A role for the Saccharomyces cerevisiae ATX1 gene in copper trafficking and iron transport

Su Ju Lin, Robert A. Pufahl, Andrew Dancis, Thomas V. O'Halloran, Valeria Cizewski Culotta

Research output: Contribution to journalArticle

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Abstract

The ATX1 gene of Saccharomyces cerevisiae was originally identified as a multi-copy suppressor of oxidative damage in yeast lacking superoxide dismutase. We now provide evidence that Atx1p helps deliver copper to the copper requiring oxidase Fet3p involved in iron uptake. atx1Δ null mutants are iron-deficient and are defective in the high affinity uptake of iron. These defects due to ATX1 inactivation are rescued by copper treatment, and the same has been reported for strains lacking either the cell surface copper transporter, Ctr1p, or the putative copper transporter in the secretory pathway, Ccc2p. Atx1p localizes to the cytosol, and our studies indicate that it functions as a carrier for copper that delivers the metal from the cell surface Ctr1p to Ccc2p and then to Fet3p within the secretory pathway. The iron deficiency of atx1 mutants is augmented by mutations in END3 blocking endocytosis, suggesting that a parallel pathway for intracellular copper trafficking is mediated by endocytosis. As additional evidence for the role of Atx1p in iron metabolism, we find that the gene is induced by the same iron-sensing trans-activator, Aft1p, that regulates CCC2 and FET3.

Original languageEnglish (US)
Pages (from-to)9215-9220
Number of pages6
JournalJournal of Biological Chemistry
Volume272
Issue number14
DOIs
StatePublished - Apr 4 1997
Externally publishedYes

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Yeast
Saccharomyces cerevisiae
Copper
Iron
Genes
Secretory Pathway
Endocytosis
Trans-Activators
Cytosol
Superoxide Dismutase
Metabolism
Yeasts
Metals
Oxidoreductases
Mutation
Defects

ASJC Scopus subject areas

  • Biochemistry

Cite this

Lin, S. J., Pufahl, R. A., Dancis, A., O'Halloran, T. V., & Culotta, V. C. (1997). A role for the Saccharomyces cerevisiae ATX1 gene in copper trafficking and iron transport. Journal of Biological Chemistry, 272(14), 9215-9220. https://doi.org/10.1074/jbc.272.14.9215

A role for the Saccharomyces cerevisiae ATX1 gene in copper trafficking and iron transport. / Lin, Su Ju; Pufahl, Robert A.; Dancis, Andrew; O'Halloran, Thomas V.; Culotta, Valeria Cizewski.

In: Journal of Biological Chemistry, Vol. 272, No. 14, 04.04.1997, p. 9215-9220.

Research output: Contribution to journalArticle

Lin, Su Ju ; Pufahl, Robert A. ; Dancis, Andrew ; O'Halloran, Thomas V. ; Culotta, Valeria Cizewski. / A role for the Saccharomyces cerevisiae ATX1 gene in copper trafficking and iron transport. In: Journal of Biological Chemistry. 1997 ; Vol. 272, No. 14. pp. 9215-9220.
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