A role for the spectrin superfamily member syne-1 and kinesin II in cytokinesis

Jun Fan, Kenneth A Beck

Research output: Contribution to journalArticle

82 Scopus citations

Abstract

Expression of a dominant negative fragment of the spectrin family member Syne-1 causes an accumulation of binucleate cells, suggesting a role for this protein in cytokinesis. An association of this fragment with the C-terminal tail domain of the kinesin II subunit KIF3B was identified by yeast two-hybrid and co-precipitation assays, suggesting that the role of Syne-1 in cytokinesis involves an interaction with kinesin II. In support of this we found that (1) expression of KIF3B tail domain also gives rise to multinucleate cells, (2) both Syne-1 and KIF3B localize to the central spindle and midbody during cytokinesis in a detergent resistant and ATP sensitive manner and (3) Syne-1 localization is blocked by expression of KIF3B tail. Also, membrane vesicles containing syntaxin associate with the spindle midbody with identical properties. We conclude that Syne-1 and KIF3B function together in cytokinesis by facilitating the accumulation of membrane vesicles at the spindle midbody.

Original languageEnglish (US)
Pages (from-to)619-629
Number of pages11
JournalJournal of Cell Science
Volume117
Issue number4
DOIs
StatePublished - Feb 1 2004

Keywords

  • Cytokinesis
  • Golgi
  • Kinesin
  • Mitosis
  • Spectrin
  • Vesicle transport

ASJC Scopus subject areas

  • Cell Biology

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