A putative role for eotaxin and RANTES in primary biliary cirrhosis: Eosinophilic infiltration and damaged bile ducts

Koichi Tsuneyama, Mitsue Yasoshima, Katsushi Hiramatsu, Kenichi Harada, M. Eric Gershwin, Yasuni Nakanuma

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Eosinophilic infiltration of the portal tracts is frequently seen in the early stages of primary biliary cirrhosis. The mechanism of this infiltration is unclear. Nonetheless it is likely that chemokines and/or their cognante receptors are involved. Multiple C-C chemokines including monocyte chemoattractant protein (MCP)-2, MCP-3, MCP-4, RANTES and eotaxin are known to target eosinophils. Eotaxin, in particular, is unique among C-C chemokines because eotaxin activity is limited to eosinophils. In fact eotaxin has been proposed to be both an eosinophil activator and a chemoattractor. In this study, we examined the expression of eotaxin and RANTES in various liver diseases to clarify the mechanism of eosinophilic infiltration in primary biliary cirrhosis. Our data suggest that RANTES is commonly expressed on biliary epithelial cells even in normal livers. In contrast eotaxin was not uniformly expressed on biliary epithelial cells and hepatocytes. Interestingly, however, there were a significant number of eotaxin positive mononuclear cells infiltrated in the portal tract and, especially, near damaged bile ducts in patients with primary biliary cirrhosis. Moreover, using double immunostaining, most eotaxin positive cells near damaged ducts co-expressed CD68, a marker of macrophages. Our data suggest that generic biliary expression of RANTES may play a role in focusing and or homing of precursor macrophages and eosinophils near biliary epithelial cells. In addition, in response to inflammation and/or infection of biliary epithelial cells, these inflammatory cells may become activated and thence express eotaxin; this leads to recruitment of eosinophils around the damaged bile ducts. Clarification of the initial events responsible for eotaxin expression around damaged bile ducts may be a clue to the pathogenesis of primary biliary cirrhosis.

Original languageEnglish (US)
Pages (from-to)68-77
Number of pages10
JournalHepatology Research
Volume16
Issue number1
DOIs
StatePublished - Oct 1999

Fingerprint

Chemokine CCL5
Biliary Liver Cirrhosis
Bile Ducts
Eosinophils
Epithelial Cells
CC Chemokines
Chemokine CCL8
Chemokine CCL7
Monocyte Chemoattractant Proteins
Macrophages
Chemokines
Liver Diseases
Hepatocytes
Inflammation
Liver
Infection

Keywords

  • Chemokine
  • Eotaxin
  • Primary biliary cirrhosis
  • RANTES

ASJC Scopus subject areas

  • Gastroenterology

Cite this

A putative role for eotaxin and RANTES in primary biliary cirrhosis : Eosinophilic infiltration and damaged bile ducts. / Tsuneyama, Koichi; Yasoshima, Mitsue; Hiramatsu, Katsushi; Harada, Kenichi; Gershwin, M. Eric; Nakanuma, Yasuni.

In: Hepatology Research, Vol. 16, No. 1, 10.1999, p. 68-77.

Research output: Contribution to journalArticle

Tsuneyama, Koichi ; Yasoshima, Mitsue ; Hiramatsu, Katsushi ; Harada, Kenichi ; Gershwin, M. Eric ; Nakanuma, Yasuni. / A putative role for eotaxin and RANTES in primary biliary cirrhosis : Eosinophilic infiltration and damaged bile ducts. In: Hepatology Research. 1999 ; Vol. 16, No. 1. pp. 68-77.
@article{2cfd19aa3873463585d6ff92c43297cb,
title = "A putative role for eotaxin and RANTES in primary biliary cirrhosis: Eosinophilic infiltration and damaged bile ducts",
abstract = "Eosinophilic infiltration of the portal tracts is frequently seen in the early stages of primary biliary cirrhosis. The mechanism of this infiltration is unclear. Nonetheless it is likely that chemokines and/or their cognante receptors are involved. Multiple C-C chemokines including monocyte chemoattractant protein (MCP)-2, MCP-3, MCP-4, RANTES and eotaxin are known to target eosinophils. Eotaxin, in particular, is unique among C-C chemokines because eotaxin activity is limited to eosinophils. In fact eotaxin has been proposed to be both an eosinophil activator and a chemoattractor. In this study, we examined the expression of eotaxin and RANTES in various liver diseases to clarify the mechanism of eosinophilic infiltration in primary biliary cirrhosis. Our data suggest that RANTES is commonly expressed on biliary epithelial cells even in normal livers. In contrast eotaxin was not uniformly expressed on biliary epithelial cells and hepatocytes. Interestingly, however, there were a significant number of eotaxin positive mononuclear cells infiltrated in the portal tract and, especially, near damaged bile ducts in patients with primary biliary cirrhosis. Moreover, using double immunostaining, most eotaxin positive cells near damaged ducts co-expressed CD68, a marker of macrophages. Our data suggest that generic biliary expression of RANTES may play a role in focusing and or homing of precursor macrophages and eosinophils near biliary epithelial cells. In addition, in response to inflammation and/or infection of biliary epithelial cells, these inflammatory cells may become activated and thence express eotaxin; this leads to recruitment of eosinophils around the damaged bile ducts. Clarification of the initial events responsible for eotaxin expression around damaged bile ducts may be a clue to the pathogenesis of primary biliary cirrhosis.",
keywords = "Chemokine, Eotaxin, Primary biliary cirrhosis, RANTES",
author = "Koichi Tsuneyama and Mitsue Yasoshima and Katsushi Hiramatsu and Kenichi Harada and Gershwin, {M. Eric} and Yasuni Nakanuma",
year = "1999",
month = "10",
doi = "10.1016/S1386-6346(99)00042-X",
language = "English (US)",
volume = "16",
pages = "68--77",
journal = "International Hepatology Communications",
issn = "0928-4346",
publisher = "Wiley-Blackwell",
number = "1",

