A rat decellularized small bowel scaffold that preserves villus-crypt architecture for intestinal regeneration

Giorgia Totonelli, Panagiotis Maghsoudlou, Massimo Garriboli, Johannes Riegler, Giuseppe Orlando, Alan J. Burns, Neil J. Sebire, Virpi V. Smith, Jonathan M. Fishman, Marco Ghionzoli, Mark Turmaine, Martin A. Birchall, Anthony Atala, Shay Soker, Mark F. Lythgoe, Alexander Seifalian, Agostino Pierro, Simon Eaton, Paolo De Coppi

Research output: Contribution to journalArticle

111 Citations (Scopus)

Abstract

Management of intestinal failure remains a clinical challenge and total parenteral nutrition, intestinal elongation and/or transplantation are partial solutions. In this study, using a detergent-enzymatic treatment (DET), we optimize in rats a new protocol that creates a natural intestinal scaffold, as a base for developing functional intestinal tissue. After 1 cycle of DET, histological examination and SEM and TEM analyses showed removal of cellular elements with preservation of the native architecture and connective tissue components. Maintenance of biomechanical, adhesion and angiogenic properties were also demonstrated strengthen the idea that matrices obtained using DET may represent a valid support for intestinal regeneration.

Original languageEnglish (US)
Pages (from-to)3401-3410
Number of pages10
JournalBiomaterials
Volume33
Issue number12
DOIs
StatePublished - Apr 2012
Externally publishedYes

Fingerprint

Detergents
Scaffolds
Rats
Regeneration
Tissue
Total Parenteral Nutrition
Nutrition
Connective Tissue
Elongation
Therapeutics
Adhesion
Transplantation
Maintenance
Transmission electron microscopy
Scanning electron microscopy

Keywords

  • Decellularization
  • Gut transplantation
  • Intestinal failure
  • Natural acellular matrix
  • Regenerative medicine
  • Tissue engineering

ASJC Scopus subject areas

  • Biomaterials
  • Bioengineering
  • Ceramics and Composites
  • Mechanics of Materials
  • Biophysics

Cite this

Totonelli, G., Maghsoudlou, P., Garriboli, M., Riegler, J., Orlando, G., Burns, A. J., ... De Coppi, P. (2012). A rat decellularized small bowel scaffold that preserves villus-crypt architecture for intestinal regeneration. Biomaterials, 33(12), 3401-3410. https://doi.org/10.1016/j.biomaterials.2012.01.012

A rat decellularized small bowel scaffold that preserves villus-crypt architecture for intestinal regeneration. / Totonelli, Giorgia; Maghsoudlou, Panagiotis; Garriboli, Massimo; Riegler, Johannes; Orlando, Giuseppe; Burns, Alan J.; Sebire, Neil J.; Smith, Virpi V.; Fishman, Jonathan M.; Ghionzoli, Marco; Turmaine, Mark; Birchall, Martin A.; Atala, Anthony; Soker, Shay; Lythgoe, Mark F.; Seifalian, Alexander; Pierro, Agostino; Eaton, Simon; De Coppi, Paolo.

In: Biomaterials, Vol. 33, No. 12, 04.2012, p. 3401-3410.

