A role for lectin interactions during human neutrophil aggregation

Yvan P. Rochon, Scott I. Simon, Eric B. Lynam, Larry A. Sklar

Research output: Contribution to journalArticle

42 Citations (Scopus)

Abstract

We have recently reported that neutrophil aggregation is dependent on both L-selectin and the β2-integrin Mac-1, raising the possibility that carbohydrate interactions play a role in aggregation. We used mono- and polysaccharides known to inhibit L-selectin-dependent adhesion of lymphocytes to high endothelial venules to test whether these carbohydrates could inhibit neutrophil aggregation. Similar types and concentrations of carbohydrates found by others to inhibit lymphocyte adhesion were effective in blocking neutrophil aggregation. Thus, nanomolar concentrations of the polysaccharides dextran sulfate (m.w. 500,000) and fucoidan inhibited aggregation, whereas dermatan sulfate, α-carrageenan, and dextran sulfate (m.w. 5,000) showed no inhibition. All of the phosphorylated monosaccharides tested inhibited aggregation with ED50 values between 8 and 17 mM, the most potent being mannose-6-phosphate and fucose-1-phosphate. The nonphosphorylated monosaccharides glucose and fucose were noninhibitory. The inhibitory effects of fucoidan or dextran sulfate (m.w. 500,000) did not appear to be due to altered regulation of L-selectin after stimulation because fucoidan reduced the rate of L-selectin shedding, whereas dextran sulfate had no effect compared with control. Neither carbohydrate inhibited the binding of formyl peptide to its receptor. However, carbohydrates were able to compete with mAb binding to a number of known leukocyte adhesion proteins. We used endotoxin pretreatment to create L-selectin-deficient neutrophils to study the minimum adhesive requirements for aggregation using two-color fluorescence flow cytometry. Our results implicate a lectinlike contribution to neutrophil aggregation, and suggest that L-selectin is the molecule that mediates the carbohydrate-dependent adhesive event.

Original languageEnglish (US)
Pages (from-to)1385-1393
Number of pages9
JournalJournal of Immunology
Volume152
Issue number3
StatePublished - Feb 1 1994
Externally publishedYes

Fingerprint

L-Selectin
Lectins
Dextran Sulfate
Neutrophils
Carbohydrates
Monosaccharides
Adhesives
Polysaccharides
Lymphocytes
Dermatan Sulfate
Fucose
Venules
Carrageenan
Leukocyte Count
Endotoxins
Integrins
Flow Cytometry
Color
Fluorescence
Glucose

ASJC Scopus subject areas

  • Immunology

Cite this

Rochon, Y. P., Simon, S. I., Lynam, E. B., & Sklar, L. A. (1994). A role for lectin interactions during human neutrophil aggregation. Journal of Immunology, 152(3), 1385-1393.

A role for lectin interactions during human neutrophil aggregation. / Rochon, Yvan P.; Simon, Scott I.; Lynam, Eric B.; Sklar, Larry A.

In: Journal of Immunology, Vol. 152, No. 3, 01.02.1994, p. 1385-1393.

Research output: Contribution to journalArticle

Rochon, YP, Simon, SI, Lynam, EB & Sklar, LA 1994, 'A role for lectin interactions during human neutrophil aggregation', Journal of Immunology, vol. 152, no. 3, pp. 1385-1393.
Rochon YP, Simon SI, Lynam EB, Sklar LA. A role for lectin interactions during human neutrophil aggregation. Journal of Immunology. 1994 Feb 1;152(3):1385-1393.
Rochon, Yvan P. ; Simon, Scott I. ; Lynam, Eric B. ; Sklar, Larry A. / A role for lectin interactions during human neutrophil aggregation. In: Journal of Immunology. 1994 ; Vol. 152, No. 3. pp. 1385-1393.
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