A preliminary report on the contact-independent antagonism of Pseudogymnoascus destructans by Rhodococcus rhodochrous strain DAP96253

Christopher T. Cornelison, Michael K Keel, Kyle T. Gabriel, Courtney K. Barlament, Trudy A. Tucker, George E. Pierce, Sidney A. Crow

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

Background: The recently-identified causative agent of White-Nose Syndrome (WNS), Pseudogymnoascus destructans, has been responsible for the mortality of an estimated 5.5 million North American bats since its emergence in 2006. A primary focus of the National Response Plan, established by multiple state, federal and tribal agencies in 2011, was the identification of biological control options for WNS. In an effort to identify potential biological control options for WNS, multiply induced cells of Rhodococcus rhodochrous strain DAP96253 was screened for anti-P. destructans activity.

Results: Conidia and mycelial plugs of P. destructans were exposed to induced R. rhodochrous in a closed air-space at 15°C, 7°C and 4°C and were evaluated for contact-independent inhibition of conidia germination and mycelial extension with positive results. Additionally, in situ application methods for induced R. rhodochrous, such as fixed-cell catalyst and fermentation cell-paste in non-growth conditions, were screened with positive results. R. rhodochrous was assayed for ex vivo activity via exposure to bat tissue explants inoculated with P. destructans conidia. Induced R. rhodochrous completely inhibited growth from conidia at 15°C and had a strong fungistatic effect at 4°C. Induced R. rhodochrous inhibited P. destructans growth from conidia when cultured in a shared air-space with bat tissue explants inoculated with P. destructans conidia.

Conclusion: The identification of inducible biological agents with contact-independent anti- P. destructans activity is a major milestone in the development of viable biological control options for in situ application and provides the first example of contact-independent antagonism of this devastating wildlife pathogen.

Original languageEnglish (US)
Article number246
JournalBMC Microbiology
Volume14
Issue number1
DOIs
StatePublished - Sep 26 2014

Fingerprint

Rhodococcus
Fungal Spores
Nose
Air
Contact Inhibition
Biological Factors
Growth
Germination
Ointments
Fermentation
Mortality

Keywords

  • Biocontrol
  • Conidia
  • Mycelia
  • Pseudogymnoascus destructans
  • Rhodococcus rhodochrous
  • White-Nose Syndrome

ASJC Scopus subject areas

  • Microbiology
  • Microbiology (medical)

Cite this

A preliminary report on the contact-independent antagonism of Pseudogymnoascus destructans by Rhodococcus rhodochrous strain DAP96253. / Cornelison, Christopher T.; Keel, Michael K; Gabriel, Kyle T.; Barlament, Courtney K.; Tucker, Trudy A.; Pierce, George E.; Crow, Sidney A.

In: BMC Microbiology, Vol. 14, No. 1, 246, 26.09.2014.

Research output: Contribution to journalArticle

Cornelison, Christopher T. ; Keel, Michael K ; Gabriel, Kyle T. ; Barlament, Courtney K. ; Tucker, Trudy A. ; Pierce, George E. ; Crow, Sidney A. / A preliminary report on the contact-independent antagonism of Pseudogymnoascus destructans by Rhodococcus rhodochrous strain DAP96253. In: BMC Microbiology. 2014 ; Vol. 14, No. 1.
@article{7b914ea0c0974f2bb8c2b17e8159eba6,
title = "A preliminary report on the contact-independent antagonism of Pseudogymnoascus destructans by Rhodococcus rhodochrous strain DAP96253",
abstract = "Background: The recently-identified causative agent of White-Nose Syndrome (WNS), Pseudogymnoascus destructans, has been responsible for the mortality of an estimated 5.5 million North American bats since its emergence in 2006. A primary focus of the National Response Plan, established by multiple state, federal and tribal agencies in 2011, was the identification of biological control options for WNS. In an effort to identify potential biological control options for WNS, multiply induced cells of Rhodococcus rhodochrous strain DAP96253 was screened for anti-P. destructans activity.Results: Conidia and mycelial plugs of P. destructans were exposed to induced R. rhodochrous in a closed air-space at 15°C, 7°C and 4°C and were evaluated for contact-independent inhibition of conidia germination and mycelial extension with positive results. Additionally, in situ application methods for induced R. rhodochrous, such as fixed-cell catalyst and fermentation cell-paste in non-growth conditions, were screened with positive results. R. rhodochrous was assayed for ex vivo activity via exposure to bat tissue explants inoculated with P. destructans conidia. Induced R. rhodochrous completely inhibited growth from conidia at 15°C and had a strong fungistatic effect at 4°C. Induced R. rhodochrous inhibited P. destructans growth from conidia when cultured in a shared air-space with bat tissue explants inoculated with P. destructans conidia.Conclusion: The identification of inducible biological agents with contact-independent anti- P. destructans activity is a major milestone in the development of viable biological control options for in situ application and provides the first example of contact-independent antagonism of this devastating wildlife pathogen.",
keywords = "Biocontrol, Conidia, Mycelia, Pseudogymnoascus destructans, Rhodococcus rhodochrous, White-Nose Syndrome",
author = "Cornelison, {Christopher T.} and Keel, {Michael K} and Gabriel, {Kyle T.} and Barlament, {Courtney K.} and Tucker, {Trudy A.} and Pierce, {George E.} and Crow, {Sidney A.}",
year = "2014",
month = "9",
day = "26",
doi = "10.1186/s12866-014-0246-y",
language = "English (US)",
volume = "14",
journal = "BMC Microbiology",
issn = "1471-2180",
publisher = "BioMed Central",
number = "1",

