A role of IgE in allergic asthma: IgE enhances airway inflammation in a mouse model

R. Zuberi, J. Apgar, S. S. Chen, Fu-Tong Liu

Research output: Contribution to journalArticle

Abstract

We have studied the role of IgE in allergic asthma by using a mouse model of airway inflammation. BALB/c mice were primarily immunized with ovalbumin ( OVA ) in alum administered intraperitoneally; these mice were then challenged after 14 days with OVA in phosphate-buffered saline ( PBS ) given intranasally or through aerosol for 30 min, once a day for 3 or 6 days. Control mice received PBS only. Three or 24 hours after last challenge, the lung airways of these mice were lavaged via tracheal cannulation and bronchoalveolar lavage ( BAL ) fluid was collected. We discovered that significantly higher levels of IgE were present in BAL fluid from the antigen-challenged mice as compared to the control mice. Measured amount of IgE in antigen-challenged mice was 25 - 32 ng/ml BAL fluid, of which over 65% was antigen-specific. We, then, investigated the possible role of IgE in the airway inflammatory response. OVA-sensitized mice were challenged with anti-DNP IgE, TNP.OVA or TNP.OVA complexed to anti-DNP IgE. We found that mice challenged with IgE immune complexes developed significantly higher numbers of eosinophils and secreted higher amounts of IL-4 in their airways. These results suggest a hitherto unraveled role of IgE in antigen presentation during allergic airway inflammation. Antigen-specific IgE in airway secretions may contribute to antigen capturing and perpetuation of the allergic inflammatory responses.

Original languageEnglish (US)
JournalFASEB Journal
Volume12
Issue number5
StatePublished - Mar 20 1998
Externally publishedYes

Fingerprint

asthma
Immunoglobulin E
Asthma
inflammation
animal models
Inflammation
ovalbumin
Ovalbumin
mice
Antigens
antigens
Bronchoalveolar Lavage Fluid
Fluids
Phosphates
phosphates
antigen presentation
antigen-antibody complex
alum
interleukin-4
eosinophils

ASJC Scopus subject areas

  • Agricultural and Biological Sciences (miscellaneous)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry
  • Cell Biology

Cite this

Zuberi, R., Apgar, J., Chen, S. S., & Liu, F-T. (1998). A role of IgE in allergic asthma: IgE enhances airway inflammation in a mouse model. FASEB Journal, 12(5).

A role of IgE in allergic asthma : IgE enhances airway inflammation in a mouse model. / Zuberi, R.; Apgar, J.; Chen, S. S.; Liu, Fu-Tong.

In: FASEB Journal, Vol. 12, No. 5, 20.03.1998.

Research output: Contribution to journalArticle

Zuberi, R, Apgar, J, Chen, SS & Liu, F-T 1998, 'A role of IgE in allergic asthma: IgE enhances airway inflammation in a mouse model', FASEB Journal, vol. 12, no. 5.
Zuberi, R. ; Apgar, J. ; Chen, S. S. ; Liu, Fu-Tong. / A role of IgE in allergic asthma : IgE enhances airway inflammation in a mouse model. In: FASEB Journal. 1998 ; Vol. 12, No. 5.
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