Abstract
A rapid new assay is proposed for analyzing insect juvenile hormone esterase activity. The assay is based on . 99% of radiolabeled juvenile hormone being extracted into an isooctane phase while > 99% of the juvenile hormone acid remains in the basic aqueous-methanol phase. The assay is more rapid and less expensive than conventional chromatographic assays while yielding almost identical values of similar precision. This is the first rapid assay proposed for isomerically pure, commercially available juvenile hormone I.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 573-579 |
| Number of pages | 7 |
| Journal | Analytical Biochemistry |
| Volume | 82 |
| Issue number | 2 |
| DOIs | |
| State | Published - 1977 |
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ASJC Scopus subject areas
- Biochemistry
- Biophysics
- Molecular Biology
Cite this
A rapid assay for insect juvenile hormone esterase activity. / Hammock, Bruce D.; Sparks, Thomas C.
In: Analytical Biochemistry, Vol. 82, No. 2, 1977, p. 573-579.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - A rapid assay for insect juvenile hormone esterase activity
AU - Hammock, Bruce D.
AU - Sparks, Thomas C.
PY - 1977
Y1 - 1977
N2 - A rapid new assay is proposed for analyzing insect juvenile hormone esterase activity. The assay is based on . 99% of radiolabeled juvenile hormone being extracted into an isooctane phase while > 99% of the juvenile hormone acid remains in the basic aqueous-methanol phase. The assay is more rapid and less expensive than conventional chromatographic assays while yielding almost identical values of similar precision. This is the first rapid assay proposed for isomerically pure, commercially available juvenile hormone I.
AB - A rapid new assay is proposed for analyzing insect juvenile hormone esterase activity. The assay is based on . 99% of radiolabeled juvenile hormone being extracted into an isooctane phase while > 99% of the juvenile hormone acid remains in the basic aqueous-methanol phase. The assay is more rapid and less expensive than conventional chromatographic assays while yielding almost identical values of similar precision. This is the first rapid assay proposed for isomerically pure, commercially available juvenile hormone I.
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UR - http://www.scopus.com/inward/citedby.url?scp=0017660868&partnerID=8YFLogxK
U2 - 10.1016/0003-2697(77)90196-8
DO - 10.1016/0003-2697(77)90196-8
M3 - Article
C2 - 907152
AN - SCOPUS:0017660868
VL - 82
SP - 573
EP - 579
JO - Analytical Biochemistry
JF - Analytical Biochemistry
SN - 0003-2697
IS - 2
ER -