A polymerase chain reaction assay for the detection of genomic DNA of a Rickettsiales-like prokaryote associated with withering syndrome in California abalone

Karl B. Andree, Carolyn S. Friedman, James D. Moore, Ronald Hedrick

Research output: Contribution to journalArticle

33 Citations (Scopus)

Abstract

The 16S rDNA from a Rickettsiales-like procaryote (RLP) infecting postesophageal tissues of black abalone Haliotis cracherodii Leach exhibiting signs of withering syndrome (WS) was amplified, cloned, and sequenced. The 16S rDNA sequence for the RLP was similar to that of species found in the genera Ehrlichia, Anaplasma, and Wolbachia. A polymerase chain reaction (PCR) test was developed that specifically amplifies a 160 bp segment of the 16S rDNA from the RLP associated with WS. Positive reactions were obtained for all black abalone samples of digestive gland or postesophagus known to be infected with the RLP by microscopic examinations of stained tissue sections. The PCR worked equally well for infected tissues of black and red abalone H. rufescens Leach. There was no amplification of genomic DNA from four other microbial species isolated from cultures of intestinal flora of abalone or from abalone deemed free of the RLP by microscopic examinations. This PCR test greatly increases the ability to detect the bacterium, because to date no means to grow the organism from marine invertebrates on synthetic media or in cell lines have been developed. This PCR test should allow detection of the RLP before the onset of clinical signs of withering syndrome in cultured or wild abalone stocks. Furthermore, the test may be useful in identifying reservoirs or other related RLPs in other marine invertebrates.

Original languageEnglish (US)
Pages (from-to)213-218
Number of pages6
JournalJournal of Shellfish Research
Volume19
Issue number1
StatePublished - Jun 1 2000

Fingerprint

Rickettsiales
prokaryote
abalone
Haliotis cracherodii
prokaryotic cells
polymerase chain reaction
genomics
assay
DNA
assays
invertebrate
invertebrates
testing
Haliotis rufescens
Anaplasma
Ehrlichia
Wolbachia
intestinal microorganisms
procaryote
detection

Keywords

  • Abalone
  • Ehrlichia
  • Haliotis
  • Polymerase chain reaction
  • Ribosomal DNA
  • Rickettsiales

ASJC Scopus subject areas

  • Aquatic Science

Cite this

A polymerase chain reaction assay for the detection of genomic DNA of a Rickettsiales-like prokaryote associated with withering syndrome in California abalone. / Andree, Karl B.; Friedman, Carolyn S.; Moore, James D.; Hedrick, Ronald.

In: Journal of Shellfish Research, Vol. 19, No. 1, 01.06.2000, p. 213-218.

Research output: Contribution to journalArticle

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