A putative ATP binding protein influences the fidelity of branchpoint recognition in yeast splicing

Sean Burgess, Joseph R. Couto, Christine Guthrie

Research output: Contribution to journalArticle

135 Citations (Scopus)

Abstract

We previously described a dominant suppressor of the splicing defect conferred by an A→;C intron branchpoint mutation in S. cerevisiae. Suppression occurs by increasing the frequency with which the mutant branchpoint is utilized. We have now cloned the genomic region encoding the prp16-1 suppressor function and have demonstrated that PRP16 is essential for viability. A 1071 amino acid open reading frame contains sequence motifs characteristic of an NTP binding fold and further similarities to a superfamily of proteins that includes members with demonstrated RNA-dependent ATPase activity. A single nucleotide change necessary to confer the prp16-1 suppressor phenotype results in a Tyr→Asp substitution near the "A site" consensus for NTP binding proteins. We propose that PRP16 is an excellent candidate for mediating one of the many ATP-requiring steps of spliceosome assembly and that accuracy of branchpoint recognition may be coupled to ATP binding and/or hydrolysis.

Original languageEnglish (US)
Pages (from-to)705-717
Number of pages13
JournalCell
Volume60
Issue number5
DOIs
StatePublished - Mar 9 1990

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Yeast
Carrier Proteins
Adenosine Triphosphate
Yeasts
Spliceosomes
Introns
Open Reading Frames
Saccharomyces cerevisiae
Hydrolysis
Substitution reactions
Nucleotides
Phenotype
Amino Acids
Defects
Mutation
Proteins
RNA-dependent ATPase

ASJC Scopus subject areas

  • Cell Biology
  • Molecular Biology

Cite this

A putative ATP binding protein influences the fidelity of branchpoint recognition in yeast splicing. / Burgess, Sean; Couto, Joseph R.; Guthrie, Christine.

In: Cell, Vol. 60, No. 5, 09.03.1990, p. 705-717.

Research output: Contribution to journalArticle

Burgess, Sean ; Couto, Joseph R. ; Guthrie, Christine. / A putative ATP binding protein influences the fidelity of branchpoint recognition in yeast splicing. In: Cell. 1990 ; Vol. 60, No. 5. pp. 705-717.
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