A screening method for isolating DNA repair-deficient mutants of CHO cells

Larry H. Thompson, Jaime S. Rubin, James E. Cleaver, Gordon F. Whitmore, Kerry Brookman

Research output: Contribution to journalArticle

236 Scopus citations

Abstract

A simple procedure for isolating mutagen-sensitive clones of CHO cells was developed and applied in mutant hunts in which colonies were screened for hypersensitivity to killing by ultraviolet radiation (UV), ethyl methanesulfonate (EMS), or mitomycin C (MMC). Each of two UV-sensitive clones studied in detail had a D37 dose of 1.0 J/m2 compared to 7.0 J/m2 for the wild-type cells, and each was shown to have no detectable repair replication following exposure to UV doses of up to 26 J/m2. Although these mutants resemble xeroderma pigmentosum human mutants with respect to their repair defect and cross-sensitivity to the carcinogen 4-nitroquinoline-1-oxide, one of two clones (UV-20) is characterized by extreme hypersensitivity to MMC (80-fold as compared to the wild type). Clones having hypersensitivity to alkylating agents, but not UV, were obtained using MMC and EMS. In the latter case the two clones had significantly increased sensitivity to the killing action of60Co γ-rays.

Original languageEnglish (US)
Pages (from-to)391-405
Number of pages15
JournalSomatic Cell Genetics
Volume6
Issue number3
DOIs
StatePublished - May 1980
Externally publishedYes

ASJC Scopus subject areas

  • Genetics
  • Medicine(all)

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    Thompson, L. H., Rubin, J. S., Cleaver, J. E., Whitmore, G. F., & Brookman, K. (1980). A screening method for isolating DNA repair-deficient mutants of CHO cells. Somatic Cell Genetics, 6(3), 391-405. https://doi.org/10.1007/BF01542791