A procedure for the immunoanalysis of samples containing one or more members of a group of cross-reacting analytes

Geoffrey Jones, Monika Wortberg, Bruce D. Hammock, David M Rocke

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

The information obtained from a single-antibody immunoassay can be ambiguous when the identity of the analyte is unknown and could be one of a group of compounds all having different affinities for the antibody. If we allow the possibility of mixtures of analytes the difficulty of the situation is aggravated. However, additional information can sometimes be obtained by assaying with a number of different antibodies. We demonstrate a rationale for identifying and quantifying analytes from a group of candidates, and illustrate the application of our method with an example from the class of s-triazine herbicides. Using a four-antibody array, correct identification and accurate quantification were usually achieved for single analytes samples in the low ppb range. Mixtures of analytes were recognized as such but were more difficult to classify correctly, with some confusion arising between members of subgroups, particularly within the methoxy/methylthio substituted triazines. The mathematical perspective is used to suggest directions for improving the experimental performance of multianalyte immunoassay.

Original languageEnglish (US)
Pages (from-to)175-183
Number of pages9
JournalAnalytica Chimica Acta
Volume336
Issue number1-3
DOIs
StatePublished - Dec 30 1996

Fingerprint

antibody
Triazines
Immunoassay
Antibodies
triazine
immunoassay
Antibody Affinity
Herbicides
herbicide
Direction compound

Keywords

  • Cross-reactivity
  • Immunoassay
  • Multianalyte

ASJC Scopus subject areas

  • Biochemistry
  • Analytical Chemistry
  • Spectroscopy
  • Environmental Chemistry

Cite this

A procedure for the immunoanalysis of samples containing one or more members of a group of cross-reacting analytes. / Jones, Geoffrey; Wortberg, Monika; Hammock, Bruce D.; Rocke, David M.

In: Analytica Chimica Acta, Vol. 336, No. 1-3, 30.12.1996, p. 175-183.

Research output: Contribution to journalArticle

Jones, Geoffrey ; Wortberg, Monika ; Hammock, Bruce D. ; Rocke, David M. / A procedure for the immunoanalysis of samples containing one or more members of a group of cross-reacting analytes. In: Analytica Chimica Acta. 1996 ; Vol. 336, No. 1-3. pp. 175-183.
@article{8d6aef2050a948f9be05b42b55dd0a37,
title = "A procedure for the immunoanalysis of samples containing one or more members of a group of cross-reacting analytes",
abstract = "The information obtained from a single-antibody immunoassay can be ambiguous when the identity of the analyte is unknown and could be one of a group of compounds all having different affinities for the antibody. If we allow the possibility of mixtures of analytes the difficulty of the situation is aggravated. However, additional information can sometimes be obtained by assaying with a number of different antibodies. We demonstrate a rationale for identifying and quantifying analytes from a group of candidates, and illustrate the application of our method with an example from the class of s-triazine herbicides. Using a four-antibody array, correct identification and accurate quantification were usually achieved for single analytes samples in the low ppb range. Mixtures of analytes were recognized as such but were more difficult to classify correctly, with some confusion arising between members of subgroups, particularly within the methoxy/methylthio substituted triazines. The mathematical perspective is used to suggest directions for improving the experimental performance of multianalyte immunoassay.",
keywords = "Cross-reactivity, Immunoassay, Multianalyte",
author = "Geoffrey Jones and Monika Wortberg and Hammock, {Bruce D.} and Rocke, {David M}",
year = "1996",
month = "12",
day = "30",
doi = "10.1016/S0003-2670(96)00394-7",
language = "English (US)",
volume = "336",
pages = "175--183",
journal = "Analytica Chimica Acta",
issn = "0003-2670",
publisher = "Elsevier",
number = "1-3",

}

TY - JOUR

T1 - A procedure for the immunoanalysis of samples containing one or more members of a group of cross-reacting analytes

AU - Jones, Geoffrey

AU - Wortberg, Monika

AU - Hammock, Bruce D.

AU - Rocke, David M

PY - 1996/12/30

Y1 - 1996/12/30

N2 - The information obtained from a single-antibody immunoassay can be ambiguous when the identity of the analyte is unknown and could be one of a group of compounds all having different affinities for the antibody. If we allow the possibility of mixtures of analytes the difficulty of the situation is aggravated. However, additional information can sometimes be obtained by assaying with a number of different antibodies. We demonstrate a rationale for identifying and quantifying analytes from a group of candidates, and illustrate the application of our method with an example from the class of s-triazine herbicides. Using a four-antibody array, correct identification and accurate quantification were usually achieved for single analytes samples in the low ppb range. Mixtures of analytes were recognized as such but were more difficult to classify correctly, with some confusion arising between members of subgroups, particularly within the methoxy/methylthio substituted triazines. The mathematical perspective is used to suggest directions for improving the experimental performance of multianalyte immunoassay.

AB - The information obtained from a single-antibody immunoassay can be ambiguous when the identity of the analyte is unknown and could be one of a group of compounds all having different affinities for the antibody. If we allow the possibility of mixtures of analytes the difficulty of the situation is aggravated. However, additional information can sometimes be obtained by assaying with a number of different antibodies. We demonstrate a rationale for identifying and quantifying analytes from a group of candidates, and illustrate the application of our method with an example from the class of s-triazine herbicides. Using a four-antibody array, correct identification and accurate quantification were usually achieved for single analytes samples in the low ppb range. Mixtures of analytes were recognized as such but were more difficult to classify correctly, with some confusion arising between members of subgroups, particularly within the methoxy/methylthio substituted triazines. The mathematical perspective is used to suggest directions for improving the experimental performance of multianalyte immunoassay.

KW - Cross-reactivity

KW - Immunoassay

KW - Multianalyte

UR - http://www.scopus.com/inward/record.url?scp=0030607731&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0030607731&partnerID=8YFLogxK

U2 - 10.1016/S0003-2670(96)00394-7

DO - 10.1016/S0003-2670(96)00394-7

M3 - Article

AN - SCOPUS:0030607731

VL - 336

SP - 175

EP - 183

JO - Analytica Chimica Acta

JF - Analytica Chimica Acta

SN - 0003-2670

IS - 1-3

ER -