Abstract
OBJECTIVE:: To compare the effect of 2 prebiotic/probiotic products on weight gain, stool microbiota, and stool short-chain fatty acid (SCFA) content of premature infants. PATIENTS AND METHODS:: This randomized, blinded, placebo-controlled trial included 90 premature infants treated with either a dietary supplement containing 2 lactobacillus species plus fructooligosaccharides (CUL, Culturelle, ConAgra, Omaha, NE), a supplement containing several species of lactobacilli and bifidobacteria plus fructooligosaccharides (PBP, ProBioPlus DDS, UAS Laboratories, Eden Prairie, MN), or placebo (a dilute preparation of Pregestamil formula) twice daily for 28 days or until discharge if earlier. The primary outcome was weight gain. Secondary outcomes were stool bacterial analysis by culture and 16S rDNA quantitative polymerase chain reaction and stool SCFA content measured by high performance liquid chromatography. RESULTS:: Both prebiotic/probiotic combinations contained more bacterial species than noted on the label. No significant effect on infant growth of either prebiotic/probiotic supplement was observed. By cultures, 64% of infants receiving PBP became colonized with bifidobacteria, compared with 18% of infants receiving CUL and 27% of infants receiving placebo (chi-square, P = 0.064). No differences were noted between groups in colonization rates for lactobacilli, Gram-negative enteric bacteria, or staphylococci. By 16S rDNA polymerase chain reaction analysis, the bifidobacteria content in the stools of the infants receiving PBP was higher than in the infants receiving CUL or placebo (Kruskal-Wallis, P = 0.011). No significant differences in stool SCFA content were detected between groups. No adverse reactions were noted. CONCLUSIONS:: Infants receiving PBP were more likely to become colonized with bifidobacteria. No significant differences in weight gain or stool SCFA content were detected.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 216-225 |
| Number of pages | 10 |
| Journal | Journal of Pediatric Gastroenterology and Nutrition |
| Volume | 48 |
| Issue number | 2 |
| DOIs | |
| State | Published - Feb 2009 |
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Keywords
- Microflora
- Prebiotic
- Premature infant
- Probiotic
- Synbiotic
ASJC Scopus subject areas
- Gastroenterology
- Pediatrics, Perinatology, and Child Health
Cite this
A randomized placebo-controlled comparison of 2 prebiotic/probiotic combinations in preterm infants : Impact on weight gain, intestinal microbiota, and fecal short-chain fatty acids. / Underwood, Mark; Salzman, Nita H.; Bennett, Stephen H.; Barman, Melissa; Mills, David A.; Marcobal, Angela; Tancredi, Daniel J; Bevins, Charles L; Sherman, Michael P.
In: Journal of Pediatric Gastroenterology and Nutrition, Vol. 48, No. 2, 02.2009, p. 216-225.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - A randomized placebo-controlled comparison of 2 prebiotic/probiotic combinations in preterm infants
T2 - Impact on weight gain, intestinal microbiota, and fecal short-chain fatty acids
AU - Underwood, Mark
AU - Salzman, Nita H.
AU - Bennett, Stephen H.
AU - Barman, Melissa
AU - Mills, David A.
AU - Marcobal, Angela
AU - Tancredi, Daniel J
AU - Bevins, Charles L
AU - Sherman, Michael P.
PY - 2009/2
Y1 - 2009/2
N2 - OBJECTIVE:: To compare the effect of 2 prebiotic/probiotic products on weight gain, stool microbiota, and stool short-chain fatty acid (SCFA) content of premature infants. PATIENTS AND METHODS:: This randomized, blinded, placebo-controlled trial included 90 premature infants treated with either a dietary supplement containing 2 lactobacillus species plus fructooligosaccharides (CUL, Culturelle, ConAgra, Omaha, NE), a supplement containing several species of lactobacilli and bifidobacteria plus fructooligosaccharides (PBP, ProBioPlus DDS, UAS Laboratories, Eden Prairie, MN), or placebo (a dilute preparation of Pregestamil formula) twice daily for 28 days or until discharge if earlier. The primary outcome was weight gain. Secondary outcomes were stool bacterial analysis by culture and 16S rDNA quantitative polymerase chain reaction and stool SCFA content measured by high performance liquid chromatography. RESULTS:: Both prebiotic/probiotic combinations contained more bacterial species than noted on the label. No significant effect on infant growth of either prebiotic/probiotic supplement was observed. By cultures, 64% of infants receiving PBP became colonized with bifidobacteria, compared with 18% of infants receiving CUL and 27% of infants receiving placebo (chi-square, P = 0.064). No differences were noted between groups in colonization rates for lactobacilli, Gram-negative enteric bacteria, or staphylococci. By 16S rDNA polymerase chain reaction analysis, the bifidobacteria content in the stools of the infants receiving PBP was higher than in the infants receiving CUL or placebo (Kruskal-Wallis, P = 0.011). No significant differences in stool SCFA content were detected between groups. No adverse reactions were noted. CONCLUSIONS:: Infants receiving PBP were more likely to become colonized with bifidobacteria. No significant differences in weight gain or stool SCFA content were detected.
AB - OBJECTIVE:: To compare the effect of 2 prebiotic/probiotic products on weight gain, stool microbiota, and stool short-chain fatty acid (SCFA) content of premature infants. PATIENTS AND METHODS:: This randomized, blinded, placebo-controlled trial included 90 premature infants treated with either a dietary supplement containing 2 lactobacillus species plus fructooligosaccharides (CUL, Culturelle, ConAgra, Omaha, NE), a supplement containing several species of lactobacilli and bifidobacteria plus fructooligosaccharides (PBP, ProBioPlus DDS, UAS Laboratories, Eden Prairie, MN), or placebo (a dilute preparation of Pregestamil formula) twice daily for 28 days or until discharge if earlier. The primary outcome was weight gain. Secondary outcomes were stool bacterial analysis by culture and 16S rDNA quantitative polymerase chain reaction and stool SCFA content measured by high performance liquid chromatography. RESULTS:: Both prebiotic/probiotic combinations contained more bacterial species than noted on the label. No significant effect on infant growth of either prebiotic/probiotic supplement was observed. By cultures, 64% of infants receiving PBP became colonized with bifidobacteria, compared with 18% of infants receiving CUL and 27% of infants receiving placebo (chi-square, P = 0.064). No differences were noted between groups in colonization rates for lactobacilli, Gram-negative enteric bacteria, or staphylococci. By 16S rDNA polymerase chain reaction analysis, the bifidobacteria content in the stools of the infants receiving PBP was higher than in the infants receiving CUL or placebo (Kruskal-Wallis, P = 0.011). No significant differences in stool SCFA content were detected between groups. No adverse reactions were noted. CONCLUSIONS:: Infants receiving PBP were more likely to become colonized with bifidobacteria. No significant differences in weight gain or stool SCFA content were detected.
KW - Microflora
KW - Prebiotic
KW - Premature infant
KW - Probiotic
KW - Synbiotic
UR - http://www.scopus.com/inward/record.url?scp=66149153878&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=66149153878&partnerID=8YFLogxK
U2 - 10.1097/MPG.0b013e31818de195
DO - 10.1097/MPG.0b013e31818de195
M3 - Article
C2 - 19179885
AN - SCOPUS:66149153878
VL - 48
SP - 216
EP - 225
JO - Journal of Pediatric Gastroenterology and Nutrition
JF - Journal of Pediatric Gastroenterology and Nutrition
SN - 0277-2116
IS - 2
ER -