A salmonella regulator modulates intestinal colonization and use of phosphonoacetic acid

Johanna R. Elfenbein, Leigh A. Knodler, Allison R. Schaeffer, Franziska Faber, Andreas J Baumler, Helene L. Andrews-Polymenis

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Many microorganisms produce phosphonates, molecules characterized by stable carbon-phosphorus bonds that store phosphorus or act as antimicrobials. The role of phosphonates in the marine biosphere is well characterized but the role of these molecules in the intestine is poorly understood. Salmonella enterica uses its virulence factors to influence the host immune response to compete with the host and normal microflora for nutrients. Salmonella cannot produce phosphonates but encodes the enzymes to use them suggesting that it is exposed to phosphonates during its life cycle. The role of phosphonates during enteric salmonellosis is unexplored. We have previously shown that STM3602, encoding a putative regulator of phosphonate metabolism, is needed for colonization in calves. Here, we report that the necessity of STM3602 in colonization of the murine intestine results from multiple factors. STM3602 is needed for full activation of the type-3 secretion system-1 and for optimal invasion of epithelial cells. The ∆STM3602 mutant grows poorly in phosphonoacetic acid (PA) as the sole phosphorus source, but can use 2-aminoethylphosphonate. PhnA, an enzyme required for PA breakdown, is not controlled by STM3602 suggesting an additional mechanism for utilization of PA in S. Typhimurium. Finally, the requirement of STM3602 for intestinal colonization differs depending on the composition of the microflora. Our data suggest that STM3602 has multiple regulatory targets that are necessary for survival within the microbial community in the intestine. Determination of the members of the STM3602 regulon may illuminate new pathways needed for colonization of the host.

Original languageEnglish (US)
Article number69
JournalFrontiers in cellular and infection microbiology
Volume7
Issue numberMAR
DOIs
StatePublished - Mar 15 2017

Fingerprint

Phosphonoacetic Acid
Organophosphonates
Salmonella
Phosphorus
Intestines
Regulon
Salmonella enterica
Salmonella Infections
Virulence Factors
Enzymes
Life Cycle Stages
Carbon
Epithelial Cells
Food

Keywords

  • Infection
  • Mice
  • Phosphonates
  • Phosphonoacetic acid
  • Salmonella

ASJC Scopus subject areas

  • Microbiology
  • Immunology
  • Medicine(all)
  • Microbiology (medical)
  • Infectious Diseases

Cite this

A salmonella regulator modulates intestinal colonization and use of phosphonoacetic acid. / Elfenbein, Johanna R.; Knodler, Leigh A.; Schaeffer, Allison R.; Faber, Franziska; Baumler, Andreas J; Andrews-Polymenis, Helene L.

In: Frontiers in cellular and infection microbiology, Vol. 7, No. MAR, 69, 15.03.2017.

Research output: Contribution to journalArticle

Elfenbein, Johanna R. ; Knodler, Leigh A. ; Schaeffer, Allison R. ; Faber, Franziska ; Baumler, Andreas J ; Andrews-Polymenis, Helene L. / A salmonella regulator modulates intestinal colonization and use of phosphonoacetic acid. In: Frontiers in cellular and infection microbiology. 2017 ; Vol. 7, No. MAR.
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