriele; Hammock, Bruce D.

In: Chemical Research in Toxicology, Vol. 17, No. 2, 02.2004, p. 218-225.

Research output: Contribution to journalArticle

Shan, Guomin ; Huang, Huazhang ; Stoutamire, Donald W. ; Gee, Shirley J. ; Leng, Gabriele ; Hammock, Bruce D. / A Sensitive Class Specific Immunoassay for the Detection of Pyrethroid Metabolites in Human Urine. In: Chemical Research in Toxicology. 2004 ; Vol. 17, No. 2. pp. 218-225.
@article{62e6e756aee548518ec76cc6728f778f,
title = "A Sensitive Class Specific Immunoassay for the Detection of Pyrethroid Metabolites in Human Urine",
abstract = "The pyrethroids are one of the most heavily used insecticide classes in the world. It is important to develop sensitive and rapid analytical techniques for environmental monitoring and assessment of human exposure to these compounds. Because major pyrethroids contain a phenoxybenzyl group and phenoxybenzoic acid (PBA) is a common metabolite form or intermediate, PBA might be used as a biomarker of human exposure to pyrethroids. A sensitive and selective immunoassay for the common pyrethroid metabolite PBA was developed. Rabbits were immunized with 3-[4-(3-carboxyphenoxy)phenoxy] N-thyroglobulin ethylamine. All sera were screened against numerous coating antigens. The assay with the least interference and the best sensitivity was optimized and characterized. The average IC50 for free PBA was 1.65 ng/mL. No cross-reactivity was measured to parent pyrethroids and other metabolites. Urine matrix effects can be eliminated by simple dilution. Results from urine samples from exposed workers suggest that this PBA immunoassay might be suitable as a monitoring tool for human exposure to pyrethroids.",
author = "Guomin Shan and Huazhang Huang and Stoutamire, {Donald W.} and Gee, {Shirley J.} and Gabriele Leng and Hammock, {Bruce D.}",
year = "2004",
month = "2",
doi = "10.1021/tx034220c",
language = "English (US)",
volume = "17",
pages = "218--225",
journal = "Chemical Research in Toxicology",
issn = "0893-228X",
publisher = "American Chemical Society",
number = "2",

}

TY - JOUR

T1 - A Sensitive Class Specific Immunoassay for the Detection of Pyrethroid Metabolites in Human Urine

AU - Shan, Guomin

AU - Huang, Huazhang

AU - Stoutamire, Donald W.

AU - Gee, Shirley J.

AU - Leng, Gabriele

AU - Hammock, Bruce D.

PY - 2004/2

Y1 - 2004/2

N2 - The pyrethroids are one of the most heavily used insecticide classes in the world. It is important to develop sensitive and rapid analytical techniques for environmental monitoring and assessment of human exposure to these compounds. Because major pyrethroids contain a phenoxybenzyl group and phenoxybenzoic acid (PBA) is a common metabolite form or intermediate, PBA might be used as a biomarker of human exposure to pyrethroids. A sensitive and selective immunoassay for the common pyrethroid metabolite PBA was developed. Rabbits were immunized with 3-[4-(3-carboxyphenoxy)phenoxy] N-thyroglobulin ethylamine. All sera were screened against numerous coating antigens. The assay with the least interference and the best sensitivity was optimized and characterized. The average IC50 for free PBA was 1.65 ng/mL. No cross-reactivity was measured to parent pyrethroids and other metabolites. Urine matrix effects can be eliminated by simple dilution. Results from urine samples from exposed workers suggest that this PBA immunoassay might be suitable as a monitoring tool for human exposure to pyrethroids.

AB - The pyrethroids are one of the most heavily used insecticide classes in the world. It is important to develop sensitive and rapid analytical techniques for environmental monitoring and assessment of human exposure to these compounds. Because major pyrethroids contain a phenoxybenzyl group and phenoxybenzoic acid (PBA) is a common metabolite form or intermediate, PBA might be used as a biomarker of human exposure to pyrethroids. A sensitive and selective immunoassay for the common pyrethroid metabolite PBA was developed. Rabbits were immunized with 3-[4-(3-carboxyphenoxy)phenoxy] N-thyroglobulin ethylamine. All sera were screened against numerous coating antigens. The assay with the least interference and the best sensitivity was optimized and characterized. The average IC50 for free PBA was 1.65 ng/mL. No cross-reactivity was measured to parent pyrethroids and other metabolites. Urine matrix effects can be eliminated by simple dilution. Results from urine samples from exposed workers suggest that this PBA immunoassay might be suitable as a monitoring tool for human exposure to pyrethroids.

UR - http://www.scopus.com/inward/record.url?scp=1342289370&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=1342289370&partnerID=8YFLogxK

U2 - 10.1021/tx034220c

DO - 10.1021/tx034220c

M3 - Article

C2 - 14967009

AN - SCOPUS:1342289370

VL - 17

SP - 218

EP - 225

JO - Chemical Research in Toxicology

JF - Chemical Research in Toxicology

SN - 0893-228X

IS - 2

ER -