A rapid monoclonal immunofluorescence assay for Chlamydia psittaci in fecal smears from psittacine birds.

Leslie Woods, J. F. Dotson, A. E. Castro

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

One hundred two fecal specimens from psittacine birds submitted to Veterinary Laboratory Services of the California Department of Food and Agriculture at Petaluma were screened for Chlamydia psittaci by a direct immunofluorescence assay using a fluorescein-labeled monoclonal antibody conjugate specific for Chlamydia sp. Results were compared with those obtained by isolation of chlamydia in cultures of McCoy mouse cells. The relative specificity of the direct fluorescent antibody test on fecal smears was 98.9% and the relative sensitivity was 62.5%. The results of this study suggested that the direct fluorescent antibody test was highly specific, and it proved to be a useful same-day antemortem diagnostic test for birds with symptomatic chlamydial infection. The use of centrifugation in the cell culture assay was found to significantly enhance the level of chlamydial infection in cell culture.

Original languageEnglish (US)
Pages (from-to)150-153
Number of pages4
JournalJournal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc
Volume1
Issue number2
StatePublished - Apr 1989

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Psittaciformes
Chlamydophila psittaci
Chlamydia
fluorescent antibody technique
Birds
Fluorescent Antibody Technique
cell culture
Cell Culture Techniques
Direct Fluorescent Antibody Technique
antibodies
Antibodies
birds
assays
fluorescein
Infection
Agriculture
Fluorescein
Centrifugation
Routine Diagnostic Tests
infection

ASJC Scopus subject areas

  • Microbiology
  • veterinary(all)

Cite this

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abstract = "One hundred two fecal specimens from psittacine birds submitted to Veterinary Laboratory Services of the California Department of Food and Agriculture at Petaluma were screened for Chlamydia psittaci by a direct immunofluorescence assay using a fluorescein-labeled monoclonal antibody conjugate specific for Chlamydia sp. Results were compared with those obtained by isolation of chlamydia in cultures of McCoy mouse cells. The relative specificity of the direct fluorescent antibody test on fecal smears was 98.9{\%} and the relative sensitivity was 62.5{\%}. The results of this study suggested that the direct fluorescent antibody test was highly specific, and it proved to be a useful same-day antemortem diagnostic test for birds with symptomatic chlamydial infection. The use of centrifugation in the cell culture assay was found to significantly enhance the level of chlamydial infection in cell culture.",
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