A positive role in differentiation for the male doublesex protein of Drosophila

Victoria A. Jursnich, Kenneth C. Burtis

Research output: Contribution to journalArticle

43 Citations (Scopus)

Abstract

The doublesex (dsx) locus encodes male-specific and female-specific polypeptides that are essential for the proper differentiation of sexually dimorphic somatic features of Drasophila melanogaster. Ectopic expression of the male-specific dsx polypeptide was obtained by P-element-mediated transformation of flies with a construct bearing a fusion between the hsp70 heat shock promoter and dsx male-specific cDNA sequences. Heat shock-induced expression of the male cDNA in either sex resulted in three novel phenotypes: transformation of bristles on all legs toward a sex comb-like morphology, pigmentation of dorsal spinules and ventral setae in third-instar larvae, and lethality. These results were not predicted by previous models of dsx function, and provide evidence that the role of the male dsx protein includes activation of some aspects of male differentiation as well as repression of female differentiation.

Original languageEnglish (US)
Pages (from-to)235-249
Number of pages15
JournalDevelopmental Biology
Volume155
Issue number1
StatePublished - 1993

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Shock
Complementary DNA
Hot Temperature
Sensilla
Comb and Wattles
Peptides
Pigmentation
Diptera
Larva
Drosophila DSX protein
Leg
Phenotype
Proteins
Ectopic Gene Expression

ASJC Scopus subject areas

  • Developmental Biology

Cite this

A positive role in differentiation for the male doublesex protein of Drosophila. / Jursnich, Victoria A.; Burtis, Kenneth C.

In: Developmental Biology, Vol. 155, No. 1, 1993, p. 235-249.

Research output: Contribution to journalArticle

Jursnich, VA & Burtis, KC 1993, 'A positive role in differentiation for the male doublesex protein of Drosophila', Developmental Biology, vol. 155, no. 1, pp. 235-249.
Jursnich, Victoria A. ; Burtis, Kenneth C. / A positive role in differentiation for the male doublesex protein of Drosophila. In: Developmental Biology. 1993 ; Vol. 155, No. 1. pp. 235-249.
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