A ruthenium red-sensitive mechanism mediates in part the protective mesenteric hyperemia after intraduodenal acidification in rats

K. Seno, F. Iwata, K. Lam, Joseph Leung, F. W. Leung

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1 Scopus citations

Abstract

We tested the hypothesis that capsazepine [a selective competitive receptor antagonist of capsaicin] and ruthenium red (RR) [an inhibitor of capsaicin-sensitive cation-selective ion channel] will attenuate the mesenteric vasodilatory effect of intraduodenal (ID) HCl and increase duodenal villous injury. I. Subcutaneous 100 μmol/kg capsazepine or ID 0.1% or 0.2% RR was given. Then, ID 640 μM capsaicin or 0.1 N HCl was administered. II. The duodenal villous damage induced by perfusion of 0.1 N HCl after ID RR was examined. The villous damage was graded from 0 (no damage) to 4 (deep damage). The lesion score equaled the sum of all grades divided by the number of villi. Results: mean ± SEM. a vs respective vehicle, p < 0.05. Study I ID Capsaicin ID HCl Lesion Scores Treatment n Blood Flow Change Blood Flow Change as bolus HCl Vehicle (DMSO) 6 15.1 ± 1.5 (%) 15.5 ± 2.0 (%) 0.68 ± 0.04 Capsazepine 6 9.8 ± 1.3a 17.5 ± 4.1 0.64 ± 0.15 Vehicle (saline) 6 17.8 ± 1.6 19.3 ± 3.5 0.72 ± 0.14 0.1% RR 6 12.4 ± 1.93 11.9 ± 1.9 a 1.00 ± 0.13 0.2% RR 6 9.5 ± 1.3a 10.2 1.6a 0.89 ± 0.12 Study II Frequency of Lesion Grades Lesion Treatment n 0 1 2 3 4 Scores Vehicle 10 5± 1 18 ± 2 30 ± 3 17 ± 2 0 1.85 ± 0.08 0.1% RR 10 3 ± 1 15 ± 2 27 ± 3 24 ± 2a 1 ± 0 2.08 ± 0.06a Conclusions: These data suggest that a RR but not capsazepine-sensitive mechanism mediates the protective mesenteric hyperemia after ID acidification in rats.

Original languageEnglish (US)
JournalJournal of Investigative Medicine
Volume44
Issue number1
StatePublished - 1996

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

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