A role for Gle1, a regulator of DEAD-box RNA helicases, at centrosomes and basal bodies

Li-En Jao, Abdalla Akef, Susan R. Wente

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Control of organellar assembly and function is critical to eukaryotic homeostasis and survival. Gle1 is a highly conserved regulator of RNA-dependent DEAD-box ATPase proteins, with critical roles in both mRNA export and translation. In addition to its well-defined interaction with nuclear pore complexes, here we find that Gle1 is enriched at the centrosome and basal body. Gle1 assembles into the toroid-shaped pericentriolar material around the mother centriole. Reduced Gle1 levels are correlated with decreased pericentrin localization at the centrosome and microtubule organization defects. Of importance, these alterations in centrosome integrity do not result from loss of mRNA export. Examination of the Kupffer's vesicle in Gle1-depleted zebrafish revealed compromised ciliary beating and developmental defects. We propose that Gle1 assembly into the pericentriolar material positions the DEAD-box protein regulator to function in localized mRNA metabolism required for prop-er centrosome function.

Original languageEnglish (US)
Pages (from-to)120-127
Number of pages8
JournalMolecular Biology of the Cell
Volume28
Issue number1
DOIs
StatePublished - Jan 1 2017
Externally publishedYes

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DEAD-box RNA Helicases
Basal Bodies
Centrosome
Centrioles
Nuclear Pore
Messenger RNA
Protein Biosynthesis
Zebrafish
Microtubules
Adenosine Triphosphatases
Proteins
Homeostasis
RNA

ASJC Scopus subject areas

  • Molecular Biology
  • Cell Biology

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A role for Gle1, a regulator of DEAD-box RNA helicases, at centrosomes and basal bodies. / Jao, Li-En; Akef, Abdalla; Wente, Susan R.

In: Molecular Biology of the Cell, Vol. 28, No. 1, 01.01.2017, p. 120-127.

Research output: Contribution to journalArticle

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