Abstract
One hundred two fecal specimens from psittacine birds submitted to Veterinary Laboratory Services of the California Department of Food and Agriculture at Petaluma were screened for Chlamydia psittaci by a direct immunofluorescence assay using a fluorescein-labeled monoclonal antibody conjugate specific for Chlamydia sp. Results were compared with those obtained by isolation of chlamydia in cultures of McCoy mouse cells. The relative specificity of the direct fluorescent antibody test on fecal smears was 98.9% and the relative sensitivity was 62.5%. The results of this study suggested that the direct fluorescent antibody test was highly specific, and it proved to be a useful same-day antemortem diagnostic test for birds with symptomatic chlamydial infection. The use of centrifugation in the cell culture assay was found to significantly enhance the level of chlamydial infection in cell culture.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 150-153 |
| Number of pages | 4 |
| Journal | Journal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc |
| Volume | 1 |
| Issue number | 2 |
| State | Published - Apr 1989 |
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ASJC Scopus subject areas
- Microbiology
- veterinary(all)
Cite this
A rapid monoclonal immunofluorescence assay for Chlamydia psittaci in fecal smears from psittacine birds. / Woods, Leslie; Dotson, J. F.; Castro, A. E.
In: Journal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc, Vol. 1, No. 2, 04.1989, p. 150-153.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - A rapid monoclonal immunofluorescence assay for Chlamydia psittaci in fecal smears from psittacine birds.
AU - Woods, Leslie
AU - Dotson, J. F.
AU - Castro, A. E.
PY - 1989/4
Y1 - 1989/4
N2 - One hundred two fecal specimens from psittacine birds submitted to Veterinary Laboratory Services of the California Department of Food and Agriculture at Petaluma were screened for Chlamydia psittaci by a direct immunofluorescence assay using a fluorescein-labeled monoclonal antibody conjugate specific for Chlamydia sp. Results were compared with those obtained by isolation of chlamydia in cultures of McCoy mouse cells. The relative specificity of the direct fluorescent antibody test on fecal smears was 98.9% and the relative sensitivity was 62.5%. The results of this study suggested that the direct fluorescent antibody test was highly specific, and it proved to be a useful same-day antemortem diagnostic test for birds with symptomatic chlamydial infection. The use of centrifugation in the cell culture assay was found to significantly enhance the level of chlamydial infection in cell culture.
AB - One hundred two fecal specimens from psittacine birds submitted to Veterinary Laboratory Services of the California Department of Food and Agriculture at Petaluma were screened for Chlamydia psittaci by a direct immunofluorescence assay using a fluorescein-labeled monoclonal antibody conjugate specific for Chlamydia sp. Results were compared with those obtained by isolation of chlamydia in cultures of McCoy mouse cells. The relative specificity of the direct fluorescent antibody test on fecal smears was 98.9% and the relative sensitivity was 62.5%. The results of this study suggested that the direct fluorescent antibody test was highly specific, and it proved to be a useful same-day antemortem diagnostic test for birds with symptomatic chlamydial infection. The use of centrifugation in the cell culture assay was found to significantly enhance the level of chlamydial infection in cell culture.
UR - http://www.scopus.com/inward/record.url?scp=0024648482&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0024648482&partnerID=8YFLogxK
M3 - Article
VL - 1
SP - 150
EP - 153
JO - Journal of Veterinary Diagnostic Investigation
JF - Journal of Veterinary Diagnostic Investigation
SN - 1040-6387
IS - 2
ER -