A reactive peptidic linker for self-assembling hybrid quantum dot-DNA bioconjugates

Igor L. Medintz, Lorenzo Berti, Thomas Pons, Amy F. Grimes, Douglas S. English, Andrea Alessandrini, Paolo Facci, Hedi Mattoussi

Research output: Contribution to journalArticle

166 Citations (Scopus)

Abstract

Self-assembly of proteins, peptides, DNA, and other biomolecules to semiconductor quantum dots (QD) is an attractive bioconjugation route that can circumvent many of the problems associated with covalent chemistry and subsequent purification. Polyhistidine sequences have been shown to facilitate self-assembly of proteins and peptides to ZnS-overcoated CdSe QDs via complexation to unoccupied coordination metal sites on the nanocrystal surface. We describe the synthesis and characterization of a thiol-reactive hexahistidine peptidic linker that can be chemically attached to thiolated-DNA oligomers and mediate their self-assembly to CdSe-ZnS core-shell QDs. The self-assembly of hexahistidine-appended DNA to QDs is probed with gel electrophoresis and fluorescence resonance energy transfer techniques, and the results confirm high-affinity conjugate formation with control over the average molar ratio of DNA assembled per QD. To demonstrate the potential of this reactive peptide linker strategy, a prototype QD-DNA-dye molecular beacon is self-assembled and tested against both specific and nonspecific target DNAs. This conjugation route is potentially versatile, as altering the reactivity of the peptide linker may allow targeting of different functional groups such as amines and facilitate self-assembly of other nanoparticle-biomolecule structures.

Original languageEnglish (US)
Pages (from-to)1741-1748
Number of pages8
JournalNano Letters
Volume7
Issue number6
DOIs
StatePublished - Jun 2007
Externally publishedYes

Fingerprint

assembling
Semiconductor quantum dots
His-His-His-His-His-His
DNA
deoxyribonucleic acid
Self assembly
quantum dots
self assembly
Peptides
peptides
Biomolecules
routes
proteins
Proteins
beacons
resonance fluorescence
electrophoresis
Electrophoresis
Complexation
conjugation

ASJC Scopus subject areas

  • Materials Science(all)
  • Electronic, Optical and Magnetic Materials
  • Chemistry (miscellaneous)

Cite this

Medintz, I. L., Berti, L., Pons, T., Grimes, A. F., English, D. S., Alessandrini, A., ... Mattoussi, H. (2007). A reactive peptidic linker for self-assembling hybrid quantum dot-DNA bioconjugates. Nano Letters, 7(6), 1741-1748. https://doi.org/10.1021/nl070782v

A reactive peptidic linker for self-assembling hybrid quantum dot-DNA bioconjugates. / Medintz, Igor L.; Berti, Lorenzo; Pons, Thomas; Grimes, Amy F.; English, Douglas S.; Alessandrini, Andrea; Facci, Paolo; Mattoussi, Hedi.

In: Nano Letters, Vol. 7, No. 6, 06.2007, p. 1741-1748.

Research output: Contribution to journalArticle

Medintz, IL, Berti, L, Pons, T, Grimes, AF, English, DS, Alessandrini, A, Facci, P & Mattoussi, H 2007, 'A reactive peptidic linker for self-assembling hybrid quantum dot-DNA bioconjugates', Nano Letters, vol. 7, no. 6, pp. 1741-1748. https://doi.org/10.1021/nl070782v
Medintz IL, Berti L, Pons T, Grimes AF, English DS, Alessandrini A et al. A reactive peptidic linker for self-assembling hybrid quantum dot-DNA bioconjugates. Nano Letters. 2007 Jun;7(6):1741-1748. https://doi.org/10.1021/nl070782v
Medintz, Igor L. ; Berti, Lorenzo ; Pons, Thomas ; Grimes, Amy F. ; English, Douglas S. ; Alessandrini, Andrea ; Facci, Paolo ; Mattoussi, Hedi. / A reactive peptidic linker for self-assembling hybrid quantum dot-DNA bioconjugates. In: Nano Letters. 2007 ; Vol. 7, No. 6. pp. 1741-1748.
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