}

TY - JOUR

T1 - A putative role for eotaxin and RANTES in primary biliary cirrhosis

T2 - Eosinophilic infiltration and damaged bile ducts

AU - Tsuneyama, Koichi

AU - Yasoshima, Mitsue

AU - Hiramatsu, Katsushi

AU - Harada, Kenichi

AU - Gershwin, M. Eric

AU - Nakanuma, Yasuni

PY - 1999/10

Y1 - 1999/10

N2 - Eosinophilic infiltration of the portal tracts is frequently seen in the early stages of primary biliary cirrhosis. The mechanism of this infiltration is unclear. Nonetheless it is likely that chemokines and/or their cognante receptors are involved. Multiple C-C chemokines including monocyte chemoattractant protein (MCP)-2, MCP-3, MCP-4, RANTES and eotaxin are known to target eosinophils. Eotaxin, in particular, is unique among C-C chemokines because eotaxin activity is limited to eosinophils. In fact eotaxin has been proposed to be both an eosinophil activator and a chemoattractor. In this study, we examined the expression of eotaxin and RANTES in various liver diseases to clarify the mechanism of eosinophilic infiltration in primary biliary cirrhosis. Our data suggest that RANTES is commonly expressed on biliary epithelial cells even in normal livers. In contrast eotaxin was not uniformly expressed on biliary epithelial cells and hepatocytes. Interestingly, however, there were a significant number of eotaxin positive mononuclear cells infiltrated in the portal tract and, especially, near damaged bile ducts in patients with primary biliary cirrhosis. Moreover, using double immunostaining, most eotaxin positive cells near damaged ducts co-expressed CD68, a marker of macrophages. Our data suggest that generic biliary expression of RANTES may play a role in focusing and or homing of precursor macrophages and eosinophils near biliary epithelial cells. In addition, in response to inflammation and/or infection of biliary epithelial cells, these inflammatory cells may become activated and thence express eotaxin; this leads to recruitment of eosinophils around the damaged bile ducts. Clarification of the initial events responsible for eotaxin expression around damaged bile ducts may be a clue to the pathogenesis of primary biliary cirrhosis.

AB - Eosinophilic infiltration of the portal tracts is frequently seen in the early stages of primary biliary cirrhosis. The mechanism of this infiltration is unclear. Nonetheless it is likely that chemokines and/or their cognante receptors are involved. Multiple C-C chemokines including monocyte chemoattractant protein (MCP)-2, MCP-3, MCP-4, RANTES and eotaxin are known to target eosinophils. Eotaxin, in particular, is unique among C-C chemokines because eotaxin activity is limited to eosinophils. In fact eotaxin has been proposed to be both an eosinophil activator and a chemoattractor. In this study, we examined the expression of eotaxin and RANTES in various liver diseases to clarify the mechanism of eosinophilic infiltration in primary biliary cirrhosis. Our data suggest that RANTES is commonly expressed on biliary epithelial cells even in normal livers. In contrast eotaxin was not uniformly expressed on biliary epithelial cells and hepatocytes. Interestingly, however, there were a significant number of eotaxin positive mononuclear cells infiltrated in the portal tract and, especially, near damaged bile ducts in patients with primary biliary cirrhosis. Moreover, using double immunostaining, most eotaxin positive cells near damaged ducts co-expressed CD68, a marker of macrophages. Our data suggest that generic biliary expression of RANTES may play a role in focusing and or homing of precursor macrophages and eosinophils near biliary epithelial cells. In addition, in response to inflammation and/or infection of biliary epithelial cells, these inflammatory cells may become activated and thence express eotaxin; this leads to recruitment of eosinophils around the damaged bile ducts. Clarification of the initial events responsible for eotaxin expression around damaged bile ducts may be a clue to the pathogenesis of primary biliary cirrhosis.

KW - Chemokine

KW - Eotaxin

KW - Primary biliary cirrhosis

KW - RANTES

UR - http://www.scopus.com/inward/record.url?scp=0032705161&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0032705161&partnerID=8YFLogxK

U2 - 10.1016/S1386-6346(99)00042-X

DO - 10.1016/S1386-6346(99)00042-X

M3 - Article

AN - SCOPUS:0032705161

VL - 16

SP - 68

EP - 77

JO - International Hepatology Communications

JF - International Hepatology Communications

SN - 0928-4346

IS - 1

ER -