A rapid tube ELISA for human IgG in body fluids using Staphylococcus aureus

A. K. Singh, Sudesh P Makker

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

An ELISA was designed for measurement of human IgG in urine and CSF using protein A containing Staphylococcus aureus as sorbent. This assay was performed in test tubes. An OD difference of approximately 1.0 was developed between non-specific binding and 10 μg/ml IgG solution. The method has a sensitivity of 70 ng IgG/ml. The test is simple and rapid and does not require concentration of samples. Once prepared S. aureus reagent is stable for at least one year. The merits of this method in comparison to radial immunodiffusion (RID) are discussed.

Original languageEnglish (US)
Pages (from-to)271-277
Number of pages7
JournalClinica Chimica Acta
Volume129
Issue number3
DOIs
StatePublished - Apr 25 1983
Externally publishedYes

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Body fluids
Body Fluids
Staphylococcus aureus
Immunoglobulin G
Enzyme-Linked Immunosorbent Assay
Immunodiffusion
Staphylococcal Protein A
Sorbents
Assays
Urine

ASJC Scopus subject areas

  • Biochemistry
  • Clinical Biochemistry

Cite this

A rapid tube ELISA for human IgG in body fluids using Staphylococcus aureus. / Singh, A. K.; Makker, Sudesh P.

In: Clinica Chimica Acta, Vol. 129, No. 3, 25.04.1983, p. 271-277.

Research output: Contribution to journalArticle

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