A rapid assay for insect juvenile hormone esterase activity

Bruce D. Hammock; Thomas C. Sparks

doi:10.1016/0003-2697(77)90196-8

A rapid assay for insect juvenile hormone esterase activity

Bruce D. Hammock, Thomas C. Sparks

Entomology

Research output: Contribution to journal › Article › peer-review

285 Scopus citations

Abstract

A rapid new assay is proposed for analyzing insect juvenile hormone esterase activity. The assay is based on . 99% of radiolabeled juvenile hormone being extracted into an isooctane phase while > 99% of the juvenile hormone acid remains in the basic aqueous-methanol phase. The assay is more rapid and less expensive than conventional chromatographic assays while yielding almost identical values of similar precision. This is the first rapid assay proposed for isomerically pure, commercially available juvenile hormone I.

Original language	English (US)
Pages (from-to)	573-579
Number of pages	7
Journal	Analytical Biochemistry
Volume	82
Issue number	2
DOIs	https://doi.org/10.1016/0003-2697(77)90196-8
State	Published - 1977

ASJC Scopus subject areas

Biochemistry
Biophysics
Molecular Biology

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10.1016/0003-2697(77)90196-8

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title = "A rapid assay for insect juvenile hormone esterase activity",

abstract = "A rapid new assay is proposed for analyzing insect juvenile hormone esterase activity. The assay is based on . 99% of radiolabeled juvenile hormone being extracted into an isooctane phase while > 99% of the juvenile hormone acid remains in the basic aqueous-methanol phase. The assay is more rapid and less expensive than conventional chromatographic assays while yielding almost identical values of similar precision. This is the first rapid assay proposed for isomerically pure, commercially available juvenile hormone I.",

author = "Hammock, {Bruce D.} and Sparks, {Thomas C.}",

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AB - A rapid new assay is proposed for analyzing insect juvenile hormone esterase activity. The assay is based on . 99% of radiolabeled juvenile hormone being extracted into an isooctane phase while > 99% of the juvenile hormone acid remains in the basic aqueous-methanol phase. The assay is more rapid and less expensive than conventional chromatographic assays while yielding almost identical values of similar precision. This is the first rapid assay proposed for isomerically pure, commercially available juvenile hormone I.

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A rapid assay for insect juvenile hormone esterase activity

Abstract

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