Research output: Contribution to journalArticle

Totonelli, G, Maghsoudlou, P, Garriboli, M, Riegler, J, Orlando, G, Burns, AJ, Sebire, NJ, Smith, VV, Fishman, JM, Ghionzoli, M, Turmaine, M, Birchall, MA, Atala, A, Soker, S, Lythgoe, MF, Seifalian, A, Pierro, A, Eaton, S & De Coppi, P 2012, 'A rat decellularized small bowel scaffold that preserves villus-crypt architecture for intestinal regeneration', Biomaterials, vol. 33, no. 12, pp. 3401-3410. https://doi.org/10.1016/j.biomaterials.2012.01.012
Totonelli, Giorgia ; Maghsoudlou, Panagiotis ; Garriboli, Massimo ; Riegler, Johannes ; Orlando, Giuseppe ; Burns, Alan J. ; Sebire, Neil J. ; Smith, Virpi V. ; Fishman, Jonathan M. ; Ghionzoli, Marco ; Turmaine, Mark ; Birchall, Martin A. ; Atala, Anthony ; Soker, Shay ; Lythgoe, Mark F. ; Seifalian, Alexander ; Pierro, Agostino ; Eaton, Simon ; De Coppi, Paolo. / A rat decellularized small bowel scaffold that preserves villus-crypt architecture for intestinal regeneration. In: Biomaterials. 2012 ; Vol. 33, No. 12. pp. 3401-3410.
@article{cb978c721a6e49ab890daae3b47e4af8,
title = "A rat decellularized small bowel scaffold that preserves villus-crypt architecture for intestinal regeneration",
abstract = "Management of intestinal failure remains a clinical challenge and total parenteral nutrition, intestinal elongation and/or transplantation are partial solutions. In this study, using a detergent-enzymatic treatment (DET), we optimize in rats a new protocol that creates a natural intestinal scaffold, as a base for developing functional intestinal tissue. After 1 cycle of DET, histological examination and SEM and TEM analyses showed removal of cellular elements with preservation of the native architecture and connective tissue components. Maintenance of biomechanical, adhesion and angiogenic properties were also demonstrated strengthen the idea that matrices obtained using DET may represent a valid support for intestinal regeneration.",
keywords = "Decellularization, Gut transplantation, Intestinal failure, Natural acellular matrix, Regenerative medicine, Tissue engineering",
author = "Giorgia Totonelli and Panagiotis Maghsoudlou and Massimo Garriboli and Johannes Riegler and Giuseppe Orlando and Burns, {Alan J.} and Sebire, {Neil J.} and Smith, {Virpi V.} and Fishman, {Jonathan M.} and Marco Ghionzoli and Mark Turmaine and Birchall, {Martin A.} and Anthony Atala and Shay Soker and Lythgoe, {Mark F.} and Alexander Seifalian and Agostino Pierro and Simon Eaton and {De Coppi}, Paolo",
year = "2012",
month = "4",
doi = "10.1016/j.biomaterials.2012.01.012",
language = "English (US)",
volume = "33",
pages = "3401--3410",
journal = "Biomaterials",
issn = "0142-9612",
publisher = "Elsevier BV",
number = "12",

}

TY - JOUR

T1 - A rat decellularized small bowel scaffold that preserves villus-crypt architecture for intestinal regeneration

AU - Totonelli, Giorgia

AU - Maghsoudlou, Panagiotis

AU - Garriboli, Massimo

AU - Riegler, Johannes

AU - Orlando, Giuseppe

AU - Burns, Alan J.

AU - Sebire, Neil J.

AU - Smith, Virpi V.

AU - Fishman, Jonathan M.

AU - Ghionzoli, Marco

AU - Turmaine, Mark

AU - Birchall, Martin A.

AU - Atala, Anthony

AU - Soker, Shay

AU - Lythgoe, Mark F.

AU - Seifalian, Alexander

AU - Pierro, Agostino

AU - Eaton, Simon

AU - De Coppi, Paolo

PY - 2012/4

Y1 - 2012/4

N2 - Management of intestinal failure remains a clinical challenge and total parenteral nutrition, intestinal elongation and/or transplantation are partial solutions. In this study, using a detergent-enzymatic treatment (DET), we optimize in rats a new protocol that creates a natural intestinal scaffold, as a base for developing functional intestinal tissue. After 1 cycle of DET, histological examination and SEM and TEM analyses showed removal of cellular elements with preservation of the native architecture and connective tissue components. Maintenance of biomechanical, adhesion and angiogenic properties were also demonstrated strengthen the idea that matrices obtained using DET may represent a valid support for intestinal regeneration.

AB - Management of intestinal failure remains a clinical challenge and total parenteral nutrition, intestinal elongation and/or transplantation are partial solutions. In this study, using a detergent-enzymatic treatment (DET), we optimize in rats a new protocol that creates a natural intestinal scaffold, as a base for developing functional intestinal tissue. After 1 cycle of DET, histological examination and SEM and TEM analyses showed removal of cellular elements with preservation of the native architecture and connective tissue components. Maintenance of biomechanical, adhesion and angiogenic properties were also demonstrated strengthen the idea that matrices obtained using DET may represent a valid support for intestinal regeneration.

KW - Decellularization

KW - Gut transplantation

KW - Intestinal failure

KW - Natural acellular matrix

KW - Regenerative medicine

KW - Tissue engineering

UR - http://www.scopus.com/inward/record.url?scp=84857043706&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84857043706&partnerID=8YFLogxK

U2 - 10.1016/j.biomaterials.2012.01.012

DO - 10.1016/j.biomaterials.2012.01.012

M3 - Article

VL - 33

SP - 3401

EP - 3410

JO - Biomaterials

JF - Biomaterials

SN - 0142-9612

IS - 12

ER -