A Role for Piwi and piRNAs in Germ Cell Maintenance and Transposon Silencing in Zebrafish

Saskia Houwing, Leonie M. Kamminga, Eugene Berezikov, Daniela Cronembold, Angélique Girard, Hans van den Elst, Dmitri V. Filippov, Heiko Blaser, Erez Raz, Cecilia B. Moens, Ronald H A Plasterk, Gregory J. Hannon, Bruce W. Draper, René F. Ketting

Research output: Contribution to journalArticle

624 Citations (Scopus)

Abstract

Piwi proteins specify an animal-specific subclass of the Argonaute family that, in vertebrates, is specifically expressed in germ cells. We demonstrate that zebrafish Piwi (Ziwi) is expressed in both the male and the female gonad and is a component of a germline-specifying structure called nuage. Loss of Ziwi function results in a progressive loss of germ cells due to apoptosis during larval development. In animals that have reduced Ziwi function, germ cells are maintained but display abnormal levels of apoptosis in adults. In mammals, Piwi proteins associate with approximately 29-nucleotide-long, testis-specific RNA molecules called piRNAs. Here we show that zebrafish piRNAs are present in both ovary and testis. Many of these are derived from transposons, implicating a role for piRNAs in the silencing of repetitive elements in vertebrates. Furthermore, we show that piRNAs are Dicer independent and that their 3′ end likely carries a 2′O-Methyl modification.

Original languageEnglish (US)
Pages (from-to)69-82
Number of pages14
JournalCell
Volume129
Issue number1
DOIs
StatePublished - Apr 6 2007

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Zebrafish
Germ Cells
Cells
Maintenance
Animals
Apoptosis
Vertebrates
Testis
Mammals
Transcriptional Silencer Elements
Gonads
Proteins
Nucleotides
RNA
Ovary
Molecules

ASJC Scopus subject areas

  • Cell Biology
  • Molecular Biology

Cite this

Houwing, S., Kamminga, L. M., Berezikov, E., Cronembold, D., Girard, A., van den Elst, H., ... Ketting, R. F. (2007). A Role for Piwi and piRNAs in Germ Cell Maintenance and Transposon Silencing in Zebrafish. Cell, 129(1), 69-82. https://doi.org/10.1016/j.cell.2007.03.026

A Role for Piwi and piRNAs in Germ Cell Maintenance and Transposon Silencing in Zebrafish. / Houwing, Saskia; Kamminga, Leonie M.; Berezikov, Eugene; Cronembold, Daniela; Girard, Angélique; van den Elst, Hans; Filippov, Dmitri V.; Blaser, Heiko; Raz, Erez; Moens, Cecilia B.; Plasterk, Ronald H A; Hannon, Gregory J.; Draper, Bruce W.; Ketting, René F.

In: Cell, Vol. 129, No. 1, 06.04.2007, p. 69-82.

Research output: Contribution to journalArticle

Houwing, S, Kamminga, LM, Berezikov, E, Cronembold, D, Girard, A, van den Elst, H, Filippov, DV, Blaser, H, Raz, E, Moens, CB, Plasterk, RHA, Hannon, GJ, Draper, BW & Ketting, RF 2007, 'A Role for Piwi and piRNAs in Germ Cell Maintenance and Transposon Silencing in Zebrafish', Cell, vol. 129, no. 1, pp. 69-82. https://doi.org/10.1016/j.cell.2007.03.026
Houwing S, Kamminga LM, Berezikov E, Cronembold D, Girard A, van den Elst H et al. A Role for Piwi and piRNAs in Germ Cell Maintenance and Transposon Silencing in Zebrafish. Cell. 2007 Apr 6;129(1):69-82. https://doi.org/10.1016/j.cell.2007.03.026
Houwing, Saskia ; Kamminga, Leonie M. ; Berezikov, Eugene ; Cronembold, Daniela ; Girard, Angélique ; van den Elst, Hans ; Filippov, Dmitri V. ; Blaser, Heiko ; Raz, Erez ; Moens, Cecilia B. ; Plasterk, Ronald H A ; Hannon, Gregory J. ; Draper, Bruce W. ; Ketting, René F. / A Role for Piwi and piRNAs in Germ Cell Maintenance and Transposon Silencing in Zebrafish. In: Cell. 2007 ; Vol. 129, No. 1. pp. 69-82.
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