}

TY - JOUR

T1 - A preliminary report on the contact-independent antagonism of Pseudogymnoascus destructans by Rhodococcus rhodochrous strain DAP96253

AU - Cornelison, Christopher T.

AU - Keel, Michael K

AU - Gabriel, Kyle T.

AU - Barlament, Courtney K.

AU - Tucker, Trudy A.

AU - Pierce, George E.

AU - Crow, Sidney A.

PY - 2014/9/26

Y1 - 2014/9/26

N2 - Background: The recently-identified causative agent of White-Nose Syndrome (WNS), Pseudogymnoascus destructans, has been responsible for the mortality of an estimated 5.5 million North American bats since its emergence in 2006. A primary focus of the National Response Plan, established by multiple state, federal and tribal agencies in 2011, was the identification of biological control options for WNS. In an effort to identify potential biological control options for WNS, multiply induced cells of Rhodococcus rhodochrous strain DAP96253 was screened for anti-P. destructans activity.Results: Conidia and mycelial plugs of P. destructans were exposed to induced R. rhodochrous in a closed air-space at 15°C, 7°C and 4°C and were evaluated for contact-independent inhibition of conidia germination and mycelial extension with positive results. Additionally, in situ application methods for induced R. rhodochrous, such as fixed-cell catalyst and fermentation cell-paste in non-growth conditions, were screened with positive results. R. rhodochrous was assayed for ex vivo activity via exposure to bat tissue explants inoculated with P. destructans conidia. Induced R. rhodochrous completely inhibited growth from conidia at 15°C and had a strong fungistatic effect at 4°C. Induced R. rhodochrous inhibited P. destructans growth from conidia when cultured in a shared air-space with bat tissue explants inoculated with P. destructans conidia.Conclusion: The identification of inducible biological agents with contact-independent anti- P. destructans activity is a major milestone in the development of viable biological control options for in situ application and provides the first example of contact-independent antagonism of this devastating wildlife pathogen.

AB - Background: The recently-identified causative agent of White-Nose Syndrome (WNS), Pseudogymnoascus destructans, has been responsible for the mortality of an estimated 5.5 million North American bats since its emergence in 2006. A primary focus of the National Response Plan, established by multiple state, federal and tribal agencies in 2011, was the identification of biological control options for WNS. In an effort to identify potential biological control options for WNS, multiply induced cells of Rhodococcus rhodochrous strain DAP96253 was screened for anti-P. destructans activity.Results: Conidia and mycelial plugs of P. destructans were exposed to induced R. rhodochrous in a closed air-space at 15°C, 7°C and 4°C and were evaluated for contact-independent inhibition of conidia germination and mycelial extension with positive results. Additionally, in situ application methods for induced R. rhodochrous, such as fixed-cell catalyst and fermentation cell-paste in non-growth conditions, were screened with positive results. R. rhodochrous was assayed for ex vivo activity via exposure to bat tissue explants inoculated with P. destructans conidia. Induced R. rhodochrous completely inhibited growth from conidia at 15°C and had a strong fungistatic effect at 4°C. Induced R. rhodochrous inhibited P. destructans growth from conidia when cultured in a shared air-space with bat tissue explants inoculated with P. destructans conidia.Conclusion: The identification of inducible biological agents with contact-independent anti- P. destructans activity is a major milestone in the development of viable biological control options for in situ application and provides the first example of contact-independent antagonism of this devastating wildlife pathogen.

KW - Biocontrol

KW - Conidia

KW - Mycelia

KW - Pseudogymnoascus destructans

KW - Rhodococcus rhodochrous

KW - White-Nose Syndrome

UR - http://www.scopus.com/inward/record.url?scp=84907450792&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84907450792&partnerID=8YFLogxK

U2 - 10.1186/s12866-014-0246-y

DO - 10.1186/s12866-014-0246-y

M3 - Article

VL - 14

JO - BMC Microbiology

JF - BMC Microbiology

SN - 1471-2180

IS - 1

M1 - 246

ER -