A rapid assay for insect juvenile hormone esterase activity

Bruce D. Hammock, Thomas C. Sparks

Research output: Contribution to journalArticle

273 Citations (Scopus)

Abstract

A rapid new assay is proposed for analyzing insect juvenile hormone esterase activity. The assay is based on . 99% of radiolabeled juvenile hormone being extracted into an isooctane phase while > 99% of the juvenile hormone acid remains in the basic aqueous-methanol phase. The assay is more rapid and less expensive than conventional chromatographic assays while yielding almost identical values of similar precision. This is the first rapid assay proposed for isomerically pure, commercially available juvenile hormone I.

Original languageEnglish (US)
Pages (from-to)573-579
Number of pages7
JournalAnalytical Biochemistry
Volume82
Issue number2
DOIs
StatePublished - 1977

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Insect Hormones
Juvenile Hormones
Assays
Methanol
Acids
juvenile hormone esterase

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

A rapid assay for insect juvenile hormone esterase activity. / Hammock, Bruce D.; Sparks, Thomas C.

In: Analytical Biochemistry, Vol. 82, No. 2, 1977, p. 573-579.

Research output: Contribution to journalArticle

Hammock, Bruce D. ; Sparks, Thomas C. / A rapid assay for insect juvenile hormone esterase activity. In: Analytical Biochemistry. 1977 ; Vol. 82, No. 2. pp. 573-579.
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